| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 594,454 | T→C | 29.2% | K71K (AAA→AAG) | cusR ← | DNA‑binding response regulator in two‑component regulatory system with CusS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 594,454 | 0 | T | C | 29.2% | 103.7 / 56.3 | 72 | K71K (AAA→AAG) | cusR | DNA‑binding response regulator in two‑component regulatory system with CusS |
| Reads supporting (aligned to +/- strand): ref base T (28/23); new base C (6/15); total (34/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.80e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01 | |||||||||||
CCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGA > W3110S.gb/594386‑594521 | cccAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCttt < 1:3129275/71‑1 (MQ=255) ccAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTa < 1:3860675/71‑1 (MQ=255) ccAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTa < 1:1774397/71‑1 (MQ=255) gCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATAGGCATCCCTTTATTGGCGGAg > 1:4476252/1‑71 (MQ=255) ccTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCg > 1:1013216/1‑70 (MQ=255) cTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTa < 1:1388746/71‑1 (MQ=255) cTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTa < 1:1277756/71‑1 (MQ=255) tGACGCGATGTTCAATGGTGCCAAGCTCGGTAAGCAACAGAATCGGCATACCTTTATTGGCGGAGCg > 1:712568/1‑67 (MQ=255) gACGCGATGTTCAATGGTGCCAAGCGTGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACa > 1:2905294/1‑71 (MQ=255) gACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTaa < 1:1762425/69‑1 (MQ=255) gACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGt < 1:1442783/67‑1 (MQ=255) aCGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACAt < 1:153209/71‑1 (MQ=255) gcgATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCCTTATTGGCgg < 1:2228494/59‑1 (MQ=255) gATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATgcgc < 1:4135656/71‑1 (MQ=255) tGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCAc < 1:3107969/71‑1 (MQ=255) gTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCAc > 1:2609733/1‑70 (MQ=255) ttCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCgg > 1:2778647/1‑53 (MQ=255) ttCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACg < 1:2646887/70‑1 (MQ=255) ttCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACg < 1:4369192/70‑1 (MQ=255) ttCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATg < 1:2291468/64‑1 (MQ=255) ttCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGa > 1:4365382/1‑71 (MQ=255) ttCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCATTTATTGGCgg > 1:4354537/1‑53 (MQ=255) tCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGa > 1:848009/1‑70 (MQ=255) cAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATgcg > 1:1745217/1‑64 (MQ=255) cAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGata > 1:570766/1‑71 (MQ=255) cAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGata > 1:2677212/1‑71 (MQ=255) cAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGata > 1:4050137/1‑71 (MQ=255) aaTGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGata < 1:371327/70‑1 (MQ=255) aaTGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTa < 1:2810850/56‑1 (MQ=255) aTGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCg < 1:3656915/48‑1 (MQ=255) aTGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCg < 1:2108937/48‑1 (MQ=255) tGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCgg < 1:3828497/48‑1 (MQ=255) tGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATcc < 1:2143493/71‑1 (MQ=255) gTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGATATCCCa < 1:2542683/71‑1 (MQ=255) tGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAg < 1:1927719/71‑1 (MQ=255) ccAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGc < 1:3454753/70‑1 (MQ=255) cAAGCGCTGTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCg < 1:1473714/71‑1 (MQ=255) cAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCg > 1:2140384/1‑71 (MQ=255) aaGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTa > 1:524688/1‑47 (MQ=255) aaGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGt > 1:1211817/1‑71 (MQ=255) aaGCGCGGTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGGACGATATCCCAGCCGt > 1:1395827/1‑71 (MQ=255) aaGCGCGGTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGat > 1:2803555/1‑60 (MQ=255) aGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGtt < 1:246827/71‑1 (MQ=255) aGCGCGGTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGtt < 1:569304/71‑1 (MQ=255) agcgGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCa < 1:4254296/70‑1 (MQ=255) gTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTa > 1:1106814/1‑40 (MQ=255) gTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGt < 1:3525339/70‑1 (MQ=255) gTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTc < 1:2825381/71‑1 (MQ=255) aaGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGa > 1:1062739/1‑50 (MQ=255) aGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTaa > 1:4506381/1‑38 (MQ=255) aGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTc > 1:3561536/1‑68 (MQ=255) aGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTc > 1:874539/1‑68 (MQ=255) cAACAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGgca < 1:2617719/71‑1 (MQ=255) aaCAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATc > 1:2436160/1‑50 (MQ=255) cAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGgcag > 1:3678590/1‑69 (MQ=255) aGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCat < 1:2843939/71‑1 (MQ=255) aGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCat < 1:3539981/71‑1 (MQ=255) gAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCat < 1:2527122/70‑1 (MQ=255) aaTCGGCATCCCCTTATTGGCGGTGCGTAACATgcgc < 1:3380329/37‑1 (MQ=38) aaTCGGCATCCCCTTATTGGCGGAGCGTAACATgcgc < 1:2471263/37‑1 (MQ=255) ggCATCCCTTTATTGGCGGAGCGTAACATGCGCACGata > 1:842840/1‑39 (MQ=255) gCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCg < 1:517791/71‑1 (MQ=255) gCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCg < 1:2055859/71‑1 (MQ=255) aTCCCTTTATTGGCGGAGCGTAACATGCGCACGata > 1:3500648/1‑36 (MQ=255) aTCCCTTTATTGGCGGAGCGTAACATGCGCACGata > 1:1392483/1‑36 (MQ=255) aTCCCTTTATTGGCGGAGCGTAACATGCGCACGat > 1:2457563/1‑35 (MQ=255) aTCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAg < 1:896021/71‑1 (MQ=255) tCCCCTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGtt < 1:26536/46‑1 (MQ=255) ccccTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGa > 1:3563172/1‑71 (MQ=255) ccTTTATTGGCGGAGCGTAACATGCGCACGATATCCCa > 1:3111450/1‑38 (MQ=255) cTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCa > 1:480324/1‑45 (MQ=255) cTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCa > 1:1313969/1‑45 (MQ=255) cTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTc > 1:1854576/1‑49 (MQ=255) cTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTc > 1:3264868/1‑49 (MQ=255) tttATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTcc < 1:928/49‑1 (MQ=255) | CCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGA > W3110S.gb/594386‑594521 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |