| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 682,913 | C→T | 17.1% | V301V (GTG→GTA) | hscC ← | Hsp70 family chaperone Hsc62, binds to RpoD and inhibits transcription |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 682,913 | 0 | C | T | 17.1% | 97.2 / 12.6 | 41 | V301V (GTG→GTA) | hscC | Hsp70 family chaperone Hsc62, binds to RpoD and inhibits transcription |
| Reads supporting (aligned to +/- strand): ref base C (19/15); new base T (5/2); total (24/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01 | |||||||||||
GTAATTTGCCAAACAGACGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTGTTCAATCGGCACTCGC > W3110S.gb/682846‑682979 | gTAATTTGCCAAACAGACGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCAc > 1:3970744/1‑70 (MQ=255) tAATTTGCCAAACAGACGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGTACCa < 1:3732631/71‑1 (MQ=255) tttGCCAAACAGACGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAg < 1:2938257/69‑1 (MQ=255) tttGCCAAACAGACGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAg < 1:2200073/69‑1 (MQ=255) tGCCAAACAGACGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACt < 1:3821534/70‑1 (MQ=255) tGCCAAACAGACGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACt < 1:3816295/70‑1 (MQ=255) cAAACAGACGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCg < 1:2073776/71‑1 (MQ=255) cGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACt > 1:1234636/1‑71 (MQ=255) cGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTc < 1:2711986/68‑1 (MQ=255) gCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCtt < 1:3917261/71‑1 (MQ=255) gCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCtt > 1:3397048/1‑71 (MQ=255) gATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCtt > 1:3161532/1‑69 (MQ=255) aTTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGTACCAGACTGTCGATTTGACTCGGCtt > 1:452445/1‑68 (MQ=255) tGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGAcgcg > 1:4214441/1‑70 (MQ=255) gCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGa > 1:999767/1‑65 (MQ=255) ccAGCGGCATTTGTGACGCGCCGCCAACCAGTACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATcg > 1:4338192/1‑71 (MQ=255) aGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATcgcg > 1:2927735/1‑71 (MQ=255) gCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATcgcg > 1:1890038/1‑70 (MQ=255) ggCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGAt > 1:3138363/1‑41 (MQ=255) tttGTGACGCGCCGCCAACCAGTACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGcc > 1:1072300/1‑71 (MQ=255) tttGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCtt < 1:2450339/49‑1 (MQ=255) tttGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCa > 1:223121/1‑51 (MQ=255) tttGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATcgc < 1:2201222/63‑1 (MQ=255) tgtgACGCGCCGCCAACCAGCACCAGACTGTCGATTTGa > 1:2683619/1‑39 (MQ=255) tgtgACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATcgcg > 1:4261972/1‑62 (MQ=255) tgtgACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCaa > 1:3842929/1‑65 (MQ=255) gtgACGCACCGCCAACCAGCACCACACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTg > 1:3427811/1‑70 (MQ=255) gACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCt > 1:721590/1‑43 (MQ=255) gACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCt > 1:1110564/1‑43 (MQ=255) aCGCGCCGCCAACCAGCACCAGGCTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTGTTCa < 1:3210963/71‑1 (MQ=255) aCGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTGTTCa < 1:2943518/71‑1 (MQ=255) aCGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTGTTCa < 1:1409705/71‑1 (MQ=255) aCGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCATGTTCa < 1:3752/71‑1 (MQ=255) cgccAACCAGTACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTGTTCAATCgg < 1:3078117/70‑1 (MQ=255) gccAACCAGTACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCa > 1:2283139/1‑46 (MQ=255) gccAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATcgcg < 1:4356771/51‑1 (MQ=255) cAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCa > 1:539884/1‑51 (MQ=255) aCCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCgcgc < 1:3494901/48‑1 (MQ=255) ccAGTACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTGTTCAATCGGCACTCg > 1:679803/1‑70 (MQ=255) cAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTGTTCAATCGGCACTCGc > 1:959724/1‑70 (MQ=255) aGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTGtt > 1:1556769/1‑55 (MQ=255) | GTAATTTGCCAAACAGACGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTGTTCAATCGGCACTCGC > W3110S.gb/682846‑682979 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |