| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,093,686 | T→C | 100% | V130A (GTA→GCA) | ycdT → | predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,093,686 | 0 | T | C | 98.0% | 161.6 / ‑5.2 | 48 | V130A (GTA→GCA) | ycdT | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (22/25); minor base A (1/0); total (23/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACT > W3110S.gb/1093619‑1093747 | gtCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCAtt > 1:3038659/1‑71 (MQ=255)gtCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCAtt > 1:294042/1‑71 (MQ=255) tCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTg < 1:1932325/71‑1 (MQ=255) ccAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTgg > 1:1646163/1‑71 (MQ=255) cAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGAATATTAATATTTCTGGCATTGGt > 1:2998109/1‑71 (MQ=255) cATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATT‑GTGAATAAAGt < 1:3525481/71‑1 (MQ=255) gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGATAg > 1:1328028/1‑71 (MQ=255) cAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGt < 1:4122243/71‑1 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGa > 1:740970/1‑70 (MQ=255) cTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAg > 1:1585488/1‑62 (MQ=255) tattatTTGTTTCGTCAGATGAGTTTGTGTATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa > 1:288950/1‑71 (MQ=255) ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGt < 1:445060/60‑1 (MQ=255) ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa < 1:1928309/67‑1 (MQ=255) ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa < 1:4313957/67‑1 (MQ=255) ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa < 1:4465873/67‑1 (MQ=255) ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAaca > 1:3919441/1‑71 (MQ=255) tttGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:78162/71‑1 (MQ=255) tGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa > 1:2480179/1‑71 (MQ=255) tGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa > 1:3842781/1‑71 (MQ=255) gTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTa > 1:663995/1‑56 (MQ=255) gTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAg > 1:3475636/1‑52 (MQ=255) gTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaa < 1:4137583/57‑1 (MQ=255) gTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaa < 1:3619643/57‑1 (MQ=255) gTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaa < 1:3625119/57‑1 (MQ=255) tCAGATGAGTTTGTGCATATTAATATTTCTGGCAtt < 1:1376561/36‑1 (MQ=255) cAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAg > 1:1180650/1‑46 (MQ=255) cAGATGAGTTTGTGCATATTAATATTTCTGGAATTGGTGAATAAAGTTAGTGaaaa > 1:2955142/1‑56 (MQ=255) aTGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:2310436/58‑1 (MQ=255) aTGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattt < 1:2617569/71‑1 (MQ=255) aTGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattt < 1:4561982/71‑1 (MQ=255) aTGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattt < 1:3830083/71‑1 (MQ=255) gAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttat > 1:195368/1‑71 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTc > 1:3547817/1‑70 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTc > 1:159892/1‑70 (MQ=255) tatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:4482760/45‑1 (MQ=255) tatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:1217070/45‑1 (MQ=255) tatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGAGCAATTTATTTTCCa < 1:3802343/66‑1 (MQ=255) atTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa < 1:3794034/39‑1 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:134109/43‑1 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaa > 1:2692046/1‑69 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt > 1:3991442/1‑71 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt > 1:142114/1‑71 (MQ=255) tatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACt < 1:3501518/71‑1 (MQ=255) tttCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaa < 1:4253802/38‑1 (MQ=255) tttCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:667073/37‑1 (MQ=255) ttCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:243261/36‑1 (MQ=255) tGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttatt > 1:3037167/1‑49 (MQ=255) tGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttatt > 1:4592486/1‑49 (MQ=255) | GTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACT > W3110S.gb/1093619‑1093747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |