| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 58,390 | C→T | 10.5% | intergenic (+211/‑84) | djlA → / → yabP | DnaJ‑like protein, membrane anchored/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 58,390 | 0 | C | T | 10.5% | 110.1 / 3.9 | 38 | intergenic (+211/‑84) | djlA/yabP | DnaJ‑like protein, membrane anchored/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (21/13); new base T (2/2); total (23/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGTTTTTAACTC > W3110S.gb/58321‑58456 | ttGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAaca < 1:2381915/71‑1 (MQ=255)ttGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAaca < 1:3510359/71‑1 (MQ=255)ttGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAaca < 1:3664906/71‑1 (MQ=255) gACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAacac < 1:1925845/70‑1 (MQ=255) cTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAATACATAcc > 1:429795/1‑71 (MQ=255) gCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAATACATACCt > 1:2889077/1‑70 (MQ=255) cTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTg > 1:776994/1‑70 (MQ=255) cTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTg < 1:2392706/70‑1 (MQ=255) cTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTg < 1:2206765/70‑1 (MQ=255) ttGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGc > 1:3917358/1‑70 (MQ=255) gagaGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGAt > 1:2102364/1‑71 (MQ=255) gagaGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGAt > 1:2441801/1‑71 (MQ=255) gagCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAATACATACCTGAATGAGCGAttt < 1:3689323/71‑1 (MQ=255) gCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGAttttt > 1:4455130/1‑71 (MQ=255) cTTTATGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGAttttt > 1:1923852/1‑70 (MQ=255) cTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTg < 1:3083932/71‑1 (MQ=255) ttACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTg > 1:4292876/1‑69 (MQ=255) ttACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGa > 1:4410753/1‑70 (MQ=255) tACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGaa < 1:4358865/70‑1 (MQ=255) acgacgAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAg > 1:3242843/1‑71 (MQ=255) aGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTAtatt < 1:995714/71‑1 (MQ=255) gAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTAtatttatt < 1:2484361/71‑1 (MQ=255) ttATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAg < 1:3096419/71‑1 (MQ=255) ttATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTCGAAAGTATATTTATTCAg < 1:1752070/71‑1 (MQ=255) atatTTTTACGTCTTAAAAATAAAAAACACATACCTGAAt > 1:1940537/1‑40 (MQ=255) atatTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGa < 1:407524/70‑1 (MQ=255) tatTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAAc > 1:2186329/1‑71 (MQ=255) atTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGacc > 1:880715/1‑68 (MQ=255) tttttACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAAcg < 1:1356733/70‑1 (MQ=255) aCGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGca < 1:2021676/69‑1 (MQ=255) aaaaataaaaaACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGCACGCGCATCATGAGtt > 1:1429775/1‑71 (MQ=255) aaaaataaaaaACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGca > 1:2131785/1‑62 (MQ=255) aaaaataaaaaACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGtt > 1:1348939/1‑71 (MQ=255) aaaaataaaaaACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGtt > 1:1741505/1‑71 (MQ=255) aaaataaaaaACACATACCTGAATGAGCGATTTTTGAAAg > 1:2023736/1‑40 (MQ=255) aaataaaaaACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGtttt < 1:3931467/71‑1 (MQ=255) aataaaaaATACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGttttt < 1:2519881/71‑1 (MQ=255) aataaaaaACACATACCTGAATGAGCGATTTTTGa > 1:199725/1‑35 (MQ=255) aataaaaaACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGtttt > 1:336250/1‑70 (MQ=255) taaaaaACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGTTTTTaa > 1:1798060/1‑71 (MQ=255) aaaaaaCACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGaa > 1:35457/1‑50 (MQ=255) aaaaCACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGTTTTTAACTc < 1:3120767/71‑1 (MQ=255) | TTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGTTTTTAACTC > W3110S.gb/58321‑58456 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |