| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 70,581 | C→T | 92.5% | V65V (GTC→GTT) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 70,581 | 0 | C | T | 92.5% | 1435.2 / 82.7 | 512 | V65V (GTC→GTT) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (18/20); new base T (243/230); total (262/250) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
Only 100 of 559 total aligned reads displayed.
AAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGG > W3110S.gb/70513‑70648 | aaaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAAtttgtttg < 1:886132/71‑1 (MQ=255)aaaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAAtttgtttg > 1:1950180/1‑71 (MQ=255)aaaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAAtttgtttg > 1:1608644/1‑71 (MQ=255) ttATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAc > 1:247418/1‑71 (MQ=255) tatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCgg > 1:108775/1‑71 (MQ=255) atTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCggg > 1:205704/1‑71 (MQ=255) atTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCggg > 1:4467357/1‑71 (MQ=255) ctcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTg < 1:3326105/70‑1 (MQ=255) ctcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGa > 1:2994514/1‑71 (MQ=255) ctcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGa > 1:3512207/1‑71 (MQ=255) cAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGata > 1:284107/1‑71 (MQ=255) aaTCTCACCATTCGCTGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGa < 1:3551000/68‑1 (MQ=255) aaTCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGatat < 1:2348664/71‑1 (MQ=255) aaTCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGatat < 1:3047285/71‑1 (MQ=255) aTCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAcc < 1:3628315/61‑1 (MQ=255) tctcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAttt > 1:4135780/1‑71 (MQ=255) tcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAttt > 1:3193327/1‑69 (MQ=255) cACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCg < 1:414583/58‑1 (MQ=255) cACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc > 1:1627025/1‑71 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAtttt < 1:2133989/68‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAttt < 1:1910484/67‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc < 1:4602520/70‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc < 1:470926/70‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCt > 1:2721142/1‑71 (MQ=255) ccATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGcc < 1:3934982/51‑1 (MQ=255) ccATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTg < 1:90898/71‑1 (MQ=255) aTTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGtt < 1:3793574/71‑1 (MQ=255) cgcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAAtttgtttg > 1:3874710/1‑44 (MQ=255) cgcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTcc > 1:2788970/1‑70 (MQ=255) ggTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:2262109/71‑1 (MQ=255) ggTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:2552879/71‑1 (MQ=255) gTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGt < 1:4402361/50‑1 (MQ=255) gaGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc < 1:558201/57‑1 (MQ=255) cAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTcc > 1:3241584/1‑64 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCt > 1:3129970/1‑58 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:4232201/1‑69 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:969856/1‑69 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:1410163/1‑69 (MQ=255) ggggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga > 1:3274629/1‑69 (MQ=255) gggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAcc > 1:2097854/1‑40 (MQ=255) gggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagag < 1:2507187/71‑1 (MQ=255) ggtggtGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagaga < 1:2322427/71‑1 (MQ=255) gtggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGat < 1:1208004/45‑1 (MQ=255) gtggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc > 1:71303/1‑52 (MQ=255) gtggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAt < 1:3486498/71‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGAc < 1:4199315/36‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGata < 1:4049947/45‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt < 1:129408/71‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGatat < 1:1511443/44‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAtt > 1:2183179/1‑45 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca > 1:2594565/1‑71 (MQ=255) gtgAAAAATCAGGGAAGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa > 1:4506130/1‑60 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:2687952/71‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:295369/71‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:1450704/71‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtc < 1:1768903/69‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGACGACCGGGTGATATTTTGCTGTTCCCGcc < 1:1364856/58‑1 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:1167732/57‑1 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat > 1:4577294/1‑70 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCTGACCGGGTGatat > 1:2635887/1‑41 (MQ=38) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGt < 1:1259126/36‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGatat > 1:1577784/1‑41 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTccc > 1:1862066/1‑53 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:3667837/1‑71 (MQ=255) aaaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAgg > 1:609977/1‑59 (MQ=255) aaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc < 1:3459798/71‑1 (MQ=255) aaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc > 1:2750181/1‑71 (MQ=255) aaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGata > 1:2884732/1‑38 (MQ=255) aaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTg > 1:3728105/1‑43 (MQ=255) aaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTc > 1:4081213/1‑49 (MQ=255) aaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACt < 1:86025/71‑1 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTccc > 1:3412024/1‑49 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:689155/52‑1 (MQ=255) aTCAGGGACGAGAATATGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTAc > 1:198247/1‑71 (MQ=255) tCAGTGACGAGAATTTGTTTGCCGACCGGGTGATAtttt < 1:496908/39‑1 (MQ=37) tCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagag > 1:4094683/1‑58 (MQ=255) tCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga > 1:1822170/1‑57 (MQ=255) tCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACg > 1:2571996/1‑71 (MQ=255) aCGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagag < 1:4368668/52‑1 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGt < 1:4259433/37‑1 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:4341262/44‑1 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagaga > 1:1792483/1‑52 (MQ=255) gagaATTTGTTTGCCGACCGGGTGATATTTTGCTGtt < 1:1062479/37‑1 (MQ=255) gagaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCAt > 1:2536269/1‑71 (MQ=255) agaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATc < 1:823618/71‑1 (MQ=255) agaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATc < 1:4427017/71‑1 (MQ=255) gaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATcc > 1:3581413/1‑71 (MQ=255) gaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATcc > 1:1127844/1‑71 (MQ=255) gaATTTGTTTGACGACCGGGTGATATTTTGGTGTTCCGGcc < 1:3974841/41‑1 (MQ=25) aaTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg < 1:3898738/42‑1 (MQ=255) aaTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg > 1:2399719/1‑71 (MQ=255) aaTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg < 1:4286152/71‑1 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCgg < 1:2925777/71‑1 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg < 1:1001512/70‑1 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAt > 1:3827079/1‑47 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAt < 1:1712760/47‑1 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:201184/1‑53 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg > 1:777124/1‑69 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg > 1:1325661/1‑69 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg > 1:4170275/1‑69 (MQ=255) | AAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGG > W3110S.gb/70513‑70648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |