| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 546,484 | G→T | 27.5% | G194V (GGC→GTC) | fdrA → | predicted acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 546,484 | 0 | G | T | 27.5% | 123.0 / 29.1 | 58 | G194V (GGC→GTC) | fdrA | predicted acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (22/20); new base T (9/7); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
GGGGCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTGGCA > W3110S.gb/546416‑546551 | ggggCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGc > 1:3385653/1‑70 (MQ=255) gggCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCaa > 1:110816/1‑71 (MQ=255) gggCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCaa > 1:2697521/1‑71 (MQ=255) ccGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAatat > 1:4320698/1‑71 (MQ=255) ggACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATAtt < 1:1281850/70‑1 (MQ=255) ggACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATAtt < 1:3177212/70‑1 (MQ=255) aCTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGc < 1:3107056/71‑1 (MQ=255) aCTGAGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGc > 1:3453102/1‑71 (MQ=255) ggTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCAt < 1:1068181/71‑1 (MQ=255) gTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCAtt < 1:1355502/71‑1 (MQ=255) tACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTg < 1:2164361/71‑1 (MQ=255) aCGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTgg > 1:2547745/1‑71 (MQ=255) cGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGgc > 1:2335188/1‑71 (MQ=255) tCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCAt > 1:1002127/1‑65 (MQ=255) cGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCt > 1:900321/1‑71 (MQ=255) cGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCt > 1:52667/1‑71 (MQ=255) gatgatTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCtt > 1:1054116/1‑71 (MQ=255) gatgatTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCtt > 1:2056196/1‑71 (MQ=255) atTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCgg < 1:2174842/71‑1 (MQ=255) ttGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGt < 1:1424265/71‑1 (MQ=255) ttGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTg > 1:3869234/1‑60 (MQ=255) ttGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTg > 1:1156363/1‑60 (MQ=255) gCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTAc > 1:1116485/1‑71 (MQ=255) ggCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCgg < 1:2475191/71‑1 (MQ=255) ggCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCgg > 1:4093729/1‑71 (MQ=255) cacaCCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATT‑GCGCTTcc < 1:501204/60‑1 (MQ=255) acacCGCTGGCTTTTGCTAACGTGATGCCGGAAGGc > 1:2512085/1‑36 (MQ=255) cacCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAat < 1:3573174/38‑1 (MQ=255) acCGCTGGCTTTTGCTAACGTGATGCCTGAAGGCAATATTGGCGTCATTGGCGCTTCCGGt < 1:1134241/61‑1 (MQ=255) acCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAata < 1:2543556/38‑1 (MQ=255) ccGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAatat > 1:2153387/1‑38 (MQ=255) ccGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGAtt > 1:814236/1‑69 (MQ=255) cGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAg > 1:1994098/1‑71 (MQ=255) cGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAg > 1:3820519/1‑71 (MQ=255) gCTGGCTTTTGCTAATGTGATGCCGGAAGGCAata > 1:2553682/1‑35 (MQ=255) tGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGt < 1:2747223/56‑1 (MQ=255) tGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAg > 1:3669981/1‑71 (MQ=255) tGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGGCATTGGCGCTTCCGGt < 1:2752392/56‑1 (MQ=255) gCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTAc > 1:1637088/1‑56 (MQ=255) gCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAg < 1:1462263/69‑1 (MQ=255) gCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAg < 1:1707451/69‑1 (MQ=255) cTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCtg < 1:2608597/71‑1 (MQ=255) ttGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCtg < 1:3672948/68‑1 (MQ=255) gCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCtt > 1:2777879/1‑43 (MQ=255) gCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTcc < 1:3493661/45‑1 (MQ=255) gCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTc > 1:764853/1‑71 (MQ=255) aCGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCg > 1:4264197/1‑68 (MQ=255) cGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGa < 1:494389/71‑1 (MQ=255) gTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTc > 1:2695782/1‑65 (MQ=255) tGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTc < 1:310171/64‑1 (MQ=255) aTGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCTGTACCGGGATTCAGGAGCTGTGTTCGCAGATTgc > 1:1138828/1‑71 (MQ=255) tGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTgcg < 1:2431813/71‑1 (MQ=255) gCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTgcg > 1:1479117/1‑70 (MQ=255) ggAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTgg < 1:3356516/71‑1 (MQ=255) ggAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTgg < 1:2984389/71‑1 (MQ=255) ggAAGGCAATATTGGAGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTgg > 1:1306368/1‑71 (MQ=255) gAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTGGc < 1:3449045/71‑1 (MQ=255) aaGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTGGCa < 1:1725572/71‑1 (MQ=255) | GGGGCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTGGCA > W3110S.gb/546416‑546551 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |