| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,044,904 | T→C | 20.4% | E65E (GAA→GAG) | etp ← | phosphotyrosine‑protein phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,044,904 | 0 | T | C | 20.4% | 111.3 / 25.3 | 54 | E65E (GAA→GAG) | etp | phosphotyrosine‑protein phosphatase |
| Reads supporting (aligned to +/- strand): ref base T (25/18); new base C (5/6); total (30/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01 | |||||||||||
ACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGAC > W3110S.gb/1044837‑1044971 | aCTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCCTCa < 1:2127862/71‑1 (MQ=255) tCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTc > 1:3095725/1‑68 (MQ=255) cATTGCCAGAATCAGATCGTAATTTCGTTCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAaga < 1:3126022/70‑1 (MQ=255) cATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCaa > 1:1668616/1‑68 (MQ=255) cATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCaa > 1:897910/1‑68 (MQ=255) cATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAaga < 1:2878714/70‑1 (MQ=255) cATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAaga < 1:4042449/70‑1 (MQ=255) cATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAaga < 1:313781/70‑1 (MQ=255) cATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCCTCaa > 1:2753231/1‑68 (MQ=255) cATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCCTCaa > 1:3637037/1‑68 (MQ=255) gCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAgaga > 1:112620/1‑68 (MQ=255) ccAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCCTCaa > 1:1842467/1‑63 (MQ=255) cAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGc < 1:1948170/71‑1 (MQ=255) tCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGa > 1:1587109/1‑71 (MQ=255) aGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGAtt < 1:248725/71‑1 (MQ=255) cGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGAt > 1:3462035/1‑66 (MQ=255) cGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGAt > 1:586812/1‑66 (MQ=255) cGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCg > 1:3222429/1‑71 (MQ=255) tAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCgg > 1:4115956/1‑70 (MQ=255) tAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCgg > 1:925600/1‑70 (MQ=255) tAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGc < 1:1419209/71‑1 (MQ=255) aaTTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATg < 1:1491643/61‑1 (MQ=255) ttCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAagag > 1:925808/1‑48 (MQ=255) tCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAag < 1:4436032/45‑1 (MQ=255) tCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACAt > 1:2633998/1‑71 (MQ=255) tCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACAt > 1:3878398/1‑71 (MQ=255) cGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACAt < 1:748440/70‑1 (MQ=255) tGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAgaga < 1:3559461/45‑1 (MQ=255) tGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGcc < 1:1219294/51‑1 (MQ=255) ccATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAaga > 1:4510693/1‑41 (MQ=255) tctcAGCAGTGAGCTTACGTCCGGCATGTCCTTCaa < 1:4036013/36‑1 (MQ=255) tctcAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAgaga < 1:3588784/40‑1 (MQ=255) tctcAGCAGTGAGCTTACGTCCGGCATGTCCCTCAag < 1:402274/37‑1 (MQ=255) tcAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGAt > 1:342822/1‑39 (MQ=255) tcAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGcc > 1:4425868/1‑44 (MQ=255) tcAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGc < 1:4391517/43‑1 (MQ=255) tcAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCaa > 1:2617282/1‑58 (MQ=255) cagcagTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTc > 1:1117113/1‑71 (MQ=255) cagcagTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTc > 1:4165139/1‑71 (MQ=255) agcagTGAGCTTACGTCCGGCATGTCCTTCAAGTGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCg < 1:1475849/71‑1 (MQ=255) tGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGc < 1:3335373/36‑1 (MQ=255) tGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGa < 1:3255343/41‑1 (MQ=255) tGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGc < 1:503967/67‑1 (MQ=255) aGCTTACGTCCGGCATGTCCCTCCAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGc < 1:989561/71‑1 (MQ=255) aGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTc > 1:766088/1‑63 (MQ=255) cTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGc < 1:3579049/63‑1 (MQ=255) tACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAggg > 1:1559114/1‑71 (MQ=255) gTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGgtgt < 1:3226314/71‑1 (MQ=255) gCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATATGCCGCTGTCGCATCTGCAGGGTGTTTTAc > 1:4054492/1‑71 (MQ=255) tCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTg > 1:1207933/1‑38 (MQ=255) tCCCTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGAc > 1:3857216/1‑71 (MQ=255) cTTCAAGAGATACGCCATGATTAGCGGCAACATCTGc > 1:4485464/1‑37 (MQ=255) cTTCAAGAGATACGCCATGATTAGCGGCAACATCTGc > 1:2780408/1‑37 (MQ=255) cTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTa > 1:3021472/1‑63 (MQ=255) ttCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCt < 1:3170293/40‑1 (MQ=255) ttCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGAc < 1:1371369/68‑1 (MQ=255) | ACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGAC > W3110S.gb/1044837‑1044971 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |