| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 579,625 | A→G | 15.4% | intergenic (+316/‑432) | ybcW → / → nohB | hypothetical protein/DNA packaging protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 579,625 | 0 | A | G | 15.4% | 57.3 / 5.2 | 26 | intergenic (+316/‑432) | ybcW/nohB | hypothetical protein/DNA packaging protein |
| Reads supporting (aligned to +/- strand): ref base A (10/12); new base G (2/2); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
TTAATTTGTTCAATCATTTGGTTAGGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCGTATTCAGTGTCG > W3110S.gb/579592‑579692 | ttAATTTGTTCAATCATTTGGTTAGGAAAGCGGGTGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGt > 1:3609839/1‑71 (MQ=21)ttAATTTGTTCAATCATTTGGTTAGGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAg > 1:4008106/1‑70 (MQ=33) tAATTTGTTCAATCATTTGGTTAGGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGt > 1:3075321/1‑70 (MQ=35) aaTTTGTTCAATCATTTGGTTAGGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGtt > 1:244274/1‑70 (MQ=35) aTTTGTTCAATCATTTGGTTAGGAAAGCGGGTGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGa < 1:69874/71‑1 (MQ=21) aTTTGTTCAATCATTTGGTTAGGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGa < 1:1814691/71‑1 (MQ=34) aTTTGTTCAATCATTTGGTTAGGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGa < 1:3822565/71‑1 (MQ=34) aTTTGTTCAATCATTTGGTTAGGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGa < 1:3370304/71‑1 (MQ=34) aTTTGTTCAATCATTTGGTTAGGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTATGTTCTTAGTTGa < 1:1549908/71‑1 (MQ=21) tCATTTGGTTAGGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAg < 1:4217046/67‑1 (MQ=35) aTTTGGTTAGGAAAGCGGGTGTTGCGGGTTGTTGTTATGCGGGTTCTGTTCTTAGTTGACATGAGGTTGcc > 1:3420153/1‑71 (MQ=255) aTTTGGTTAGGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGcc > 1:3787231/1‑71 (MQ=34) tttGGTTAGGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGtt < 1:3846624/56‑1 (MQ=35) ttGGTTAGGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGcccc > 1:510973/1‑71 (MQ=34) ttGGTTAGGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGcccc > 1:718340/1‑71 (MQ=34) ttAGGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGa < 1:1958522/53‑1 (MQ=35) ggAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCGTATTc < 1:3492317/70‑1 (MQ=35) aaaGCGGGTGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCGTATTCAgt < 1:2838814/71‑1 (MQ=21) aaaGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACAt < 1:2348923/51‑1 (MQ=35) aaaGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCGTATTCAgt > 1:4452499/1‑71 (MQ=34) aaGCGGATGTTGCGGGTTGTTGTTCTGAGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCGTATTCAgtg > 1:3342671/1‑71 (MQ=21) aGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGAc < 1:1994187/47‑1 (MQ=38) cGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGGCCCGTATTCAGTGTCg > 1:2748262/1‑71 (MQ=21) cGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCGTATTCAGTGTc > 1:3299730/1‑70 (MQ=35) ggATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTg < 1:3584358/42‑1 (MQ=38) gATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCGt < 1:251760/58‑1 (MQ=35) | TTAATTTGTTCAATCATTTGGTTAGGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTAGTTGACATGAGGTTGCCCCGTATTCAGTGTCG > W3110S.gb/579592‑579692 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |