| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 342,943 | T→C | 19.4% | V279A (GTT→GCT) | yahK → | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 342,943 | 0 | T | C | 19.4% | 74.5 / 13.8 | 36 | V279A (GTT→GCT) | yahK | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (16/13); new base C (2/5); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGAAACTCAGGA > W3110S.gb/342873‑343012 | accTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGt < 1:3946772/71‑1 (MQ=255) cTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGctt > 1:1121088/1‑71 (MQ=255) tGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGtttt > 1:365708/1‑70 (MQ=255) gCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGCTTTc > 1:985682/1‑70 (MQ=255) cTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCa < 1:3531011/70‑1 (MQ=255) aaGCGTGATGGCACCATGACGCTGGTTGCTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCt > 1:3308004/1‑71 (MQ=255) gCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGctt < 1:3580624/62‑1 (MQ=255) gCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGctt < 1:2317442/62‑1 (MQ=255) ccATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGc < 1:586793/71‑1 (MQ=255) tGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCAt < 1:1326568/61‑1 (MQ=255) tGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCAt > 1:2199978/1‑61 (MQ=255) gACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTg > 1:143656/1‑71 (MQ=255) aCGCTGGTTGGTGCGCCTGCGACACCGCATAAATAGCCGGAAGTTTTCAAc > 1:3019873/1‑51 (MQ=255) aCGCTGGTTGGTGCGCCTGCGACACCGCATAAATAGCCGGAAGTTTTCAAc > 1:3014515/1‑51 (MQ=255) tgttggtGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAg > 1:4186916/2‑71 (MQ=255) ggttggtGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAg > 1:894977/1‑71 (MQ=255) gttggtGCGCCTGCGACACCGCATAAATCGCAGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAg < 1:2004180/70‑1 (MQ=255) tggtGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCg > 1:173936/1‑71 (MQ=255) ggtGCGCCTGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGAt < 1:584180/47‑1 (MQ=255) tgcgactgcgacACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGtt < 1:3178398/71‑1 (MQ=255) gcCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTa < 1:4400769/71‑1 (MQ=255) ccTGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCg < 1:695463/57‑1 (MQ=255) ccTGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCg < 1:3194114/57‑1 (MQ=255) cTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATg < 1:3128825/71‑1 (MQ=255) cGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGaaa > 1:199525/1‑44 (MQ=255) cGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGa < 1:2720658/69‑1 (MQ=255) acacCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTAt < 1:2036307/65‑1 (MQ=255) acCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGa > 1:3421686/1‑65 (MQ=255) ccGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTggcgg < 1:3098683/71‑1 (MQ=255) cGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGAtt > 1:941354/1‑65 (MQ=255) cGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGAtt > 1:2308137/1‑65 (MQ=255) cATAAATCGCCGGAAGTTTTCAACCTGATCATGaaa > 1:3395845/1‑36 (MQ=255) tAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTggcgg < 1:1601728/66‑1 (MQ=255) aaTCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTa > 1:1695169/1‑54 (MQ=255) aTCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTgg > 1:3070245/1‑60 (MQ=255) tCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAg > 1:1019191/1‑70 (MQ=255) cGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGa > 1:4013294/1‑70 (MQ=255) gCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGaa < 1:1917435/70‑1 (MQ=255) ccGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGaaa < 1:2932979/70‑1 (MQ=255) ggAAGTTTTCAACCTGATCTTGAAACGCCGTGCGATAGc < 1:4398377/39‑1 (MQ=255) gTTTTCAACTTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGAAACTCAGga < 1:2291802/71‑1 (MQ=255) ttttCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTggcgg < 1:3380759/52‑1 (MQ=255) ttttCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGAAACTc < 1:3951531/66‑1 (MQ=255) | ACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGAAACTCAGGA > W3110S.gb/342873‑343012 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |