| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 415,058 | T→C | 9.8% | E373E (GAA→GAG) | sbcD ← | exonuclease, dsDNA, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 415,058 | 0 | T | C | 9.8% | 160.8 / 8.9 | 61 | E373E (GAA→GAG) | sbcD | exonuclease, dsDNA, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (30/25); new base C (3/3); total (33/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
CGGCGAGGGTATGCAACGTCGTGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGGGTTTCACGCTGTTGGCTG > W3110S.gb/414991‑415127 | cGGCGAGGGTATGCAACGTCGTGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGttct < 1:249266/68‑1 (MQ=255) gAGGGTATGCAACGTCGTGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTcc < 1:2092339/67‑1 (MQ=255) gTATGCAACGTCGTGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGc > 1:776716/1‑68 (MQ=255) tGCAACGTCGTGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGttcttc < 1:4280059/59‑1 (MQ=255) tGCAACGTCGTGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGAc < 1:1777543/70‑1 (MQ=255) tGCAACGTCGTGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACg > 1:255916/1‑71 (MQ=255) aCGTCGTGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGttcttc < 1:1717493/55‑1 (MQ=255) aCGTCGTGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCa < 1:311108/63‑1 (MQ=255) aCGTCGTGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGa > 1:1031741/1‑71 (MQ=255) aCGTCGTGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGa > 1:2295547/1‑71 (MQ=255) aCGTCGTGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGa > 1:3737196/1‑71 (MQ=255) aCGTCGTGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCCTCCAGTGc < 1:2117386/61‑1 (MQ=255) cgtcgtGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGc < 1:569225/60‑1 (MQ=255) gtcgtGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGa > 1:900490/1‑63 (MQ=255) tcgtGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTg < 1:1186217/71‑1 (MQ=255) tcgtGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTg < 1:387370/71‑1 (MQ=255) gtggtgAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGaa > 1:2688962/1‑71 (MQ=255) tggtgAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCCTCCAGTGCCAGACg < 1:2874322/61‑1 (MQ=255) gtgAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGAtt > 1:3531855/1‑65 (MQ=255) gtgAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGAaca < 1:2402828/70‑1 (MQ=255) gtgAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCCTCCAGTGCCAGACGGc > 1:2735893/1‑61 (MQ=255) tgAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGttcttc < 1:1054423/46‑1 (MQ=255) gAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAg > 1:3630111/1‑48 (MQ=255) gAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGt > 1:1062339/1‑49 (MQ=255) gAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGt > 1:2002691/1‑49 (MQ=255) aaaaGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGAACACctc < 1:1467354/71‑1 (MQ=255) aaGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGttct < 1:2244569/40‑1 (MQ=255) aaGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGttct > 1:3108698/1‑40 (MQ=255) aaGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGaa > 1:309334/1‑63 (MQ=255) aaGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGaa > 1:303655/1‑63 (MQ=255) aGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTc < 1:3062252/71‑1 (MQ=255) aTGCTGCAGACGTTGCTGCTGCGATTCATCCAGttcttc < 1:233608/39‑1 (MQ=255) aTGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGaa > 1:1938098/1‑60 (MQ=255) aTGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCCTCCAGTGCCAGACGGCGATTGAACACCTCTTCGa > 1:4245890/1‑71 (MQ=255) aTGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCCTCCAGTGCCAGACGGCGATTGAACACCTCTTCGa > 1:4251066/1‑71 (MQ=255) gctgcAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGa > 1:2482205/1‑53 (MQ=255) gctgcAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTg < 1:4591081/56‑1 (MQ=255) gcAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCt > 1:909082/1‑70 (MQ=255) aGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCa < 1:1954865/40‑1 (MQ=255) aGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAg < 1:2123259/71‑1 (MQ=255) aGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAg < 1:4146320/71‑1 (MQ=255) aCGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAg < 1:2446301/39‑1 (MQ=255) cGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGAaca > 1:4035792/1‑52 (MQ=255) cGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGAaca > 1:3131069/1‑52 (MQ=255) gTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCg < 1:262301/71‑1 (MQ=255) gTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCg > 1:3365155/1‑71 (MQ=255) gctgctgcGATTCATCCAGTTCTTCCAGTGCCAGACg > 1:2384021/1‑37 (MQ=255) gctgctgcGATTCATCCAGTTCTTCCAGTGCCAGACGGc < 1:1036028/39‑1 (MQ=255) tgctgcGATTCATCCAGTTCTTCCAGTGCCAGACGGCGa > 1:648007/1‑39 (MQ=255) tgctgcGATTCATCCAGTTCTTCCAGTGCCAGACGGCGAttaa > 1:4094667/1‑41 (MQ=255) tgctgcGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTc < 1:931007/53‑1 (MQ=255) tgctgcGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTCAACAcc < 1:591837/48‑1 (MQ=255) ctgcGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAggg > 1:968297/1‑71 (MQ=255) gcGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTg < 1:909560/65‑1 (MQ=255) cGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGGGttt > 1:3819700/1‑71 (MQ=255) cGATTAATCCAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGGGttt > 1:1265156/1‑71 (MQ=255) cATCCAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGGGTTTCACGc > 1:4360699/1‑71 (MQ=255) aTCCAGTTCTTCCGGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGGGTTTCACGCt < 1:424811/71‑1 (MQ=255) tCCAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTc < 1:3380044/41‑1 (MQ=255) ccAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCtt > 1:1858587/1‑39 (MQ=255) ccAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTc > 1:3208731/1‑40 (MQ=255) ccAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGa > 1:2413127/1‑42 (MQ=255) cAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGGGttt > 1:699762/1‑62 (MQ=255) cAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGGGTTTCACGCTGtt < 1:4592364/71‑1 (MQ=255) cAGTTCCTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGGGTTTCACGCTGtt < 1:3109484/71‑1 (MQ=255) aGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGTGTTTCACGCTGTtg < 1:2359014/71‑1 (MQ=255) gTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTc < 1:1269452/37‑1 (MQ=255) gTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGGGTTTCACGCTGTtgg < 1:781603/71‑1 (MQ=255) ttcttcCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGGGTTTCACg < 1:628010/63‑1 (MQ=255) ttcctcCAGTGCCAGACGGCGATTGAACACCTCTTc < 1:1791653/36‑1 (MQ=255) tcttcCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAg > 1:2187677/1‑44 (MQ=255) cttcCAGTGCCAGACGGCGATTGAACACCTCTTCGa > 1:2410877/1‑36 (MQ=255) cttcCAGTGCCAGACGGCGATTGAACACCTCTTCGACGc > 1:3404925/1‑39 (MQ=255) cttcCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGa > 1:2149596/1‑51 (MQ=255) cttcCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGGGTTTCa > 1:390727/1‑59 (MQ=255) cttcCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGGGTTTCAc > 1:3594341/1‑60 (MQ=255) cttcCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGGGTTTCACGCTGTtggctg > 1:4583055/1‑71 (MQ=255) cttcCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGGGTTTCACGCTGTtggct < 1:1330875/70‑1 (MQ=255) cttcCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGGGTTTCACGCTGTtgg > 1:192486/1‑68 (MQ=255) cttcCAGTGCCAGACGGCGATTGAACACCACTTCGACGCTGAGTTCGCTGAGGGTTTCACGCTGTtggctg > 1:547791/1‑71 (MQ=255) | CGGCGAGGGTATGCAACGTCGTGGTGAAAAGATGCTGCAGACGTTGCTGCTGCGATTCATCCAGTTCTTCCAGTGCCAGACGGCGATTGAACACCTCTTCGACGCTGAGTTCGCTGAGGGTTTCACGCTGTTGGCTG > W3110S.gb/414991‑415127 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |