| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 370,876 | C→T | 23.1% | G143G (GGC→GGT) | mhpC → | 2‑hydroxy‑6‑ketonona‑2,4‑dienedioic acid hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 370,876 | 0 | C | T | 23.1% | 47.9 / 10.2 | 26 | G143G (GGC→GGT) | mhpC | 2‑hydroxy‑6‑ketonona‑2,4‑dienedioic acid hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (11/9); new base T (4/2); total (15/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
GGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGC > W3110S.gb/370811‑370943 | ggCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGcggcgg < 1:2698736/71‑1 (MQ=255) aTAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTa < 1:4495840/69‑1 (MQ=255) aTAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACg > 1:2871882/1‑71 (MQ=255) ttCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGggc < 1:4282359/58‑1 (MQ=255) ttCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACg < 1:1385926/57‑1 (MQ=255) ttCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAgttt > 1:1007569/1‑71 (MQ=255) ccTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAgtttg < 1:2973359/67‑1 (MQ=255) aaTGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGTGGCGGTACGGGCGGCATGAGTTTGTTTACGcc < 1:1706621/70‑1 (MQ=255) aaTGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGcc > 1:1168719/1‑70 (MQ=255) tGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCg < 1:3529137/69‑1 (MQ=255) ggCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGTGGCGGTACGGGCGGCATGAGTTTGTTTACg > 1:1328301/1‑65 (MQ=255) ccGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGc > 1:3116128/1‑70 (MQ=255) ggAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAgtttgtt > 1:927123/1‑57 (MQ=255) gcgcGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCa > 1:2171558/1‑67 (MQ=255) tCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAgg > 1:441883/1‑70 (MQ=255) gCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAgtttgtt < 1:191726/46‑1 (MQ=255) gCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATg < 1:4001582/56‑1 (MQ=255) cAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAgtttg < 1:4409969/43‑1 (MQ=255) aaaCTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTAtt > 1:592401/1‑69 (MQ=255) aaCTGGTGCTGATGGGTGGCGGTACGGGCGGCATGAgtttgtt < 1:3409052/43‑1 (MQ=255) aaCTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAgtttgtt > 1:1037634/1‑43 (MQ=255) aCTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGc < 1:552037/71‑1 (MQ=255) aCTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGc > 1:775414/1‑71 (MQ=255) tGCTGATGGGCGGCGGTACGGGCGGCATGAgtttgtt < 1:3293827/37‑1 (MQ=255) gCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGa > 1:1953819/1‑44 (MQ=255) cTGATGGGTGGCGGTACGGGCGGCATGAGTTTGTTTa > 1:3196642/1‑37 (MQ=255) gggTGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGc > 1:4426067/1‑71 (MQ=255) gggTGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGc > 1:4431152/1‑71 (MQ=255) ggcggcGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGc < 1:1486293/70‑1 (MQ=255) | GGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAATCAGC > W3110S.gb/370811‑370943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |