| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 865,658 | A→G | 19.6% | F377L (TTT→CTT) | moeA ← | molybdopterin biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 865,658 | 0 | A | G | 19.6% | 115.3 / 24.9 | 56 | F377L (TTT→CTT) | moeA | molybdopterin biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base A (26/19); new base G (8/3); total (34/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01 | |||||||||||
CCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGGCGTTGCGTTGC > W3110S.gb/865589‑865728 | ccACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAg > 1:2018503/1‑71 (MQ=255) ccATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGc > 1:4155160/1‑71 (MQ=255) cATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGc > 1:4600159/1‑70 (MQ=255) cATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCt < 1:4613430/71‑1 (MQ=255) aTTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCt > 1:272148/1‑70 (MQ=255) tCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTaaa > 1:4391956/1‑62 (MQ=255) tCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAg > 1:674598/1‑63 (MQ=255) tCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAg > 1:1791819/1‑63 (MQ=255) gCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAata > 1:2977414/1‑71 (MQ=255) gCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAat < 1:1281006/70‑1 (MQ=255) cccACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAGGGAGCTAAatat < 1:3136602/71‑1 (MQ=255) cACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAatata < 1:4544011/70‑1 (MQ=255) cTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAGGGAGCTAAATATAtgt > 1:2042925/1‑71 (MQ=255) ttCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATAtgt > 1:3780688/1‑70 (MQ=255) tCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTaaa > 1:715682/1‑53 (MQ=255) tCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAGGGAGCTAAATATAtg > 1:370164/1‑68 (MQ=255) tCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAGGGAGCTAAATATAtg > 1:3292751/1‑68 (MQ=255) tCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAGGGAGCTAAATATAtg > 1:192455/1‑68 (MQ=255) tCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAatat > 1:3246257/1‑65 (MQ=255) tCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATAtgtg > 1:3240636/1‑70 (MQ=255) ccACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATAtgtg < 1:141158/69‑1 (MQ=255) ccACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGaa > 1:2943096/1‑71 (MQ=255) cacaTTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGaa < 1:1297672/70‑1 (MQ=255) cacaTTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGaa < 1:1552422/70‑1 (MQ=255) cacaTTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGaa < 1:1557932/70‑1 (MQ=255) ttGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCGGAGGCTAAAGGAGCTAAATATAtg > 1:2142678/1‑62 (MQ=255) ttGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAGGGa > 1:3420735/1‑50 (MQ=255) ttGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAGGGAGCTAAatat < 1:765073/59‑1 (MQ=255) ttGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAGGGAGCTAAATATATGTGAAccc > 1:3415221/1‑69 (MQ=255) ttGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAg < 1:3025270/48‑1 (MQ=255) gCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGa > 1:94087/1‑70 (MQ=255) ccGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGaa < 1:4002946/63‑1 (MQ=255) ccGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGAt < 1:3750946/70‑1 (MQ=255) cgATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTcc > 1:4088995/1‑71 (MQ=255) cgATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTcc > 1:3792286/1‑71 (MQ=255) gATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAGGGAGCTAAATATATGTGAACCCTGATGTCCg < 1:1635535/71‑1 (MQ=255) aTCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTaaa < 1:4224707/48‑1 (MQ=255) aTCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCgg < 1:3530103/71‑1 (MQ=255) cgcgTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAg > 1:3984709/1‑38 (MQ=255) cgcgTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAg > 1:3110565/1‑38 (MQ=255) cgcgTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGa > 1:3121069/1‑62 (MQ=255) cgcgTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCggtg > 1:1431679/1‑71 (MQ=255) gcgTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAatata > 1:3060611/1‑49 (MQ=255) tCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATAtg > 1:3538509/1‑47 (MQ=255) ccAGCACGATAAAGCAGTTGCCGAGGCTAAGGGAGCTAAATATATGTGAACCCTGATGTCCGGTGgtcgt > 1:3910164/1‑70 (MQ=255) ccAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGgtcgt > 1:3241397/1‑70 (MQ=255) ccAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGgtcgt < 1:3634156/70‑1 (MQ=255) cAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAata > 1:309289/1‑41 (MQ=255) tAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGt > 1:192897/1‑71 (MQ=255) aaaGCAGTTGCCGAGGCTAAGGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGtt > 1:2346827/1‑71 (MQ=255) cAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGcc < 1:484708/71‑1 (MQ=255) cAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGcc < 1:2978195/71‑1 (MQ=255) gTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTg < 1:3573892/39‑1 (MQ=255) tGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATc > 1:1573005/1‑70 (MQ=255) ccGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCgg < 1:150344/70‑1 (MQ=255) ccGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGgc < 1:1198608/71‑1 (MQ=255) aGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGgcgtt > 1:196502/1‑71 (MQ=255) cTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGgc < 1:1499776/65‑1 (MQ=255) aaGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGGcgttgcgttg < 1:2004905/71‑1 (MQ=255) aGGAGCTAAATATATGTGAACCCTGATGTCCGGTGgtcgtc < 1:1291795/41‑1 (MQ=255) aGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGGCgttgcgttgc < 1:3910650/71‑1 (MQ=255) aGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGGCgttgcgttgc < 1:3300355/71‑1 (MQ=255) | CCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGGCGTTGCGTTGC > W3110S.gb/865589‑865728 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |