| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 664,084 | C→T | 16.0% | G101D (GGC→GAC) | dacA ← | D‑alanyl‑D‑alanine carboxypeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 664,084 | 0 | C | T | 16.0% | 140.7 / 18.0 | 57 | G101D (GGC→GAC) | dacA | D‑alanyl‑D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base C (20/27); new base T (5/4); total (26/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCA > W3110S.gb/664014‑664153 | cATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGc > 1:4157681/1‑71 (MQ=255)cATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGc > 1:1714465/1‑71 (MQ=255) cccGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGTCGa > 1:2751258/1‑70 (MQ=255) cccGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGa > 1:3988790/1‑70 (MQ=255) cccGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGAt < 1:601572/71‑1 (MQ=255) ttGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGa > 1:389635/1‑70 (MQ=255) ttGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGa > 1:4305140/1‑70 (MQ=255) aGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTaa < 1:2317783/71‑1 (MQ=255) cATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGTCGa < 1:1325394/56‑1 (MQ=255) cATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAg < 1:2855363/71‑1 (MQ=255) aTCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGt > 1:1465259/1‑71 (MQ=255) tCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTaa < 1:3956137/64‑1 (MQ=255) tCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAAt < 1:822751/66‑1 (MQ=255) aGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGttt < 1:1172034/70‑1 (MQ=255) aagaaCCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATc < 1:815875/61‑1 (MQ=255) aaCCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGAt < 1:1120439/46‑1 (MQ=255) aaCCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAAt > 1:2636778/1‑71 (MQ=255) aCCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAAtt < 1:1055156/71‑1 (MQ=255) ccTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAAttt < 1:3769857/71‑1 (MQ=255) cTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTa < 1:2561233/71‑1 (MQ=255) tttAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTaaa < 1:2068865/66‑1 (MQ=255) tAAACACCAGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGt < 1:3567698/43‑1 (MQ=255) acCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCttt < 1:1697162/71‑1 (MQ=255) ccGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTc < 1:3160079/71‑1 (MQ=255) cGGGTTACCGGTGGCCCATGCGTCGTTGTAGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCa < 1:4222142/71‑1 (MQ=255) cGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTc < 1:3891371/70‑1 (MQ=255) gTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAAttt < 1:3637887/57‑1 (MQ=255) ttACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATc < 1:2661065/41‑1 (MQ=255) tACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGt > 1:4185769/1‑43 (MQ=255) ccGGTGGCCCATGCGTCGTTGTCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTg > 1:4585773/1‑71 (MQ=255) ggTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCg < 1:3558651/55‑1 (MQ=255) ggTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGc > 1:2057550/1‑71 (MQ=255) gTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCttt < 1:1235505/59‑1 (MQ=255) gTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCa > 1:1023692/1‑61 (MQ=255) tGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGc > 1:3459825/1‑55 (MQ=255) cccATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATa < 1:3225865/71‑1 (MQ=255) ccATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTAc < 1:1915220/47‑1 (MQ=255) cATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAAtt < 1:2543018/43‑1 (MQ=255) cATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAAtt < 1:1728124/43‑1 (MQ=255) tGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGc < 1:846834/57‑1 (MQ=255) tGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGt < 1:1677735/71‑1 (MQ=255) gCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGt > 1:1093855/1‑70 (MQ=255) gCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGt > 1:2214707/1‑70 (MQ=255) cgtcgtTGTCGATAGTGACTAAATCAGTTTCTTTAAATTTACCg < 1:852324/44‑1 (MQ=255) cgtcgtTGTCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTa < 1:416168/70‑1 (MQ=255) cgtcgtTGCCGATAGTGACTAAATCAGTTTCTTTaa > 1:2103436/1‑36 (MQ=255) cgtcgtTGCCGATAGTGACTAAATCAGTTTCTTTAAAt > 1:4143548/1‑38 (MQ=255) cgtcgtTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCa > 1:4038601/1‑51 (MQ=255) cgtcgtTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCt > 1:1196154/1‑57 (MQ=255) cgtcgtTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTaa > 1:3286823/1‑71 (MQ=255) cgtcgtTGCCGATAGTGACTAAATAAGTTTCTTTAAAt > 1:3524128/1‑38 (MQ=255) cgtcgtTGCCGATAGTGAATAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTaa > 1:4507112/1‑71 (MQ=255) gtcgtTGTCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCAt > 1:3611793/1‑51 (MQ=255) gtcgtTGTCCATAGTGACTAAATCAGATTCTTTAAATTTAccc > 1:2053453/1‑42 (MQ=37) tcgtTGTCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGc > 1:1813856/1‑44 (MQ=255) gtTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCa > 1:160499/1‑47 (MQ=255) gtTGACGATAGTGACTAAATCAGTTTCTTTAAAttt > 1:211919/1‑36 (MQ=255) ttGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCttt < 1:1005844/44‑1 (MQ=255) gCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGtca > 1:3235725/1‑71 (MQ=255) | CATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCA > W3110S.gb/664014‑664153 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |