| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 642,134 | A→G | 25.4% | E275G (GAA→GGA) | ybdR → | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 642,134 | 0 | A | G | 25.4% | 92.8 / 34.6 | 55 | E275G (GAA→GGA) | ybdR | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (19/22); new base G (6/8); total (25/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCAGGTCACCGGGGCGTGGATGCAGTAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGTTGCGTCAGTGTATTGCGGCGG > W3110S.gb/642066‑642202 | ggCAGGTCACCGGGGCGTGGATGCAGTAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGaaa < 1:3420465/71‑1 (MQ=255) gCAGGTCACCGGGGCGTGGATGCAGTAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAAc < 1:2406411/71‑1 (MQ=255) aCCGGGGCGTGGATGCAGTAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCt > 1:4608432/1‑70 (MQ=255) ggggCGTGGATGCAGTAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCTGAc > 1:1475637/1‑70 (MQ=255) cGTGGATGCAGTAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCTGACTa < 1:3154916/68‑1 (MQ=255) tGGATGCAGTAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGGAACgg < 1:227104/57‑1 (MQ=255) tGGATGCAGTAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCTGACTaa < 1:806993/67‑1 (MQ=255) tGGATGCAGTAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCTGACTa < 1:2897586/66‑1 (MQ=255) gATGCAGTAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGGAACGGTg > 1:3799138/1‑57 (MQ=255) gATGCAGTAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGGAACGGTg > 1:3568430/1‑57 (MQ=255) gATGCAGTAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCTGACTAACCTGa > 1:1953922/1‑70 (MQ=255) gTAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCTGACTAACCTGa < 1:3656397/64‑1 (MQ=255) tAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAAc < 1:485192/46‑1 (MQ=255) tAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAAc < 1:2430743/46‑1 (MQ=255) tAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAAc < 1:2377453/46‑1 (MQ=255) tAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACg < 1:2048427/47‑1 (MQ=255) aaTAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTg > 1:1954647/1‑49 (MQ=255) aTAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCt > 1:3267482/1‑50 (MQ=255) tAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCTGACTAACCTGAAACTGGAGGgc < 1:3558765/71‑1 (MQ=255) gACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGaa < 1:492598/39‑1 (MQ=255) gACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCTGa > 1:3292509/1‑49 (MQ=255) gACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCTGACTAACCTGAAACTGGAGGgca < 1:1761161/70‑1 (MQ=255) aCGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGGAACGGTGCTGACTAACCTGaa < 1:728328/58‑1 (MQ=255) aCGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGGAACGGTGCTGACTAACCTGaa < 1:774926/58‑1 (MQ=255) aCGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGGAACGGTGCTGACTAACCTGaa < 1:3397807/58‑1 (MQ=255) aCGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCTGACTAACCTGAAACTGGAGGgcag > 1:1809718/1‑70 (MQ=255) ccGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCagcagc < 1:3711100/71‑1 (MQ=255) gTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTg < 1:1233478/39‑1 (MQ=255) ggTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCTGACTAACCTGAAACTGGa > 1:2847389/1‑56 (MQ=255) ggTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTa > 1:3135718/1‑70 (MQ=255) ttttGAAGCGAAAGGCAGCACCACGGGAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAg < 1:3090476/71‑1 (MQ=255) ttttGAAGCGAAAGGCAGCACCACGGGAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCCGTAAAg < 1:3162752/71‑1 (MQ=255) ttGAAGCGAAAGGCAGCACCACGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCg > 1:45403/1‑71 (MQ=255) aaGGCAGCACCACGGGAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGTTGCGTCAg > 1:2057290/1‑71 (MQ=255) aaGGCAGCACCACGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGTTGCGTCa < 1:3370821/70‑1 (MQ=255) aaGGCAGCACCACGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGTTGCGTCAg < 1:2166364/71‑1 (MQ=255) gcagcaCCACGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGTTGCGTCAgtg < 1:1693189/70‑1 (MQ=255) gcagcaCCACGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGTTGCGTCAgtg > 1:4218331/1‑70 (MQ=255) agcaCCACGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGTTGCGTCAGTGTAt < 1:2976459/71‑1 (MQ=255) gcaCCACGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGc < 1:3584177/55‑1 (MQ=255) caccacGGGAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGTTGCg < 1:4195342/60‑1 (MQ=255) accacGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGt > 1:193254/1‑55 (MQ=255) ccacGGGAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGTTGCGTCAGTGTATTgcg > 1:1222005/1‑71 (MQ=255) cacGGGAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGTTGCGTCAgtg < 1:2177522/63‑1 (MQ=255) acGGGAACGGTGCTGACTAACCTGAAACTGGAGGgca > 1:3754647/1‑37 (MQ=255) acGGGAACGGTGCTGACTAACCTGAAACTGGAGGgca > 1:2315515/1‑37 (MQ=255) acGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGt > 1:4243834/1‑52 (MQ=255) acGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGt > 1:2707866/1‑52 (MQ=255) acGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGt > 1:3208006/1‑52 (MQ=255) acGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGt > 1:509271/1‑52 (MQ=255) cGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGt > 1:2382709/1‑51 (MQ=255) cGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGt > 1:3055588/1‑51 (MQ=255) cGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGTTGCGTCAGTGTATTgcggcg > 1:2722032/1‑71 (MQ=255) ggAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGTTGCGTCAGTGTATTGcggcgg < 1:2308609/71‑1 (MQ=255) ggAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGTTGCGTCAGTGTATTGcggcgg < 1:1714572/71‑1 (MQ=255) gAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGt < 1:1422370/42‑1 (MQ=255) gAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGt < 1:1290756/42‑1 (MQ=255) | GGCAGGTCACCGGGGCGTGGATGCAGTAATAGACGCCGTCGGTTTTGAAGCGAAAGGCAGCACCACGGAAACGGTGCTGACTAACCTGAAACTGGAGGGCAGCAGCGGTAAAGCGTTGCGTCAGTGTATTGCGGCGG > W3110S.gb/642066‑642202 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |