| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,694 | A→G | 100% | pseudogene (114/261 nt) pseudogene (114/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,694 | 0 | A | G | 100.0% | 157.8 / NA | 48 | pseudogene (114/261 nt) pseudogene (114/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/21); total (27/21) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAG > W3110S.gb/547628‑547758 | tcgtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGc > 1:2062640/1‑70 (MQ=255)tcgtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGc > 1:2461714/1‑70 (MQ=255) gtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa < 1:706573/70‑1 (MQ=255) gtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa < 1:3842324/70‑1 (MQ=255) cgcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAacac < 1:3633795/71‑1 (MQ=255) cTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTg > 1:1408731/1‑71 (MQ=255) cacaCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCAc > 1:1315651/1‑71 (MQ=255) cacaCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCAc > 1:1410614/1‑71 (MQ=255) gATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:1195003/71‑1 (MQ=255) gATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:3107785/71‑1 (MQ=255) aTGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc > 1:3305109/1‑70 (MQ=255) aTGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc > 1:1055158/1‑70 (MQ=255) gtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAAt > 1:2329717/1‑71 (MQ=255) tgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATg < 1:127588/71‑1 (MQ=255) ggCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa > 1:3674058/1‑62 (MQ=255) gCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATg > 1:3252706/1‑63 (MQ=255) gCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTgg < 1:1168498/69‑1 (MQ=255) ttcttcACTTATCAGCGAACTAAACGAGGGCAAAaca > 1:3834075/1‑37 (MQ=255) cttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgc < 1:1239741/54‑1 (MQ=255) ttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg > 1:4088015/1‑67 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATg > 1:4026726/1‑56 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgag < 1:1862175/68‑1 (MQ=255) cTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGc < 1:1333093/61‑1 (MQ=255) cTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGc < 1:2966560/61‑1 (MQ=255) cTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGAc > 1:143898/1‑71 (MQ=255) aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCt < 1:2557508/55‑1 (MQ=255) aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga < 1:3621257/62‑1 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGcc > 1:1483586/1‑38 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGc > 1:928124/1‑37 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:4318715/46‑1 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:2705405/46‑1 (MQ=255) acAGCGAACTAAACGAGGGCAAAACACTCCTTCACGCCGTGCCGCCAATGCGCTGGCAGGAGATGACCGGa > 1:3335291/2‑71 (MQ=255) cAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCa > 1:4382558/1‑57 (MQ=255) cAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAc > 1:1743081/1‑71 (MQ=255) aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAcc < 1:104269/71‑1 (MQ=255) gCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa > 1:900296/1‑45 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATg > 1:1944688/1‑70 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATg > 1:1819683/1‑70 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa < 1:3694339/71‑1 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa < 1:3922073/71‑1 (MQ=255) cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTgg < 1:3820401/48‑1 (MQ=255) cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa > 1:1166911/1‑70 (MQ=255) tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa > 1:2597600/1‑69 (MQ=255) aaaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg < 1:2177998/49‑1 (MQ=255) aaaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGAc > 1:1930295/1‑57 (MQ=255) aaaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAg > 1:228020/1‑71 (MQ=255) aaaCAAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgaga > 1:3458186/1‑53 (MQ=39) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGaaa < 1:1654360/69‑1 (MQ=255) | TCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAG > W3110S.gb/547628‑547758 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |