Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 641,944 G→A 10.5% E212K (GAA→AAA)  ybdR → predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb641,9440GA10.5% 163.1 / 8.7 57E212K (GAA→AAA) ybdRpredicted oxidoreductase, Zn‑dependent and NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (34/17);  new base A (3/3);  total (37/20)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

GCGTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGCGCGATCCC  >  W3110S.gb/641876‑642014
                                                                    |                                                                       
gCGTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTG‑‑AATCGCCTGTGCACGGTTGCTCGGTGCGGaa                                                                       >  1:834571/1‑69 (MQ=255)
     gCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGAt                                                                 >  1:1294694/1‑71 (MQ=255)
      cAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGAtt                                                                <  1:2749490/71‑1 (MQ=255)
        gTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGAtttt                                                              <  1:4237965/71‑1 (MQ=255)
          cTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGAt                                                                 >  1:1022839/1‑66 (MQ=255)
          cTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTttg                                                            >  1:1315176/1‑71 (MQ=255)
           tATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTttgt                                                           >  1:3549097/1‑71 (MQ=255)
             tGGTGCTGGTCCTGTGGGATTGTTGACAATCGGCTGTGCACGGTTGCTCGGTGCGGAACAGATTTttgttg                                                         >  1:3649273/1‑71 (MQ=255)
             tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTttgttg                                                         >  1:763097/1‑71 (MQ=255)
             tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTttgttg                                                         >  1:4198592/1‑71 (MQ=255)
               gTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTttgttgtt                                                       <  1:1531967/71‑1 (MQ=255)
               gTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTttgttgtt                                                       >  1:2836156/1‑71 (MQ=255)
                tGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTttgttg                                                         >  1:4520512/1‑68 (MQ=255)
                tGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTttgttg                                                         >  1:3915271/1‑68 (MQ=255)
                tGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTttgttg                                                         >  1:3331484/1‑68 (MQ=255)
                  cTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGat                                                    >  1:369327/1‑71 (MQ=255)
                     gTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGatcat                                                 <  1:2993120/71‑1 (MQ=255)
                       ccTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGatcatca                                               >  1:1620490/1‑71 (MQ=255)
                        cTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTttgttgtt                                                       >  1:4538896/1‑62 (MQ=255)
                          gtgGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGat                                                    >  1:971990/1‑63 (MQ=255)
                           tgGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGatc                                                   <  1:536220/63‑1 (MQ=255)
                           tgGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGATCATCATccc                                           <  1:3964651/71‑1 (MQ=255)
                           tgGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGATCATCATccc                                           >  1:3264874/1‑71 (MQ=255)
                            gggATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGAttttt                                                             >  1:358425/1‑52 (MQ=255)
                             ggATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGATCATCATCCCTa                                         >  1:2587647/1‑71 (MQ=255)
                                ttgttgACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTttgttgtt                                                       >  1:3169056/1‑54 (MQ=255)
                                  gttgACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTttgtt                                                          >  1:3497013/1‑49 (MQ=255)
                                  gttgACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGatcat                                                 >  1:872114/1‑58 (MQ=255)
                                       cAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCAt                               <  1:1144663/71‑1 (MQ=255)
                                        aaTCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCAtt                              <  1:161468/71‑1 (MQ=255)
                                         aTCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTg                                  <  1:3519296/66‑1 (MQ=255)
                                              cTGTGCACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGat                                                    >  1:2977873/1‑43 (MQ=255)
                                                 tgCACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCgccgcc                      >  1:2995586/1‑70 (MQ=255)
                                                   cACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGATCATCATCCCTAc                                        >  1:1232603/1‑50 (MQ=255)
                                                      ggTTGCTCGGTGCGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGc                <  1:803134/71‑1 (MQ=255)
                                                      ggTTGCTCGGTGCGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGc                >  1:774951/1‑71 (MQ=255)
                                                       gTTGCTCGGTGCGGAACAGATTTTTGTTGTTGatgatc                                                <  1:2542269/38‑1 (MQ=255)
                                                       gTTGCTCGGTGCGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCgccgcc                      >  1:2006572/1‑64 (MQ=255)
                                                        ttGCTCGGTGCGGAACAGATTTTTGTTGTTGatcatcat                                              <  1:35995/39‑1 (MQ=255)
                                                         tGCTCGGTGCGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTAc             >  1:2695961/1‑71 (MQ=255)
                                                          gCTCGGTGCGGAACAGATTTTTGTTGTTGATCATCATcc                                            >  1:1948438/1‑39 (MQ=255)
                                                            acgGTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttga                                      <  1:1374633‑M1/43‑16 (MQ=255)
                                                             cGGTGCGGAACAGATTTTTGTTGTTGATCATCATCCCTAc                                        >  1:1325224/1‑40 (MQ=255)
                                                             cGGTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttg                                       <  1:3242642‑M1/42‑15 (MQ=255)
                                                             cGGTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttg                                       <  1:3862677‑M1/42‑15 (MQ=255)
                                                              ggTGCGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGgcgc        >  1:3570034/1‑71 (MQ=255)
                                                              ggTGCGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGgcgc        >  1:1102884/1‑71 (MQ=255)
                                                              ggTGCGGAACAGATTTTTGTTGTTGATCATCATCCATACCGCTTGCATTTCGCCGCCGACCGCTACGgcgc        >  1:4182593/1‑71 (MQ=255)
                                                              ggTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaa                                     >  1:298811‑M1/1‑27 (MQ=255)
                                                              ggTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaa                                     >  1:108194‑M1/1‑27 (MQ=255)
                                                              ggTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaa                                     >  1:2943343‑M1/1‑27 (MQ=255)
                                                               gTGCGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGcgcg       <  1:197956/71‑1 (MQ=255)
                                                               gTGCGGAACAGATTTTTGTTGTTGATCATCACCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGcgcg       <  1:1433453/71‑1 (MQ=255)
                                                                tGCGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTg                                  <  1:938515/43‑1 (MQ=255)
                                                                 gCGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCgccgcc                      <  1:1007660/54‑1 (MQ=255)
                                                                  cGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGCGCGAt     <  1:3031495/70‑1 (MQ=255)
                                                                  cGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGCGCGATc    >  1:3239951/1‑71 (MQ=255)
                                                                    gAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTa              >  1:3758070/1‑59 (MQ=255)
                                                                    gAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGCGCGATccc  >  1:2433181/1‑71 (MQ=255)
                                                                    |                                                                       
GCGTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGATTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGCGCGATCCC  >  W3110S.gb/641876‑642014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: