| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,122,815 | C→G | 29.6% | intergenic (‑283/+4) | yceP ← / ← dinI | hypothetical protein/DNA damage‑inducible protein I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,122,815 | 0 | C | G | 29.6% | 34.0 / 14.9 | 27 | intergenic (‑283/+4) | yceP/dinI | hypothetical protein/DNA damage‑inducible protein I |
| Reads supporting (aligned to +/- strand): ref base C (9/10); new base G (5/3); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
ATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTG > W3110S.gb/1122750‑1122884 | aTTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCatat > 1:1783654/1‑70 (MQ=255) tACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATATTATTCg < 1:3760240/70‑1 (MQ=255) cTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCAtatta > 1:211727/1‑64 (MQ=255) tCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCa < 1:4290686/58‑1 (MQ=255) aaaaTTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATATTATTCGCTGAc > 1:953273/1‑70 (MQ=255) aTTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATATTATTCGCTGACAAAc > 1:1318188/1‑71 (MQ=255) aaaataaaaAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCgg > 1:4634932/1‑71 (MQ=255) gctaaggaagctaaaatggagAAAAAAATCACTGGata < 1:535202‑M1/17‑1 (MQ=255) ctaaggaagctaaaatggagAAAAAAATCACTGGatat > 1:3742632‑M1/21‑38 (MQ=255) ctaaggaagctaaaatggagAAAAAAATCACTGGatat > 1:1678815‑M1/21‑38 (MQ=255) ctaaggaagctaaaatggagAAAAAAATCACTGGata > 1:4429769‑M1/21‑37 (MQ=255) taaggaagctaaaatggagAAAAAAATCACTGGata > 1:336797‑M1/20‑36 (MQ=255) aaggaagctaaaatggagAAAAAAATCACTGGata < 1:485887‑M1/17‑1 (MQ=255) aaggaagctaaaatggagAAAAAAATCACTGGata > 1:4098185‑M1/19‑35 (MQ=255) aaggaagctaaaatggagAAAAAAATCACTGGata < 1:2216175‑M1/17‑1 (MQ=255) ccGGGGCGACCCGGCAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTcc < 1:4089862/67‑1 (MQ=255) ccGGGGCGACCCGGCAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTc < 1:2769457/66‑1 (MQ=255) cGGGGCGACCCGGCAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGt > 1:4335612/1‑71 (MQ=255) gggCGACCCGGCAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCa < 1:4190329/66‑1 (MQ=255) ggCAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTc > 1:368316/1‑42 (MQ=255) gCAAAAAAAATCACTGCATATTATTCGCTGACaaa > 1:2434323/1‑35 (MQ=255) gCAAAAAAAATCACTGCATATTATTCGCTGACAAACCa > 1:3318882/1‑38 (MQ=255) aaaaaaTCACTGCATATTATTCGCTGACAAACCAGTc < 1:1536488/37‑1 (MQ=255) aaaaaTCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCAc < 1:4233098/71‑1 (MQ=255) aaaTCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCt < 1:3152785/43‑1 (MQ=255) aaaTCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACt < 1:4314933/70‑1 (MQ=255) aCTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGgag < 1:3499056/56‑1 (MQ=255) cATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTg > 1:2004404/1‑70 (MQ=255) | ATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTGGAGAATTTCACTAATGCGCTG > W3110S.gb/1122750‑1122884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |