| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 823,380 | G→A | 21.6% | P261L (CCG→CTG) | ybhO ← | cardiolipin synthase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 823,380 | 0 | G | A | 21.6% | 74.5 / 16.1 | 37 | P261L (CCG→CTG) | ybhO | cardiolipin synthase 2 |
| Reads supporting (aligned to +/- strand): ref base G (14/15); new base A (2/6); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01 | |||||||||||
CAAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGT > W3110S.gb/823315‑823449 | cAAAAACCTGAACGCCGGCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCata > 1:2410885/1‑71 (MQ=255) aaaaCCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCAGCata < 1:1169642/69‑1 (MQ=255) aaCCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCg > 1:2684488/1‑71 (MQ=255) aaCCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCAGCATATCCg > 1:824089/1‑71 (MQ=255) aCCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCAGCATATCCgg < 1:2663142/71‑1 (MQ=255) aaCGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGc > 1:4072797/1‑71 (MQ=255) ccgccTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATc > 1:1439644/1‑59 (MQ=255) ccgccTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTg < 1:1564394/71‑1 (MQ=255) tAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGc > 1:4196729/1‑47 (MQ=255) tAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGc > 1:4461437/1‑60 (MQ=255) tAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGAt > 1:2025142/1‑70 (MQ=255) tAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGAt > 1:1161875/1‑70 (MQ=255) tAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCACTGAATGAt > 1:3210313/1‑70 (MQ=255) aaCCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGa < 1:1401327/64‑1 (MQ=255) aGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGttt < 1:1232045/71‑1 (MQ=255) gATAGTTATACAGCAAGCGCGCACCGACTCTGGCAATCAGCATATCCGGTTCGCCCTGAATGATCAGTTTg < 1:2293729/71‑1 (MQ=255) gATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCAGCATATCCGGTTCGCCCTGAATGATCAGTTTg < 1:1426479/71‑1 (MQ=255) gTTATACAGCAAGCGCGCACCGACTCTGACAATCAGCATATCCGGTTCGCCCTGAATGATCAGTTTGATc < 1:1521729/70‑1 (MQ=255) aGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGtt > 1:3607570/1‑40 (MQ=255) cAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCa > 1:1034457/1‑53 (MQ=255) cAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCcgc > 1:2573184/1‑71 (MQ=255) gcACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGc < 1:3039675/71‑1 (MQ=255) cGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATg < 1:699104/38‑1 (MQ=255) cGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGa < 1:3088176/39‑1 (MQ=255) cGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGa < 1:4442449/39‑1 (MQ=255) cGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGt < 1:3892627/44‑1 (MQ=255) cGACTCTGACAATCAGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCt > 1:2707527/1‑71 (MQ=255) gACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGAt < 1:63537/39‑1 (MQ=255) gACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCtt < 1:2498898/71‑1 (MQ=255) aCTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCtt > 1:259420/1‑70 (MQ=255) ctctGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCa < 1:1127162/51‑1 (MQ=255) tctGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTg < 1:3272185/43‑1 (MQ=255) tctGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTg < 1:50711/43‑1 (MQ=255) aCAATCGGCATATCCGGTTCCCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGc > 1:1182055/1‑69 (MQ=255) aCAATCAGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCa < 1:4023156/70‑1 (MQ=255) aTCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGa < 1:4065405/37‑1 (MQ=255) aTCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCAcc < 1:1535656/45‑1 (MQ=255) ggCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCgt < 1:34168/70‑1 (MQ=255) | CAAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGT > W3110S.gb/823315‑823449 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |