| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 732,330 | A→G | 6.5% | N776D (AAC→GAC) | rhsC → | rhsC element core protein RshC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 732,330 | 0 | A | G | 6.5% | 164.4 / 2.7 | 61 | N776D (AAC→GAC) | rhsC | rhsC element core protein RshC |
| Reads supporting (aligned to +/- strand): ref base A (34/23); new base G (2/2); total (36/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACC > W3110S.gb/732262‑732392 | aaCACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAAc > 1:2972887/1‑71 (MQ=255) tGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAg > 1:2341462/1‑71 (MQ=255) gACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAgg > 1:1058157/1‑71 (MQ=255) aCGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAgg < 1:1106223/70‑1 (MQ=255) tGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTg > 1:4276666/1‑71 (MQ=255) gCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTgg > 1:975059/1‑71 (MQ=255) gCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTgg > 1:4092838/1‑71 (MQ=255) gCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTgg > 1:1484243/1‑71 (MQ=255) gCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTg > 1:3261715/1‑70 (MQ=255) tcatcCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAgg < 1:667809/62‑1 (MQ=255) atcCGCAGACGAATGAACTGCTCTGGCAGCATGAGCCCAGACATGCGTACAACGCACAGGGACTGGCGAAc > 1:874186/1‑71 (MQ=255) atcCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACaa > 1:2472307/1‑52 (MQ=255) atcCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACGACGCACAGGGACTGGCGAAc > 1:2490315/1‑71 (MQ=255) atcCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGcac > 1:2994191/1‑57 (MQ=255) atcCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGa > 1:3504638/1‑62 (MQ=255) atcCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGaa > 1:523685/1‑70 (MQ=255) atcCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAAc > 1:4557494/1‑71 (MQ=255) tcCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGAc > 1:3305658/1‑62 (MQ=255) tcCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAAcc > 1:405540/1‑71 (MQ=255) tcCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAAcc > 1:2789208/1‑71 (MQ=255) tcCGCAGACGAATGAACTGCTCTGGCAGCATGAGACAAGACATGCGTACAACGCACAggg > 1:308689/1‑60 (MQ=255) cGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACGACGCACAg > 1:2795117/1‑56 (MQ=255) cGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCg < 1:3762584/70‑1 (MQ=255) cGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGc > 1:393761/1‑71 (MQ=255) cGCAGACGAATGAACTGCTCTGGCAGCATGAGACAAGACATGCGTACAACGCACAGGGACTGGCGAACCGc > 1:1202682/1‑71 (MQ=255) gCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCt < 1:3758036/71‑1 (MQ=255) cAGACGAATGAACTGTTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTg > 1:3322683/1‑71 (MQ=255) cAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTg < 1:2645399/71‑1 (MQ=255) cAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTg > 1:3201195/1‑71 (MQ=255) gACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGca > 1:2319324/1‑49 (MQ=255) aCGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAggg < 1:2487642/53‑1 (MQ=255) aCGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGta < 1:655697/70‑1 (MQ=255) gAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGc < 1:1179663/57‑1 (MQ=255) aTGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACGACGca < 1:3351416/44‑1 (MQ=255) aTGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACGACGca < 1:690712/44‑1 (MQ=255) tGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCg > 1:3240401/1‑70 (MQ=255) aaCTGCTCTGGCAGCATGAGACCAGACATGCGTACaa > 1:605168/1‑37 (MQ=33) aaCTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCg < 1:3969282/68‑1 (MQ=255) cTGCTCTGGCAGCATGAGACCAGTCATGCGTACAACGCACAGGGACTGGCg < 1:3694050/51‑1 (MQ=255) cTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAg > 1:3851349/1‑71 (MQ=39) cTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAg < 1:219342/71‑1 (MQ=39) tGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTg < 1:2143188/58‑1 (MQ=255) tGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAg < 1:1202582/70‑1 (MQ=40) ctGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGc > 1:4071680/1‑71 (MQ=39) gcagcaTGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCccg > 1:3748315/1‑71 (MQ=39) agcaTGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCccgcc < 1:3283210/71‑1 (MQ=39) tGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTTCCCGCCGTgg < 1:4567053/71‑1 (MQ=255) tGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTgg < 1:1461225/71‑1 (MQ=39) tGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTgg < 1:4583843/71‑1 (MQ=39) agaCCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATAc > 1:2946168/1‑48 (MQ=255) agaCCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCTTGGaa < 1:3615396/71‑1 (MQ=255) gaCCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACa < 1:2430967/53‑1 (MQ=255) gaCCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAAt > 1:275924/1‑71 (MQ=39) aCCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTg < 1:1269862/41‑1 (MQ=255) cAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGc > 1:2048696/1‑71 (MQ=39) aGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCt < 1:3412096/71‑1 (MQ=39) gACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTg < 1:4371816/71‑1 (MQ=39) cATGCGTACAACGCACAGGGACTGGCGAACCGCTGtata > 1:1271688/1‑39 (MQ=255) cATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTg < 1:1837190/51‑1 (MQ=255) aTGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGAcc > 1:3241984/1‑71 (MQ=39) tGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGa > 1:583308/1‑68 (MQ=40) aCAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGa > 1:1922711/1‑63 (MQ=40) cAACGCACAGGGACTGGCGAACCGCTGTATACCGGa > 1:120625/1‑36 (MQ=255) | AACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACC > W3110S.gb/732262‑732392 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |