| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 501,525 | A→G | 18.2% | L313P (CTG→CCG) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 501,525 | 0 | A | G | 18.2% | 123.2 / 21.1 | 55 | L313P (CTG→CCG) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (25/20); new base G (6/4); total (31/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCC > W3110S.gb/501458‑501595 | tAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGa < 1:1847786/70‑1 (MQ=255) aTGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaata < 1:3155437/71‑1 (MQ=255) ccGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaataat > 1:4409878/1‑70 (MQ=255) cGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCg < 1:1841647/71‑1 (MQ=255) gTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGc < 1:3465102/71‑1 (MQ=255) cGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAg > 1:2193863/1‑71 (MQ=255) ggAGTTACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTc < 1:1911050/70‑1 (MQ=255) gTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGc > 1:968638/1‑69 (MQ=255) tGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAg < 1:902021/71‑1 (MQ=255) gACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAg < 1:1143768/70‑1 (MQ=255) actaaaCAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGc > 1:33958/4‑70 (MQ=255) aCCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaataa > 1:2789788/1‑53 (MQ=255) aCCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaa > 1:4165248/1‑50 (MQ=255) aCCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCa > 1:4632620/1‑59 (MQ=255) aCCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAg < 1:2232603/69‑1 (MQ=255) aCCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCa > 1:1910612/1‑71 (MQ=255) aCCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCa > 1:2239923/1‑71 (MQ=255) ccAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGa < 1:1853125/48‑1 (MQ=255) cAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCATCa > 1:4181784/1‑69 (MQ=255) aaaCAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACGGAATAATCGCCAGCGTCGc > 1:758607/1‑63 (MQ=255) aaaCAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCg < 1:3406202/59‑1 (MQ=255) aaaCAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTc < 1:2653161/61‑1 (MQ=255) aaCAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACGGaata < 1:1890369/48‑1 (MQ=255) aaCAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGc < 1:557600/71‑1 (MQ=255) aCAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACGGaat < 1:3347993/46‑1 (MQ=255) aCAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGc > 1:2779617/1‑70 (MQ=255) aCAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAAACGc > 1:400166/1‑52 (MQ=255) cAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaataat > 1:4275383/1‑48 (MQ=255) aGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACGGaataa > 1:752989/1‑46 (MQ=255) aGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACGGaataa > 1:4014274/1‑46 (MQ=255) aGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACGGaataa > 1:162221/1‑46 (MQ=255) tCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGa < 1:1120360/40‑1 (MQ=255) cGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCa > 1:2633545/1‑58 (MQ=255) cACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGc < 1:2957323/58‑1 (MQ=255) cACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAg < 1:449434/66‑1 (MQ=255) aGGAAAAATGCGGCTAACGACTTACCAAACGGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCt > 1:845477/1‑70 (MQ=255) aGGAAAAATGCGGCTAACGACTTACCAAACAGaataa > 1:2493915/1‑37 (MQ=255) aGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCt > 1:4277683/1‑70 (MQ=255) ggAAAAATGCGGCTAACGACTTACCAAACAGaataat > 1:2514467/1‑37 (MQ=255) ggAAAAATGCGGCTAACGACTTACCAAACAGaataat > 1:1583667/1‑37 (MQ=255) gAAAAATGCGGCTAACGACTTACCAAACGGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGaa < 1:3152401/71‑1 (MQ=255) gAAAAATGCGGCTAACGACTTACCAAACAGaataa > 1:3243790/1‑35 (MQ=255) aaaaTGCGGCTAACGACTTACCAAACAGAATAATCGCCAga > 1:2204403/1‑40 (MQ=255) aaaTGCGGCTAACGACTTACCAAACAGAATAATAGCCAGCGTCGCCAGCACt > 1:3001937/1‑52 (MQ=255) cGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAAt < 1:223229/70‑1 (MQ=255) gACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCtta < 1:121847/54‑3 (MQ=255) ttACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAAtt < 1:2645547/60‑1 (MQ=255) aCCAAACGGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAaac > 1:3237886/1‑70 (MQ=255) aCCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCgg > 1:1551589/1‑42 (MQ=255) aCCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGt > 1:2930234/1‑43 (MQ=255) aCCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAtac < 1:3746361/70‑1 (MQ=255) aCCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAaac < 1:2085906/70‑1 (MQ=255) ccAAACGGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAaacaa < 1:112891/71‑1 (MQ=255) ccAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAaaca > 1:2095597/1‑70 (MQ=255) cAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAaacaa > 1:4629791/1‑70 (MQ=255) aGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCa < 1:3029850/60‑1 (MQ=255) aGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATccc < 1:1850572/71‑1 (MQ=255) | TAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCC > W3110S.gb/501458‑501595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |