| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 633,015 | G→T | 31.6% | V69V (GTG→GTT) | ybdL → | methionine aminotransferase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 633,015 | 0 | G | T | 31.6% | 112.9 / 37.7 | 60 | V69V (GTG→GTT) | ybdL | methionine aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (21/20); new base T (13/6); total (34/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
GCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCC > W3110S.gb/632945‑633078 | gcTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTg < 1:894956/71‑1 (MQ=255) aTTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAg > 1:1229910/1‑71 (MQ=255) tACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCa > 1:1124322/1‑67 (MQ=255) aCAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCt > 1:1020149/1‑71 (MQ=255) cAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCt < 1:3072407/70‑1 (MQ=255) gAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTTCAGGCCTTgcg > 1:2137562/1‑71 (MQ=255) aGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTTCAGGCCTTgcgc < 1:3785656/71‑1 (MQ=255) gCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTgcgc > 1:1018994/1‑70 (MQ=255) gCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAg < 1:3146440/70‑1 (MQ=255) cTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGc < 1:3760607/71‑1 (MQ=255) cTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGc < 1:42486/71‑1 (MQ=255) acGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTTCAGGCCTTGCGCGAGGCGa > 1:2807260/1‑63 (MQ=255) acGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCt > 1:3275943/1‑68 (MQ=255) cGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAg > 1:2138385/1‑70 (MQ=255) gTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAg < 1:1064093/45‑1 (MQ=255) gTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGaa < 1:778891/71‑1 (MQ=255) gTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGa > 1:505950/1‑70 (MQ=255) ttGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTTCAgg < 1:2914609/45‑1 (MQ=255) ttGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTTCAGGCCTTg > 1:3605104/1‑50 (MQ=255) ttGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGaa > 1:4445618/1‑70 (MQ=255) ttGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGaa > 1:2252969/1‑70 (MQ=255) ttGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGaa > 1:33656/1‑70 (MQ=255) gCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGcc > 1:1023973/1‑45 (MQ=255) gCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGc < 1:1839174/56‑1 (MQ=255) gCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAAc > 1:2010491/1‑71 (MQ=255) cacaGGGGGCAAACCAATACGCGCCCATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACg > 1:3593696/1‑71 (MQ=255) cacaGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCt > 1:4013292/1‑45 (MQ=255) ggggCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGCAc > 1:1482792/1‑70 (MQ=255) ggggCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAAc > 1:1423159/1‑70 (MQ=255) gggCAAACCAATACGCGCCCATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGt < 1:3278364/71‑1 (MQ=255) gCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGa > 1:2161961/1‑49 (MQ=255) gCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGttt > 1:979486/1‑71 (MQ=255) cAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTg < 1:1837851/71‑1 (MQ=255) aaaCCAGTACGCGCCCATGACCGGCGTTCAGGCCTTGCGCGa > 1:594037/1‑42 (MQ=38) aaCCAATACGCGCCCATGACCGGCGTGCAGGCCTTg < 1:3909883/36‑1 (MQ=255) ccAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGaa > 1:3673996/1‑61 (MQ=255) tACGCGCCCATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGaa < 1:2546219/50‑1 (MQ=255) aCGCGCCCATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTAt > 1:2571525/1‑71 (MQ=255) aCGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGaaa < 1:2405420/50‑1 (MQ=255) cgcgCCCATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTAt > 1:3343577/1‑70 (MQ=255) cgcgCCCATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTAt > 1:794621/1‑70 (MQ=255) gcgcCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCa < 1:1519928/71‑1 (MQ=255) gcCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAAc < 1:4108826/71‑1 (MQ=255) cccATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTg < 1:1678606/57‑1 (MQ=255) cccATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTg < 1:2863953/57‑1 (MQ=255) cATGACCGGCGTTCAGGCCTTGGGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAAc > 1:897996/1‑68 (MQ=255) aTGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGtt < 1:4271405/52‑1 (MQ=255) tGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACgg < 1:1983793/45‑1 (MQ=255) aCCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGaaaa > 1:3620999/1‑40 (MQ=255) aCCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTa > 1:3192227/1‑53 (MQ=255) aCCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGaa > 1:2566715/1‑45 (MQ=255) aCCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGa > 1:2153483/1‑68 (MQ=255) aCCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGc > 1:2076111/1‑71 (MQ=255) aCCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGc > 1:2193421/1‑71 (MQ=255) aCCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACAAGATg < 1:2335409/70‑1 (MQ=255) ccGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACgg < 1:3200384/42‑1 (MQ=255) ccGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGcc < 1:1627357/71‑1 (MQ=255) cGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTa > 1:1966963/1‑51 (MQ=255) cGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCa < 1:2142745/64‑1 (MQ=255) cGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGcc < 1:4157969/70‑1 (MQ=255) tGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGa < 1:3966019/37‑1 (MQ=255) | GCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCC > W3110S.gb/632945‑633078 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |