| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 980,276 | C→T | 15.4% | P1177S (CCG→TCG) | mukB → | fused chromosome partitioning proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 980,276 | 0 | C | T | 15.4% | 97.6 / 10.2 | 39 | P1177S (CCG→TCG) | mukB | fused chromosome partitioning proteins |
| Reads supporting (aligned to +/- strand): ref base C (15/18); new base T (4/2); total (19/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GGTGCGCTGCGTCTGGCGGTGGCGGATAACGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTGGCGGTTTATCAGCATCTGCGTGAACGTATTCGT > W3110S.gb/980210‑980344 | ggTGCGCTGCGTCTGGCGGTGGCGGATAACGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATTCGa < 1:1426952/70‑1 (MQ=255) gTGCGCTGCGTCTGGCGGTGGCGGATAACGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGaa < 1:4152585/70‑1 (MQ=255) gTGCGCTGCGTCTGGCGGTGGCGGATAACGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGaa < 1:2399903/70‑1 (MQ=255) cTGCGTCTGGCGGTGGCGGATAACGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGAAACGTcc > 1:3202939/1‑71 (MQ=255) cGTCTGGCGGTGGCGGATAACGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGAAACGTCCg < 1:4081690/69‑1 (MQ=255) gcggtggcggATAACGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGAAACGTCCGGAGCGTaa < 1:1711764/71‑1 (MQ=255) gcggtggcggATAACGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGAAACGTCCGGAGCGTa > 1:225325/1‑70 (MQ=255) gtggcggATAGCGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGAAACGTCCGGAGCGTAAAAt < 1:2953147/71‑1 (MQ=255) tggcggATAACGAACATCTGCGCGACGTGCTCCGCATGTCGGAAGATCCGAAACGTCCGGAGc < 1:2945614/63‑1 (MQ=255) gcggATAACGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGAAACGTCCGGAGCGTAAAATTc < 1:2711068/70‑1 (MQ=255) aTAACGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGaaa > 1:498637/1‑47 (MQ=255) tAACGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGAAACGTCCGGAGc < 1:1597985/56‑1 (MQ=255) aaCGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGAAACGTCCGGAGCGTAAAATTCa > 1:858597/1‑65 (MQ=255) aCGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGAAACGTCCGGAGCGTAAAATTCAGttcttc < 1:3353632/71‑1 (MQ=255) aCGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGAAACGTCCGGAGCGTAAAATTCAGttcttc < 1:289106/71‑1 (MQ=255) cGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATTCGaaa > 1:2157926/1‑43 (MQ=255) cGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATTCGaaa > 1:3101980/1‑43 (MQ=255) cGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATTCGaaa > 1:1781680/1‑43 (MQ=255) aCATCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTgg > 1:2143513/1‑71 (MQ=255) tCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGAAACGTCCGGAGCGTaaaa > 1:589310/1‑53 (MQ=255) tCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGAAACGTCCGGAGCGTAAAATTCAGttctt < 1:3316740/63‑1 (MQ=255) tGCGCGACGTGCTGCGCATGTCGGAAGATCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCg > 1:3464303/1‑63 (MQ=255) cgcgACGTGCTGCGCATGTCGGAAGATCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTGGCGGt < 1:1309516/68‑1 (MQ=255) cGTGCTGCGCATGTCGGAAGATCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTGGCGGTTTATCAGc < 1:1818885/71‑1 (MQ=255) cgcATGTCGGAAGATCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTGGCGGTTTATCAGCATCTGc < 1:1359242/70‑1 (MQ=255) gcATGTCGGAAGATCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTGGc < 1:2231087/52‑1 (MQ=255) aTGTCGGAAGATCCGAAACGTCCGGAGCGTAAAATTCAGttctt > 1:2989629/1‑44 (MQ=255) aTGTCGGAAGATCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTGGCgg > 1:3153564/1‑52 (MQ=255) tGTCGGAAGATTCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTGGCGGTTTAt < 1:899854/56‑1 (MQ=255) tCGGAAGATTGGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTg > 1:2671617/1‑45 (MQ=38) tCGGAAGATCCGAAACGTCCGGAGCGTAAAATTCAGtt > 1:296283/1‑38 (MQ=255) tCGGAAGATCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTGGCGGTTTATCa > 1:1350774/1‑56 (MQ=255) ggAAGATCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTGGCGGTTTATCAGCATCTGCGTGaa > 1:1264224/1‑67 (MQ=255) ggAAGATCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTGGCGGTTTATCAGCATCTGCGTGAACGTa < 1:2533246/71‑1 (MQ=255) ggAAGATCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTGGAGGTTTATCAGCATCTGCGTGAACGt > 1:1888710/1‑70 (MQ=255) gATCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTGGCGGTTTATCAGCATCTGCGTGAACGTATTCg < 1:3813622/71‑1 (MQ=255) aTCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTGGCGGTTTATCAGCATCTGCGTGAACGTATTCGt < 1:2661716/71‑1 (MQ=255) tCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTGGCGGTTTATCAGCATCTGCGTGAACGTATTCGt > 1:2731105/1‑70 (MQ=255) tCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTGGCGGTTTATCAGCATCTGCGTGAACGTATTCGt > 1:1363743/1‑70 (MQ=255) | GGTGCGCTGCGTCTGGCGGTGGCGGATAACGAACATCTGCGCGACGTGCTGCGCATGTCGGAAGATCCGAAACGTCCGGAGCGTAAAATTCAGTTCTTCGTGGCGGTTTATCAGCATCTGCGTGAACGTATTCGT > W3110S.gb/980210‑980344 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |