| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 338,000 | A→G | 12.0% | H151R (CAC→CGC) | yahG → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 338,000 | 0 | A | G | 12.0% | 124.8 / 9.8 | 50 | H151R (CAC→CGC) | yahG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (25/19); new base G (3/3); total (28/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
AAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAAC > W3110S.gb/337934‑338069 | aaGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCAc < 1:2520892/68‑1 (MQ=255) ggcTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATgg < 1:3598845/71‑1 (MQ=255) ggcTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATgg < 1:3131117/71‑1 (MQ=255) tGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCgg > 1:2593254/1‑71 (MQ=255) tGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCgg > 1:749936/1‑71 (MQ=255) gAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGa > 1:411710/1‑60 (MQ=255) gAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGa > 1:1640087/1‑60 (MQ=255) gAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGa > 1:2492152/1‑60 (MQ=255) aaCTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGa > 1:1036764/1‑49 (MQ=255) aCTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGgt < 1:3863250/70‑1 (MQ=255) ggcggcTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGAt > 1:3201730/1‑60 (MQ=255) ggcggcTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTa > 1:1359204/1‑71 (MQ=255) gcggcTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGtt < 1:1492580/69‑1 (MQ=255) cggcTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTAcc < 1:4243662/71‑1 (MQ=255) ttCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCgg > 1:131469/1‑71 (MQ=255) tCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCAc > 1:4189240/1‑36 (MQ=255) cGGGGAGATCACCTTCTCGCCGTGTCAAGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCt > 1:4605858/1‑71 (MQ=255) gggAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCg < 1:197680/71‑1 (MQ=255) gagaTCACCTTCTCGCCGTGTCACGAGCGCGACTGCGTg < 1:1408755/39‑1 (MQ=255) gagaTCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCTATGGCgg > 1:4138647/1‑52 (MQ=255) gaTCACCTTCTCGCCGTGTCACGAGCGCGACTGCGTggg > 1:3045210/1‑39 (MQ=255) gaTCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGt > 1:519990/1‑71 (MQ=255) aTCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGa < 1:3217196/43‑1 (MQ=255) aTCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATg < 1:365605/45‑1 (MQ=255) aTCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGtt < 1:2745427/71‑1 (MQ=255) tCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGtt < 1:2681560/53‑1 (MQ=255) tCACCTTCTCGCCGTGT‑ACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTa < 1:3265012/71‑1 (MQ=255) cACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTc > 1:2983871/1‑57 (MQ=255) aCCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATc > 1:4402399/1‑38 (MQ=255) aCCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGa > 1:2387896/1‑40 (MQ=255) cTTCTCGCCGTGTCACGAGCGCGACTGCGTGGGATCGATGGCGGGTGtt < 1:2141817/49‑1 (MQ=255) tctcGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGca > 1:1774186/1‑70 (MQ=255) tctcGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGca > 1:1651782/1‑70 (MQ=255) tctcGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGca > 1:3939465/1‑70 (MQ=255) ctcGCCGTGTCACGAGCACGACTGCGTGGGATCGa > 1:310447/1‑35 (MQ=255) cGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATc > 1:4028555/1‑71 (MQ=255) gCCGTGTAACGAGCGCGACTGCGTGGGATCGATGGCGGGTGTTACCt > 1:904832/1‑47 (MQ=39) ccGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCg < 1:124167/54‑1 (MQ=255) tCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTc < 1:1504624/48‑1 (MQ=255) tCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaaa < 1:1980459/71‑1 (MQ=255) cACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaaa < 1:1785599/70‑1 (MQ=255) aCGAGCGCGACTGCGTGGGATCGATGGCGGGTGTTAc < 1:634255/37‑1 (MQ=255) aCGAGCGCGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaaaa > 1:846763/1‑70 (MQ=255) aCGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCgg > 1:248004/1‑42 (MQ=255) cGAGCACTACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTAt < 1:275903/54‑1 (MQ=255) cGAGCACGACTGCGTGGGATCGATGGCGGGTGTTAc < 1:2729985/36‑1 (MQ=255) gAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaaca > 1:3872857/1‑70 (MQ=255) gAGCACGACTGCGTGGGATCGATGGCGGGTGTTACATCGGCCTCGATGTTTATGCACATCGTAAaaaaca > 1:2130335/1‑70 (MQ=255) aGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaacaaa < 1:4619149/71‑1 (MQ=255) cACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaacaaaac > 1:2677839/1‑71 (MQ=255) | AAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAAC > W3110S.gb/337934‑338069 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |