| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 571,256 | A→G | 21.4% | T194A (ACA→GCA) | ybcM → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 571,256 | 0 | A | G | 21.4% | 141.7 / 35.7 | 70 | T194A (ACA→GCA) | ybcM | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (31/24); new base G (5/10); total (36/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGCTATCCGAACCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGACGATGTGAGGTTAAATAA > W3110S.gb/571187‑571326 | ctgctATCCGAACCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCta < 1:1430326/70‑1 (MQ=255)ctgctATCCGAACCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCta < 1:3571743/70‑1 (MQ=255) ctATCCGAACCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtgcat < 1:190752/70‑1 (MQ=255) aTCCGAACCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacatta > 1:958767/1‑70 (MQ=255) tCCGAACCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacattac < 1:2229949/70‑1 (MQ=255) tCCGAACACGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacattac < 1:2732700/70‑1 (MQ=255) cGAACCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATc > 1:1458243/1‑71 (MQ=255) aCCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtgca > 1:2341005/1‑61 (MQ=255) aCCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTGCATTACATCGcc > 1:1290360/1‑71 (MQ=255) ggCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCt < 1:2052440/71‑1 (MQ=255) gCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacat < 1:2453276/57‑1 (MQ=255) cacaAAATGGACAGCAAACTAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCg < 1:724799/63‑1 (MQ=255) acaAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacattac < 1:4280860/58‑1 (MQ=255) acaAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacat < 1:2892081/55‑1 (MQ=255) acaAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAg > 1:343371/1‑70 (MQ=255) caAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTGCATTACATc < 1:1177075/60‑1 (MQ=255) caAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGcc > 1:3432677/1‑63 (MQ=255) aaaTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAg > 1:2371494/1‑71 (MQ=255) aaaTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTa > 1:317659/1‑66 (MQ=255) aaaTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCaa > 1:3477542/1‑70 (MQ=255) aaaTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAg > 1:1412977/1‑71 (MQ=255) aaTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGc < 1:4426142/71‑1 (MQ=255) aaTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGc < 1:844771/71‑1 (MQ=255) aaTGGACAGCAAACAAGGTTGCTCGATATCTATACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGc > 1:571871/1‑71 (MQ=255) aTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAGCg < 1:4511547/71‑1 (MQ=255) aTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCg < 1:2815675/71‑1 (MQ=255) aTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCg < 1:1925089/71‑1 (MQ=255) gACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacat < 1:453884/47‑1 (MQ=255) gACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAg < 1:1952635/62‑1 (MQ=255) gACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAgg < 1:2292861/71‑1 (MQ=255) aCAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacatt < 1:489821/47‑1 (MQ=255) gCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGcc < 1:1384268/52‑1 (MQ=255) gCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAggggg > 1:1240268/1‑70 (MQ=255) cAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtgcattaca < 1:1300094/46‑1 (MQ=255) cAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacatta > 1:1596114/1‑44 (MQ=255) cAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacatta > 1:2922384/1‑44 (MQ=255) cAAACAAGGGTGCTCGATATCTCTACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAGc < 1:784762/62‑1 (MQ=255) aaCAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATc > 1:254766/1‑46 (MQ=255) aaCAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAgg > 1:3937654/1‑64 (MQ=255) aaCAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAg > 1:169874/1‑71 (MQ=255) aCAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGc < 1:4400593/59‑1 (MQ=255) aCAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCg < 1:1017532/60‑1 (MQ=255) aCAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGt < 1:1561389/71‑1 (MQ=255) cAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAg > 1:3643905/1‑57 (MQ=255) cAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAg > 1:660547/1‑57 (MQ=255) cAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAg > 1:665964/1‑57 (MQ=255) cAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGtt < 1:4065197/71‑1 (MQ=255) cAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGtt > 1:1455605/1‑71 (MQ=255) aaGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCg > 1:1214813/1‑58 (MQ=255) aGGTTGCTCGATATCTCTACATTTCTGTTTCtgcattaca > 1:574003/1‑40 (MQ=255) aGGTTGCTCGATATCTCTACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTc > 1:1003719/1‑71 (MQ=255) ttGCTCGATATCTCTACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCaa < 1:1572608/71‑1 (MQ=255) ttGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGcc > 1:3443123/1‑42 (MQ=255) tGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCa > 1:4159031/1‑69 (MQ=255) gCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCaaa < 1:1834328/70‑1 (MQ=255) cTCGATATCTCTACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAg < 1:2022369/70‑1 (MQ=255) cGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGtt > 1:1920804/1‑69 (MQ=255) tatCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAg < 1:1559010/48‑1 (MQ=255) tatCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACt > 1:1872640/1‑71 (MQ=255) atCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGc > 1:4120693/1‑40 (MQ=255) tctcTACATTTCTGTTTCTGCATTACATCGCCGTCTAGc < 1:4257072/39‑1 (MQ=255) tctcTACATTTCTGTTTCTGCATTACATCGCCGTCTAGc < 1:1930868/39‑1 (MQ=255) tctcTACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTg < 1:4503973/70‑1 (MQ=255) tctcTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCg < 1:2373913/44‑1 (MQ=255) tctcTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTg < 1:956742/70‑1 (MQ=255) ctctACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGa > 1:971645/1‑71 (MQ=255) tctACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAGCGa > 1:2864823/1‑43 (MQ=255) tctACATTTCTGTTTCTACATTACATCGCCGTCTAGCa < 1:808391/38‑1 (MQ=255) tctACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGAc > 1:3700129/1‑71 (MQ=255) tACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAGCGAg < 1:4077149/42‑1 (MQ=255) tACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAg > 1:3260086/1‑38 (MQ=255) tACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCg < 1:2377175/40‑1 (MQ=255) aCATTTCTGTTTCTACATTACATCGCCGTCTAGAAAGCGAGGGGGTAAGTTTCCAAAGta > 1:1256083/1‑60 (MQ=255) cATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAg > 1:1714413/1‑40 (MQ=255) cATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGACGa < 1:4370908/69‑1 (MQ=255) tttctgtttctgCATTACATCGCCGTCTAGCAAGCg < 1:1328201/36‑1 (MQ=255) tttctgtttctgCATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGtt > 1:3478737/1‑48 (MQ=255) tttCTGTTTCTACATTACATCGCCGTCTAGCAAGc < 1:3861568/35‑1 (MQ=255) tttCTGTTTCTACATTACATCGCCGTCTAGCAAGc < 1:4548070/35‑1 (MQ=255) tttCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGACGAtgt > 1:1466129/1‑70 (MQ=255) ttCTGTTTCTGCATTACATCGCCGTCTAGCAAGCg < 1:1413752/35‑1 (MQ=255) ttCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGta < 1:3054718/56‑1 (MQ=255) tCTGTTTCTACATTACATCGCCGTCTAGCAAGCGa > 1:183141/1‑35 (MQ=255) tCTGTTTCTACATTACATCGCCGTCTAGCAAGCGa > 1:3187085/1‑35 (MQ=255) tCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGACGATGTGAg > 1:3209842/1‑71 (MQ=255) cTGTTTCTGCATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATAc > 1:3600105/1‑57 (MQ=255) cTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGACGATGTGAgg > 1:1985428/1‑71 (MQ=255) tCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGACGATGTGAGGTTa < 1:3218828/69‑1 (MQ=255) acattacatCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGACGATGTGAGGTTAaataa > 1:1852721/1‑71 (MQ=255) | CTGCTATCCGAACCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGACGATGTGAGGTTAAATAA > W3110S.gb/571187‑571326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |