| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 726,253 | A→G | 10.1% | R402R (CGT→CGC) | kdpB ← | potassium translocating ATPase, subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 726,253 | 0 | A | G | 10.1% | 182.3 / 11.0 | 69 | R402R (CGT→CGC) | kdpB | potassium translocating ATPase, subunit B |
| Reads supporting (aligned to +/- strand): ref base A (29/33); new base G (4/3); total (33/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
CGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCG > W3110S.gb/726185‑726323 | cgcgCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAGCATGgcggcg < 1:166174/71‑1 (MQ=255) gcgcAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGcgacga < 1:1529027/71‑1 (MQ=255) gcgcAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGcgacg > 1:994528/1‑70 (MQ=255) cgcAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGcgacg < 1:3182338/69‑1 (MQ=255) ccTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGaa > 1:3668138/1‑66 (MQ=255) ccTGATAGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCa > 1:908012/1‑71 (MQ=255) gATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATc > 1:2389451/1‑70 (MQ=255) aTCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATc < 1:1424627/69‑1 (MQ=255) aTCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATc < 1:782825/69‑1 (MQ=255) cTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGaa > 1:2688336/1‑71 (MQ=255) ttttGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATc < 1:1003958/62‑1 (MQ=255) tttGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCt > 1:29176/1‑71 (MQ=255) tttGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCa > 1:1521776/1‑59 (MQ=255) tttGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCt > 1:4028015/1‑71 (MQ=255) tGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCtt < 1:763258/70‑1 (MQ=255) aTCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGa < 1:4022131/63‑1 (MQ=255) aaCATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGa < 1:1547868/60‑1 (MQ=255) aaCATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGa < 1:3669580/60‑1 (MQ=255) aaCATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGa < 1:4585924/60‑1 (MQ=255) aCATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCtt < 1:87828/64‑1 (MQ=255) cATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAAc > 1:3116116/1‑60 (MQ=255) cATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAAc > 1:3767769/1‑60 (MQ=255) tCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAAt > 1:2671635/1‑44 (MQ=255) gTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCg < 1:2931149/71‑1 (MQ=255) tAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCgg < 1:1270417/71‑1 (MQ=255) gggAAGTGACCACCGTTAGCCTCAACATGGcgacg < 1:2004516/35‑1 (MQ=255) gggAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGtt > 1:3634674/1‑71 (MQ=255) gggAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGt > 1:2467896/1‑70 (MQ=255) gAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGAc > 1:413722/1‑45 (MQ=255) gAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACa > 1:2123634/1‑46 (MQ=255) gAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGt > 1:2456960/1‑71 (MQ=255) aaGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTc < 1:2319998/71‑1 (MQ=255) aaGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTc > 1:1336518/1‑71 (MQ=255) aaGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTc < 1:1906890/71‑1 (MQ=255) gTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACgg < 1:160739/55‑1 (MQ=255) gTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGa < 1:3544102/71‑1 (MQ=255) tGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGa > 1:4475492/1‑40 (MQ=255) tGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCg < 1:1950382/62‑1 (MQ=255) gACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATg > 1:3879578/1‑71 (MQ=255) accaccGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATg < 1:3352103/70‑1 (MQ=255) accaccGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGt > 1:2336300/1‑71 (MQ=255) ccaccGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGa > 1:1976330/1‑67 (MQ=255) ccaccGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGtt < 1:4639163/71‑1 (MQ=255) ccaccGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGtt < 1:3574525/71‑1 (MQ=255) caccGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCttt < 1:3104329/46‑1 (MQ=255) caccGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACg < 1:1339423/49‑1 (MQ=255) caccGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTg < 1:2919420/71‑1 (MQ=255) accGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGa < 1:1148689/71‑1 (MQ=255) accGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGa < 1:627434/71‑1 (MQ=255) accGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGa < 1:3168383/71‑1 (MQ=255) accGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGa < 1:3530727/71‑1 (MQ=255) accGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGa < 1:3476653/71‑1 (MQ=255) cGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCtt < 1:3187239/42‑1 (MQ=255) ttAGCCTCAACATGGCGGCGAATGGCATCGACAGAAc > 1:404082/1‑37 (MQ=255) ttAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATg < 1:3310252/51‑1 (MQ=255) ttAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGtt > 1:845094/1‑65 (MQ=255) aGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGa > 1:2441072/1‑59 (MQ=255) cTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATc > 1:239139/1‑64 (MQ=255) cTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCACGCTCa > 1:3013104/1‑71 (MQ=255) tCAACATGGCGACGAATGGCATCGACAGAACCTTTa > 1:293129/1‑36 (MQ=255) tCAACATGGCGACGAATGGCATCGACAGAACCTTTa > 1:4292461/1‑36 (MQ=255) tCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCg < 1:4462608/54‑1 (MQ=255) tCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGa < 1:3215322/61‑1 (MQ=255) tCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGa < 1:2508572/61‑1 (MQ=255) cAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATc > 1:2798908/1‑41 (MQ=255) cAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATc > 1:841839/1‑71 (MQ=255) aaCATGGCGACGAATGGCATCGACAGAACCTTTACGGa > 1:1298096/1‑38 (MQ=255) aaCATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGAt > 1:948725/1‑54 (MQ=255) aaCATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGAt > 1:3436475/1‑54 (MQ=255) aaCATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCACGCTCATcc > 1:2849940/1‑71 (MQ=255) cATGGCGACGAATGGCATCGACAGAACCTTTACgg < 1:3015406/35‑1 (MQ=255) aTGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCgg < 1:1679382/43‑1 (MQ=255) tGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCt < 1:2644566/71‑1 (MQ=255) tGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCt < 1:4042206/71‑1 (MQ=255) cgacgaATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTg < 1:2886631/71‑1 (MQ=255) acgaATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTgcg < 1:716862/71‑1 (MQ=255) | CGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGCG > W3110S.gb/726185‑726323 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |