| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 516,807 | C→T | 9.8% | E233K (GAA→AAA) | ybbN ← | predicted thioredoxin domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 516,807 | 0 | C | T | 9.8% | 115.3 / 3.8 | 41 | E233K (GAA→AAA) | ybbN | predicted thioredoxin domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (18/18); new base T (2/2); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 | |||||||||||
TACGCGTCTGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCATCTTCTGGATTCTCCGC > W3110S.gb/516738‑516876 | tACGCGTCTGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTc < 1:3301722/70‑1 (MQ=255)tACGCGTCTGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTc < 1:4237379/70‑1 (MQ=255) gcgTCTGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTg < 1:3393829/71‑1 (MQ=255) cgTCTGACCGTCTGCGGCGGTGAGATCTTTACTCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTgc < 1:2153423/71‑1 (MQ=255) cgTCTGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTgc < 1:1362491/71‑1 (MQ=255) cgTCTGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTgc < 1:2069395/71‑1 (MQ=255) gcggcgGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATg > 1:3452081/1‑71 (MQ=255) ggTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGtt > 1:840644/1‑71 (MQ=255) agaTCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGc < 1:1617240/63‑1 (MQ=255) aTCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCAt > 1:4405253/1‑44 (MQ=255) cTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAgcgc > 1:2707027/1‑70 (MQ=255) tttACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCt < 1:3500116/53‑1 (MQ=255) tACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAg < 1:3587370/70‑1 (MQ=255) gATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTc < 1:2338703/71‑1 (MQ=255) aTGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAgcgc < 1:395246/60‑1 (MQ=255) tGCCCGAACAGCAACTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGc > 1:672196/1‑71 (MQ=255) tGCCCGAACAGCAACTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGc < 1:1177630/71‑1 (MQ=255) tGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGc > 1:4127482/1‑71 (MQ=255) cccGAACAGCAACTCCAGCGCCTCTTAATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGcc < 1:3777374/70‑1 (MQ=255) gAACAGCAACTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGt > 1:253838/1‑58 (MQ=255) gAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGcc < 1:3528330/67‑1 (MQ=255) aCAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCg > 1:3432061/1‑71 (MQ=255) aGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGc < 1:2620939/71‑1 (MQ=255) aaCTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCAtc > 1:3153580/1‑71 (MQ=255) cTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGt < 1:2716510/49‑1 (MQ=255) cTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCa < 1:3768267/67‑1 (MQ=255) cTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCAtc < 1:1966013/69‑1 (MQ=255) ccAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGtt > 1:1564614/1‑48 (MQ=255) ccAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCAtcttct > 1:3786693/1‑71 (MQ=255) ccAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCAtcttct > 1:1215267/1‑71 (MQ=255) ccAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCAtctt > 1:3345830/1‑69 (MQ=255) ccAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCAtc > 1:870894/1‑67 (MQ=255) ccAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCAtc > 1:3845161/1‑67 (MQ=255) ccAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCAt > 1:606795/1‑66 (MQ=255) ccAGCGCCACTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTg > 1:342875/1‑49 (MQ=255) cAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCATCTTCTg < 1:3557389/71‑1 (MQ=255) aGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCATCTTCTgg < 1:4180978/71‑1 (MQ=255) aGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCATCTTCTgg < 1:3412181/71‑1 (MQ=255) ccTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCATCTTCTg < 1:3877994/66‑1 (MQ=255) ctctTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCATCTTCTGGATtct > 1:1903028/1‑71 (MQ=255) ctctTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCATCTTCTGGATtct > 1:1897665/1‑71 (MQ=255) ctTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCg < 1:2835734/42‑1 (MQ=255) tCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGt > 1:880212/1‑41 (MQ=255) cATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGTGTCGCCAGTGCGGCATCTTCTGGATTCTCCGc > 1:1810134/1‑70 (MQ=255) | TACGCGTCTGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCATCTTCTGGATTCTCCGC > W3110S.gb/516738‑516876 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |