| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 772,842 | G→A | 13.9% | G321G (GGG→GGA) | cydA → | cytochrome d terminal oxidase, subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 772,842 | 0 | G | A | 13.9% | 195.4 / 19.5 | 72 | G321G (GGG→GGA) | cydA | cytochrome d terminal oxidase, subunit I |
| Reads supporting (aligned to +/- strand): ref base G (33/29); new base A (6/4); total (39/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
CCGTGGATACCCCGGTTATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAGGCGGTTCGTGACCAGTTC > W3110S.gb/772775‑772911 | ccGTGGATACCCCGGTTATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGAt > 1:4506432/1‑70 (MQ=255) cGTGGATACCCCGGTTATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATg < 1:3231393/70‑1 (MQ=255) cGTGGATACCCCGGTTATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATg < 1:486327/70‑1 (MQ=255) gTGGATACCCCGGTTATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGaa < 1:3921716/71‑1 (MQ=255) aTACCCCGGTTATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCg < 1:1829540/71‑1 (MQ=255) aCCCCGGTTATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTa > 1:2590190/1‑71 (MQ=255) ccGGTTATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTActc > 1:1036348/1‑71 (MQ=255) cGGTTATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTActct > 1:1500584/1‑71 (MQ=255) ggTTATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTActctc > 1:4452400/1‑71 (MQ=255) ggTTATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTActctc > 1:3844527/1‑71 (MQ=255) gTTATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTActctct > 1:1731364/1‑71 (MQ=255) gTTATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTActctct > 1:639878/1‑71 (MQ=255) gTTATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTActctc > 1:2837938/1‑70 (MQ=255) tATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAGCGCATTCGTAACGGGATGAAGGCGTACTCTCTGc > 1:1850529/1‑71 (MQ=255) tATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTACTCTCTGc > 1:816677/1‑71 (MQ=255) aTCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGaa > 1:4121288/1‑56 (MQ=255) tCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTa > 1:3972731/1‑61 (MQ=255) ggCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCg < 1:3193464/70‑1 (MQ=255) tGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTa > 1:2293831/1‑55 (MQ=255) aaaGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTATTCTCTGCTCGAACAACt < 1:1405965/71‑1 (MQ=255) aaaGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGaacaa < 1:3018290/69‑1 (MQ=255) aaGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTa < 1:2179208/52‑1 (MQ=255) aaGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTa < 1:1614872/52‑1 (MQ=255) aaGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTg > 1:4375141/1‑71 (MQ=255) aaGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGAATGAAGGCGTACTCTCTGCTCGAACAACTg < 1:2258906/71‑1 (MQ=255) aaGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGAATGAAGGCGTACTCTCTGCTCGAACAACTg < 1:2805585/71‑1 (MQ=255) gagCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCg > 1:479594/1‑71 (MQ=255) agCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGt > 1:2127138/1‑71 (MQ=255) cTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATg < 1:2608098/39‑1 (MQ=255) cTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTc < 1:3494819/71‑1 (MQ=255) tGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTc < 1:1000521/70‑1 (MQ=255) gATGGTGCAGCATGAAGAACGCATTCGTAACGGGATg < 1:3184336/37‑1 (MQ=255) gATGGTGCAGCATGAAGAACGCATTCGTAACGGGATg < 1:2746842/37‑1 (MQ=255) tGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGt > 1:40583/1‑71 (MQ=255) agcaTGAAGAACGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAAGAACTGCGTTCTGGTTCTAcc < 1:1786297/71‑1 (MQ=255) agcaTGAAGAACGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTAcc < 1:2152436/71‑1 (MQ=255) gcaTGAAGAACGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCg < 1:2299476/71‑1 (MQ=255) caTGAAGAACGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTc > 1:3304847/1‑59 (MQ=255) caTGAAGAACGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGa > 1:1606913/1‑71 (MQ=255) aTGAAGAACGCATTCGTAACGGAATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGAc > 1:1725201/1‑71 (MQ=255) aTGAAGAACGCATTCGTAACGGAATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGAc > 1:3659125/1‑71 (MQ=255) tGAAGAACGCATTCGTAACGGAATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGAc > 1:3638903/1‑70 (MQ=255) gaagaaCGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGa > 1:4130551/1‑68 (MQ=255) aagaaCGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAg > 1:4461582/1‑71 (MQ=255) aagaaCGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAg > 1:3529272/1‑71 (MQ=255) gaaCGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTAcc < 1:3835193/63‑1 (MQ=255) gaaCGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGAc > 1:2342336/1‑66 (MQ=255) gaaCGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAga > 1:2054973/1‑69 (MQ=255) gaaCGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAGGc < 1:2649124/71‑1 (MQ=255) aaCGCATTCGTAACTGGATGAAGGCGTACTCTCTGCTCGaaca < 1:789045/43‑1 (MQ=255) aCGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGa > 1:4401553/1‑63 (MQ=255) cATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCg < 1:3177711/59‑1 (MQ=255) aTTCGTAACGGAATGAAGGCGTACTCTCTGCTCGa < 1:553619/35‑1 (MQ=255) ttCGTAACGGGATGAAGGCGTACTCTCTGCTCGaac < 1:4515159/36‑1 (MQ=255) ttCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCa < 1:981492/61‑1 (MQ=255) ttCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCa < 1:2409122/61‑1 (MQ=255) ttCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAGGCGGTTc < 1:2717951/69‑1 (MQ=255) ttCGTAACGGAATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAGGCGGTTCGt < 1:2550478/71‑1 (MQ=255) tCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGt > 1:626760/1‑49 (MQ=255) cGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCa > 1:335174/1‑59 (MQ=255) cGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAGGCGGTTCGTGa > 1:4392651/1‑71 (MQ=255) cGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACAAGGCGGTTCGTGa > 1:1630366/1‑71 (MQ=255) cGTAACGGAATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAGGCGGTTCGTGa > 1:966120/1‑71 (MQ=255) cGTAACGGAATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAGGCGGTTCGTGa > 1:3065650/1‑71 (MQ=255) cGTAACGGAATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAGGCGGTTCGTGa > 1:659234/1‑71 (MQ=255) tAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAGGCGGTTCGTGAcc < 1:3251044/71‑1 (MQ=255) aCGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTa > 1:1721756/1‑48 (MQ=255) aCGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTAc > 1:742801/1‑49 (MQ=255) aCGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAGGCGGTTCGTGACCAg < 1:2111975/71‑1 (MQ=255) aCGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAGGCGGTTCGTGACCAg > 1:1858144/1‑71 (MQ=255) aCGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAGGCGGTTCGTGACCAg < 1:2925619/71‑1 (MQ=255) cGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGAc < 1:42494/52‑1 (MQ=255) ggATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAGGCGGTTCGTGACCAGTTc < 1:474843/71‑1 (MQ=255) ggATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAGGCGGTTCGTGACCAGTTc < 1:3881896/71‑1 (MQ=255) | CCGTGGATACCCCGGTTATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAACGGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAGGCGGTTCGTGACCAGTTC > W3110S.gb/772775‑772911 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |