| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 302,171 | A→G | 19.4% | intergenic (‑374/‑44) | yagT ← / → yagU | predicted xanthine dehydrogenase, 2Fe‑2S subunit/conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 302,171 | 0 | A | G | 19.4% | 144.7 / 30.6 | 67 | intergenic (‑374/‑44) | yagT/yagU | predicted xanthine dehydrogenase, 2Fe‑2S subunit/conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (32/22); new base G (8/5); total (40/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
TTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCAC > W3110S.gb/302102‑302239 | ttGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATAc < 1:4178313/71‑1 (MQ=255)ttGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATAc < 1:2269677/71‑1 (MQ=255) aTTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATGCTCCTg < 1:555160/70‑1 (MQ=255) ttAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGaa < 1:1152946/71‑1 (MQ=255) ttAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGa < 1:3330739/70‑1 (MQ=255) tAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATGCTCCTg > 1:1097823/1‑68 (MQ=255) tAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTg > 1:1279211/1‑68 (MQ=255) gAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAgg < 1:3619574/71‑1 (MQ=255) aaaCTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGgt > 1:1995241/1‑71 (MQ=255) aaCTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATGCTCCTGAAGAGgtg > 1:2047627/1‑71 (MQ=255) aaCTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTg > 1:2953657/1‑63 (MQ=255) aaCTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGgtg < 1:2840865/71‑1 (MQ=255) aCTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTg < 1:388745/62‑1 (MQ=255) tCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGgtg > 1:2215768/1‑66 (MQ=255) tCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGtata < 1:2956546/70‑1 (MQ=255) tCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGtat < 1:2101959/69‑1 (MQ=255) cTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGtatat > 1:3566395/1‑69 (MQ=255) ttGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACa > 1:2859086/1‑71 (MQ=255) ttGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACa > 1:713539/1‑71 (MQ=255) tGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACa > 1:4085053/1‑70 (MQ=255) tGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACa > 1:3203857/1‑70 (MQ=255) gTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATaa < 1:1714573/67‑1 (MQ=255) gTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACAt < 1:2951640/70‑1 (MQ=255) ataCAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTc > 1:2515091/1‑48 (MQ=255) ataCAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATg > 1:3374592/1‑71 (MQ=255) ataCAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATg > 1:128512/1‑71 (MQ=255) taCAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATgg < 1:1560661/71‑1 (MQ=255) aCAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAaga < 1:1764691/53‑1 (MQ=255) aCAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAaga < 1:1350164/53‑1 (MQ=255) cAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTa > 1:1686699/1‑71 (MQ=255) aaaaCAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTAc < 1:3973567/71‑1 (MQ=255) aaaCAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAaga > 1:2850606/1‑50 (MQ=255) aCAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAaga > 1:3971154/1‑48 (MQ=255) cAATACAGTTCTTTACATTTGCCTTGTTTTATGAATGCTCCTg < 1:64235/43‑1 (MQ=255) cAATACAGTTCTTTACATTTGCCTTGTTTTATGAATGCTCCTGAAGAGGTGTATAACATAATGGTACAAg > 1:1983735/1‑70 (MQ=255) aTACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAg > 1:2528910/1‑71 (MQ=255) aTACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAg > 1:2512976/1‑71 (MQ=255) aGTTCTTTACATTTGCCTTGTTTTGTGAATACTCCTGAAGAGGTGTATAACa < 1:534334/52‑1 (MQ=255) gTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAggg > 1:4086441/1‑68 (MQ=255) gTTCTTTACATTTCCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAATCAggg > 1:4038419/1‑68 (MQ=255) ttCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAggg > 1:3455867/1‑67 (MQ=255) ttCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTCGa < 1:1992200/71‑1 (MQ=255) cTTTACATTTGCCTTGTTTTATGAATGCTCCTGAAGAGGTGTATAACa < 1:1497852/48‑1 (MQ=255) cTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGata > 1:2331303/1‑71 (MQ=255) cTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGat < 1:2422721/70‑1 (MQ=255) aCATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATaa > 1:866477/1‑42 (MQ=255) aTTTGCCTTGTTTTATGAATGCTCCTGAAGACGTGTATAACATa > 1:2664875/1‑44 (MQ=255) tGCCTTGTTTTATGAATGCTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGaa > 1:3950567/1‑66 (MQ=255) tGCCTTGTTTTATGAATGCTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGa > 1:2777451/1‑65 (MQ=255) tGCCTTGTTTTATGAATGCTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGa > 1:2772103/1‑65 (MQ=255) tGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATaa > 1:2170685/1‑42 (MQ=255) gCCTTGTTTTATGAATGCTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATAtt > 1:957026/1‑71 (MQ=255) cTTGTTTTATGAATGCTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTg < 1:1404839/71‑1 (MQ=255) cTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTg < 1:2637007/71‑1 (MQ=255) gTTTTATGAATACTCCTGAAGAGGTGTATAACATaa > 1:3004387/1‑36 (MQ=255) gTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGaa > 1:3539625/1‑70 (MQ=255) gTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGaa > 1:4502182/1‑70 (MQ=255) ttttATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGAAATGAATATATTTGAACa < 1:4494516/71‑1 (MQ=255) ttATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGt > 1:2597106/1‑48 (MQ=255) ttATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACaaa > 1:32301/1‑71 (MQ=255) tATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAAc > 1:786505/1‑71 (MQ=255) aTGAATACTCCAGAAGAGGTGTATAACATAATGGTACAAGCAg > 1:1031496/1‑43 (MQ=255) aaTGCTCCTGAAGAGGTGTATAACATAATGGTACAAGCAggg < 1:4338702/42‑1 (MQ=255) aaTACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGaa < 1:2727637/52‑1 (MQ=255) aaTACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGaa < 1:163335/52‑1 (MQ=255) tACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAata > 1:944318/1‑52 (MQ=255) tACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCAc > 1:2610147/1‑70 (MQ=255) | TTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCAC > W3110S.gb/302102‑302239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |