| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,023,649 | G→A | 16.0% | L219L (CTC→CTT) | yccS ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,023,649 | 0 | G | A | 16.0% | 125.3 / 15.0 | 50 | L219L (CTC→CTT) | yccS | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (18/24); new base A (5/3); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
AGTAATAATGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAATCGTACAGCGGT > W3110S.gb/1023582‑1023712 | aGTAATAATGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGt > 1:673848/1‑71 (MQ=255) ataatGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTc < 1:2892790/70‑1 (MQ=255) ataatGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTc < 1:4596887/70‑1 (MQ=255) ataatGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCg < 1:3678278/71‑1 (MQ=255) ataatGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCg > 1:2316233/1‑71 (MQ=255) ataatGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGAGGGTCAGCAGCGAGAGTTTc < 1:214137/70‑1 (MQ=255) taatGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGttt < 1:4440403/68‑1 (MQ=255) taatGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGttt < 1:2074126/68‑1 (MQ=255) aatGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTc > 1:1781356/1‑71 (MQ=255) gCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAAAGTTTCGTCTGa > 1:1776300/1‑71 (MQ=255) cAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGGAGCGAAAGTTTCGTCTGAt > 1:3203838/1‑71 (MQ=255) cAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGa < 1:1814660/70‑1 (MQ=255) cAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGAt < 1:4109800/71‑1 (MQ=255) cAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGAt > 1:4178581/1‑71 (MQ=255) gcgTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCg > 1:1626087/1‑54 (MQ=255) ttCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTc < 1:116138/50‑1 (MQ=255) tCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAAAGTTTCGTCTGATTCAATGTCGCCAt > 1:4504540/1‑69 (MQ=255) tCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAAAGTTTCGTCTGATTCAATGTCGCCAt > 1:555837/1‑69 (MQ=255) ccccGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCg > 1:2622275/1‑49 (MQ=255) cGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTTAATGTAGCCATcag > 1:3911930/1‑68 (MQ=255) cGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATcag > 1:3682699/1‑68 (MQ=255) ttGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTc > 1:4551416/1‑43 (MQ=255) ttGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGt > 1:2055357/1‑45 (MQ=255) aCCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGt < 1:161767/71‑1 (MQ=255) ccACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGtt < 1:3807926/71‑1 (MQ=255) ccACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGt < 1:9777/70‑1 (MQ=255) cACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTc < 1:2913916/47‑1 (MQ=255) aTCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGc > 1:1879979/1‑69 (MQ=255) aTCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCga < 1:750954/71‑1 (MQ=255) tCGCCACGTAAGCTGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCgag < 1:4381982/71‑1 (MQ=255) gCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCg < 1:4441897/67‑1 (MQ=255) aCGTAAGCGGGTCAGCAGCGAGAGTTTCGGCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCa < 1:1788600/71‑1 (MQ=255) cGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATc < 1:3370915/48‑1 (MQ=255) gTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCaa < 1:2136613/70‑1 (MQ=255) gTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCaa < 1:418825/70‑1 (MQ=255) gTAAGCGGGTCAGCAGCGAAAGTTTCGTCTGATTCAAt < 1:2512384/38‑1 (MQ=255) tAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCaa > 1:2269900/1‑36 (MQ=255) tAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAAt > 1:67223/1‑71 (MQ=255) aaGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCaa > 1:4351709/1‑35 (MQ=255) aGCGGGTCAGCAGCGAAAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAATCg < 1:4020053/71‑1 (MQ=255) gCGGGTCAGCAGCGAAAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAATCGt < 1:1029643/71‑1 (MQ=255) gggTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGcc > 1:818480/1‑38 (MQ=255) gggTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCa > 1:1814980/1‑39 (MQ=255) tCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATcag < 1:2722667/40‑1 (MQ=255) tCAGCAGCGAAAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAATCGTACAGc > 1:1481451/1‑71 (MQ=255) cagcagCGAGAGTTTCGTCTGATTCAATGTCGCCATcagca > 1:210040/1‑41 (MQ=255) agcagcGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAATCGTACAGCgg < 1:1397818/71‑1 (MQ=255) gcagcGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAATCGTACAGCGGt < 1:1730276/71‑1 (MQ=255) gcaccGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAATCGTACAGCGGt < 1:2776377/71‑1 (MQ=255) cagcGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAATCGTACAGCGGt > 1:3800214/1‑70 (MQ=255) | AGTAATAATGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAATCGTACAGCGGT > W3110S.gb/1023582‑1023712 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |