| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 306,593 | T→C | 26.2% | N655S (AAC→AGC) | yagX ← | predicted aromatic compound dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 306,593 | 0 | T | C | 26.2% | 66.0 / 26.1 | 42 | N655S (AAC→AGC) | yagX | predicted aromatic compound dioxygenase |
| Reads supporting (aligned to +/- strand): ref base T (19/12); new base C (7/4); total (26/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATT > W3110S.gb/306528‑306663 | cgtcCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGCTTTTa > 1:3843670/1‑71 (MQ=255) gtgtTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGtttt > 1:2895877/1‑55 (MQ=255) gtgtTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTAccc < 1:1312427/60‑1 (MQ=255) gtTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGCTTTTACCCTg > 1:2221455/1‑60 (MQ=255) gtTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGCTTTTACCCTGCCAGCCGACGc > 1:2592565/1‑71 (MQ=255) ttGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCt > 1:1102436/1‑71 (MQ=255) ttGAATATCACACCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCt > 1:1320154/1‑71 (MQ=255) gAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGtttt > 1:2838998/1‑50 (MQ=255) gAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGtttt > 1:435342/1‑50 (MQ=255) gAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGa > 1:4256144/1‑65 (MQ=255) gAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGc > 1:852700/1‑71 (MQ=255) gAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGc > 1:1587267/1‑71 (MQ=255) aaTATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGc > 1:3297202/1‑70 (MQ=255) aaTATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGc < 1:2163701/70‑1 (MQ=255) atatCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGc < 1:155707/69‑1 (MQ=255) atatCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCa > 1:2259845/1‑71 (MQ=255) atatCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCa > 1:1318739/1‑71 (MQ=255) tatCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTAcc > 1:3895851/1‑51 (MQ=255) atCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGcc < 1:839421/59‑1 (MQ=255) tCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGCCAtt > 1:1952830/1‑70 (MQ=255) tCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGCCATTg > 1:2507876/1‑71 (MQ=255) aCCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGc > 1:4270568/1‑71 (MQ=255) aCCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGc > 1:3398662/1‑71 (MQ=255) ccccAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGtttt < 1:2993906/42‑1 (MQ=255) gCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGtt < 1:1197444/35‑1 (MQ=255) gCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGtt < 1:3444350/35‑1 (MQ=255) cGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAg > 1:3767010/1‑71 (MQ=255) ccATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGt < 1:2984227/71‑1 (MQ=255) ccATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGt < 1:3087651/71‑1 (MQ=255) tCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTAtt > 1:1140110/1‑71 (MQ=255) tCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGCCGGTCAAGTTGGTAtt < 1:2509039/71‑1 (MQ=255) tCCGCCCGCTGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTgg < 1:4528609/63‑1 (MQ=255) ccgcccgcTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACg > 1:2667903/1‑70 (MQ=255) cgcccgcTGGCAGCAATGTTTTTACCCTGCCAGCCGATGCTGCCATTGGCGGTCAAGTTGGTATTGACGTa < 1:2125504/71‑1 (MQ=255) cgcccgcTGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGc < 1:3411065/43‑1 (MQ=255) cgcccgcTGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGCCAtt > 1:3905872/1‑47 (MQ=255) gcccgcTGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAg < 1:3692781/71‑1 (MQ=255) ccgcTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTg > 1:2710775/1‑57 (MQ=255) cgcTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTAtt < 1:926423/61‑1 (MQ=255) tGGCAGCAATCTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTAtt < 1:452690/58‑1 (MQ=255) ggCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTa > 1:304410/1‑63 (MQ=255) cagcaATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGtt > 1:2978977/1‑49 (MQ=255) cagcaATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGtt > 1:2792217/1‑49 (MQ=255) caATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGa > 1:1309295/1‑54 (MQ=255) aaTGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTc < 1:3143230/63‑1 (MQ=255) aTGCTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTa > 1:439407/1‑48 (MQ=255) aTGCTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCt < 1:1332658/71‑1 (MQ=255) tGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTgg < 1:1759576/45‑1 (MQ=255) tttttACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTa > 1:310117/1‑69 (MQ=255) tttttACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTAtt < 1:4119076/71‑1 (MQ=255) | CGTCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATT > W3110S.gb/306528‑306663 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |