| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 771,768 | C→T | 15.5% | intergenic (+735/‑112) | mngB → / → cydA | alpha‑mannosidase/cytochrome d terminal oxidase, subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 771,768 | 0 | C | T | 15.5% | 147.3 / 17.4 | 58 | intergenic (+735/‑112) | mngB/cydA | alpha‑mannosidase/cytochrome d terminal oxidase, subunit I |
| Reads supporting (aligned to +/- strand): ref base C (24/25); new base T (4/5); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01 | |||||||||||
TATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAAC > W3110S.gb/771699‑771835 | tatataCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCa < 1:4169359/71‑1 (MQ=255) tataCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACATTTCTTTACGCCGTTTTTGTGTGCAt > 1:2996796/1‑70 (MQ=255) ataCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCAtt > 1:2540883/1‑70 (MQ=255) taCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTc < 1:587331/70‑1 (MQ=255) taCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTc < 1:1532349/70‑1 (MQ=255) aCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCa > 1:460000/1‑66 (MQ=255) aCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTca > 1:305889/1‑70 (MQ=255) ggATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTcaca < 1:920116/67‑1 (MQ=255) gATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCATTACGCCGTTTTTGTGTGCATTAACAt < 1:2254795/67‑1 (MQ=255) cAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCa > 1:3957877/1‑57 (MQ=255) cAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTatga > 1:41017/1‑71 (MQ=255) aTTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGTATTCACATTGTatgatg < 1:3703340/71‑1 (MQ=255) tttGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCAGTTTTTGTGTGCATTCACATGGTatgatg < 1:4516287/70‑1 (MQ=255) tGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTatgatg > 1:1469959/1‑68 (MQ=255) tttttATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCa < 1:762251/48‑1 (MQ=255) tttATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCAt > 1:2878452/1‑47 (MQ=255) tttATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTcaca > 1:2692625/1‑52 (MQ=255) tttATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTatgat > 1:1647461/1‑61 (MQ=255) tttATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTatga > 1:2896155/1‑60 (MQ=255) tttATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGtt > 1:1860158/1‑70 (MQ=255) tttATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGtt > 1:1291201/1‑70 (MQ=255) tattaACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGTATTCACATGGTATGATGAAAGTGTTca > 1:2385800/1‑70 (MQ=255) ttaACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGTATTCACATGGTATGATGAAAGTGTTcaaac < 1:3138458/71‑1 (MQ=255) aCATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTAtgatga < 1:4356339/56‑1 (MQ=255) cATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAAt < 1:882404/71‑1 (MQ=255) cATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTCCATTCACATGGTATGATGAAAg > 1:2017331/1‑58 (MQ=255) aTGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTatgatg < 1:2438291/53‑1 (MQ=255) tGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGTATTCACATGGTATGATGaa < 1:3965964/54‑1 (MQ=255) tGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCa < 1:76991/36‑1 (MQ=255) tGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGaaa > 1:2805230/1‑55 (MQ=255) tttGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTc < 1:114953/70‑1 (MQ=255) tttGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCt < 1:2048198/71‑1 (MQ=255) gCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTAtt > 1:3050757/1‑71 (MQ=255) gCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTAtt > 1:3045176/1‑71 (MQ=255) gCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTAt < 1:2639940/70‑1 (MQ=255) ccTTTCTTTACGCCGTTTTTGTGTGTATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTgggg < 1:1442619/71‑1 (MQ=255) ctttctttACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAg > 1:2286696/1‑46 (MQ=255) ctttctttACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTcaaacaa < 1:344425/57‑1 (MQ=255) tttctttACGCCGTTTTTGTGTGTATTCACATGGTatga > 1:731875/1‑39 (MQ=255) tttctttACGCCGTTTTTGTGTGTATTCACATGGTatga > 1:679196/1‑39 (MQ=255) tttctttACGCCGTTTTTGTGTGTATTCACATGGTatga > 1:401647/1‑39 (MQ=255) tttctttACGCCGTTTTTGTGTGTATTCACATGGTatg < 1:1209678/38‑1 (MQ=255) tttctttACGCCGTTTTTGTGTGCATTCACATGGTAtgatga < 1:2252208/42‑1 (MQ=255) ttctttACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAAt > 1:1176435/1‑57 (MQ=255) tctttACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTAtt > 1:1812408/1‑63 (MQ=255) ctttACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTgg > 1:2743081/1‑64 (MQ=255) tttACGACGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTAtt < 1:544219/61‑1 (MQ=255) cGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCgtgt < 1:2628694/70‑1 (MQ=255) cGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCgtgt < 1:3532469/70‑1 (MQ=255) cGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGtgtg < 1:1240377/71‑1 (MQ=255) tttGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTAtt > 1:4449847/1‑50 (MQ=255) tttGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTgggg > 1:1864102/1‑54 (MQ=255) tttGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCgt > 1:610138/1‑61 (MQ=255) gtgtgCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCt < 1:3141140/70‑1 (MQ=255) tgtgCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTa < 1:4314068/70‑1 (MQ=255) tgCATTCACATGGTATGATTAAAGTGTTCAAACAAATTTCTATTGGGGCATGCgtgt < 1:1812208/57‑1 (MQ=255) tgCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCt < 1:1638397/67‑1 (MQ=255) tgCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAAc < 1:424325/70‑1 (MQ=255) | TATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTAAC > W3110S.gb/771699‑771835 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |