| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 517,941 | A→G | 23.8% | S145P (TCA→CCA) | ybbO ← | predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 517,941 | 0 | A | G | 23.8% | 73.7 / 22.3 | 42 | S145P (TCA→CCA) | ybbO | predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (17/15); new base G (6/4); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGC > W3110S.gb/517876‑518010 | cAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGtc < 1:4366666/70‑1 (MQ=255)cAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGtca > 1:4570666/1‑71 (MQ=255) gcgcATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGtcat < 1:4361566/70‑1 (MQ=255) cATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATcac > 1:3737313/1‑70 (MQ=255) tatTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGtc < 1:1007515/63‑1 (MQ=255) gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCtt > 1:645962/1‑70 (MQ=255) gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCtt < 1:2269572/70‑1 (MQ=255) cgcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCAc > 1:2708911/1‑71 (MQ=255) cgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGTCATCACaa > 1:686707/1‑57 (MQ=255) tAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGTCATCACAATACGCCCTTCACCGTg < 1:90826/71‑1 (MQ=255) tAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACaa > 1:1867059/1‑55 (MQ=255) cgcCACGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGTCATCACAATACGCCCTTCACCGTGCgg > 1:2381455/1‑70 (MQ=255) gcCACGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGTCATCACAATACGCCCTTCACCGTGCGGt < 1:1415530/70‑1 (MQ=255) ccACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTc < 1:275148/59‑1 (MQ=255) ccACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTc < 1:2668411/59‑1 (MQ=255) cACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGccc < 1:4277268/55‑1 (MQ=255) cACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTaa > 1:2360394/1‑70 (MQ=255) cACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTaa > 1:4193161/1‑70 (MQ=255) aCGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGTCATCACAATACGCCCTTCACCGTGCGGTAACa > 1:2834666/1‑71 (MQ=255) aCGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACaa > 1:563849/1‑47 (MQ=255) aCGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACaa > 1:630392/1‑47 (MQ=255) aCGACCCGGCGTGGAGATTAATCCCATAACCGATGATGTCATCACAAt > 1:2465047/1‑48 (MQ=255) cGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGTCATCACAATACGCCCTTCACCGTGCGGTAACAt > 1:4070924/1‑71 (MQ=255) aCCCGGCGTGGAGATTAATCCCATCACAGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATcg > 1:3550791/1‑71 (MQ=255) tGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTg < 1:3384101/52‑1 (MQ=255) tGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGt < 1:4053913/68‑1 (MQ=255) gagaTTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACa > 1:1957942/1‑70 (MQ=255) agaTTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATc < 1:1856960/59‑1 (MQ=255) agaTTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAgg > 1:2823680/1‑71 (MQ=255) agaTTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAgg > 1:2354435/1‑71 (MQ=255) aTTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTa < 1:782204/52‑1 (MQ=255) aTCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATg < 1:3282847/71‑1 (MQ=255) tCACCGATGGTGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCAt < 1:3791607/64‑1 (MQ=255) tCACCGATGATGTCATCACAATACGCCCTTCACCg > 1:2298960/1‑35 (MQ=255) tCACCGATGATGTCATCACAATACGCCCTTCACCGTg < 1:3313365/37‑1 (MQ=255) tCACCGATGATGTCATCACAATACGCCCTTCACCGTg < 1:223622/37‑1 (MQ=255) aCCGATGGTGTCATCACAATACGCCCTTCACCGTGCGGTaa > 1:3914417/1‑41 (MQ=255) aCCGATGGTGTCATCACAATACGCCCTTCACCGTGCGGTAACATcg > 1:2449003/1‑46 (MQ=255) aCCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACa > 1:1762941/1‑43 (MQ=255) aCCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTaa > 1:1111466/1‑53 (MQ=255) aCCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCt < 1:3953326/70‑1 (MQ=255) aCCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGCTGAGCt < 1:1695718/70‑1 (MQ=255) gatgatGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTg < 1:2193452/68‑1 (MQ=255) gatgatGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTg < 1:2193142/68‑1 (MQ=255) gatgatGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGgtg < 1:3156235/71‑1 (MQ=255) atgatgTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGgtgt > 1:2617613/1‑71 (MQ=255) gatgTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTgc > 1:212829/1‑71 (MQ=255) | CAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGC > W3110S.gb/517876‑518010 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |