| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 338,985 | G→A | 19.6% | intergenic (+18/‑32) | yahG → / → yahH | conserved hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 338,985 | 0 | G | A | 19.6% | 102.9 / 18.5 | 46 | intergenic (+18/‑32) | yahG/yahH | conserved hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (16/21); new base A (6/3); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01 | |||||||||||
TGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGT > W3110S.gb/338916‑339048 | tGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGa > 1:3178700/1‑71 (MQ=255) gCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGAt > 1:3319722/1‑71 (MQ=255) ccTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGa < 1:1094914/69‑1 (MQ=255) ccTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGAt < 1:3571295/70‑1 (MQ=255) gCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGa > 1:343105/1‑66 (MQ=255) cGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATgcggcg < 1:355908/69‑1 (MQ=255) gagaAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTg > 1:1606689/1‑70 (MQ=255) gagaAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTg < 1:4196226/70‑1 (MQ=255) aaaGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATgcggc < 1:2885639/64‑1 (MQ=255) aaaGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACg > 1:1046795/1‑71 (MQ=255) aaGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGc < 1:2715722/71‑1 (MQ=255) aaGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGc < 1:966934/71‑1 (MQ=255) aGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGcc > 1:3086115/1‑71 (MQ=255) aGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGAATGCGGCGTGAACGcc > 1:2802019/1‑71 (MQ=255) ccATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCtt < 1:1377214/71‑1 (MQ=255) ccATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCtt < 1:3978488/71‑1 (MQ=255) ccATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCtt < 1:1894502/71‑1 (MQ=255) ttCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATgcggcg < 1:4543342/58‑1 (MQ=255) ttCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAAc < 1:4497057/63‑1 (MQ=255) ttCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATc < 1:2368279/71‑1 (MQ=255) ttCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGAATGCGGCGTGa < 1:1691404/61‑1 (MQ=255) cTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCg > 1:2339213/1‑71 (MQ=255) cTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCg > 1:1030980/1‑71 (MQ=255) gTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGaa > 1:3407648/1‑52 (MQ=255) aCGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGaa > 1:3610407/1‑46 (MQ=255) cGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTAt > 1:52706/1‑53 (MQ=255) ttACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGgcgc > 1:4296519/1‑69 (MQ=255) aCGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGaa > 1:548129/1‑41 (MQ=255) aCGGCGTCTGACCTACATGTTCATGCCGAATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAg > 1:3294173/1‑70 (MQ=255) cGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATc < 1:4610852/49‑1 (MQ=255) cGGCGTCTGACCTACATGTTCATGCCGAATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGa < 1:967651/70‑1 (MQ=255) ggCGTCTGACCTACATGTTCATGCCGAATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAAt < 1:1071273/71‑1 (MQ=255) gTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTg < 1:488100/71‑1 (MQ=255) tCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGa < 1:751418/65‑1 (MQ=255) tGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAg > 1:2116043/1‑71 (MQ=255) aCCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGc < 1:3271811/71‑1 (MQ=255) cTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAg < 1:3783959/67‑1 (MQ=255) aCATGTTCATGCCGAATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGc > 1:262360/1‑67 (MQ=255) aCATGTTCATGCCGAATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGa > 1:3214458/1‑71 (MQ=255) aCATGTTCATGCCGAATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGa > 1:208749/1‑71 (MQ=255) aCATGTTCATGCCGAATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCG‑AAGAATCTGTAGGCCTGa > 1:4183707/1‑70 (MQ=14) cATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGc > 1:2292852/1‑39 (MQ=37) cATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGa < 1:2571254/70‑1 (MQ=255) tGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATaa < 1:2536850/71‑1 (MQ=39) ttCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGc > 1:249879/1‑71 (MQ=35) aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGt < 1:2939614/70‑1 (MQ=31) | TGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGT > W3110S.gb/338916‑339048 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |