| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,168,260 | G→A | 12.2% | G200D (GGT→GAT) | ndh → | respiratory NADH dehydrogenase 2/cupric reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,168,260 | 0 | G | A | 12.2% | 137.2 / 8.5 | 49 | G200D (GGT→GAT) | ndh | respiratory NADH dehydrogenase 2/cupric reductase |
| Reads supporting (aligned to +/- strand): ref base G (19/24); new base A (4/2); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTATTTTGCC > W3110S.gb/1168194‑1168326 | gcggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTAc < 1:2558807/71‑1 (MQ=255) cggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACa < 1:2798749/71‑1 (MQ=255) cggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACa < 1:23045/71‑1 (MQ=255) aCGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACaaa > 1:2314145/1‑66 (MQ=255) aCGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACaaa > 1:2386381/1‑66 (MQ=255) aCGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACa < 1:3941255/64‑1 (MQ=255) aCGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACa < 1:374204/64‑1 (MQ=255) cGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTg < 1:123958/71‑1 (MQ=255) gtAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCaa > 1:794677/1‑71 (MQ=255) tAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAAc > 1:3546985/1‑71 (MQ=255) tAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGATTACAAAGGCCTGACCAAc > 1:709604/1‑71 (MQ=255) tAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGATTACAAAGGCCTGACCAAc > 1:2535646/1‑71 (MQ=255) tAGAACTCTCCGATGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAAc > 1:3470143/1‑71 (MQ=255) ctcCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACg > 1:3789335/1‑65 (MQ=255) ctcCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGccc > 1:3268025/1‑71 (MQ=255) tcCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCaa > 1:688697/1‑62 (MQ=255) tcCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCaa > 1:1982776/1‑62 (MQ=255) tcCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCaa > 1:1546599/1‑62 (MQ=255) aaTTGCACAACGCGGTCAAGCAACTGCACAGCTACGATTACaaa < 1:1885356/44‑1 (MQ=255) gCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGc > 1:3304352/1‑71 (MQ=255) acaACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCt < 1:1618385/70‑1 (MQ=255) acaACGCGGTCAAGCAACTGCACAGCTACGATTACAAAGGCCTGACCAACGAAGCCCTGAACGt < 1:715743/64‑1 (MQ=255) caACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTgg < 1:615158/71‑1 (MQ=255) aaCGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGt < 1:1833744/62‑1 (MQ=255) aaCGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAACCCCTGa < 1:632985/58‑1 (MQ=255) ggTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAg < 1:2551721/71‑1 (MQ=255) gTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGc < 1:664213/47‑1 (MQ=255) gTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGc < 1:4245221/61‑1 (MQ=255) tCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGa < 1:941664/67‑1 (MQ=255) tCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGa < 1:668785/67‑1 (MQ=255) cAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTg > 1:2120601/1‑61 (MQ=255) gCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTg < 1:958775/46‑1 (MQ=255) gCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTg < 1:964078/46‑1 (MQ=255) gCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTg < 1:3895151/46‑1 (MQ=255) aaCTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCgg > 1:3437397/1‑66 (MQ=255) cTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACg < 1:3398125/71‑1 (MQ=255) tGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACg > 1:2916793/1‑70 (MQ=255) gCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTa > 1:3050278/1‑71 (MQ=255) gCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTa > 1:2067012/1‑71 (MQ=255) cacaGCTACGGTTACAAAGGCCTGACCAACGACCCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTAt > 1:475975/1‑71 (MQ=255) cacaGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGaa > 1:2394186/1‑41 (MQ=255) cacaGCTACGATTACAAAGGCCTGACCAACGAAGCCCTGAAc > 1:2917233/1‑42 (MQ=255) cacaGCTACGATTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTAt > 1:1983823/1‑71 (MQ=255) acaGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTAtt < 1:589135/71‑1 (MQ=255) atcTACGGGTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGgaga < 1:4087511/60‑1 (MQ=255) aGCTACGGTTACAAAGGCCTGACCAACGAATCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTAtttt < 1:3053831/71‑1 (MQ=255) aGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTTAACGTAACGCTGGTAGAAGCGGGAGAACGTAtttt < 1:2326635/71‑1 (MQ=255) cTACGGTTACAAAGCCCTGACCAACGAAGCCCTGAACGTAACGCTGTTAGAAGCGGGAGAACGTATTTTGc < 1:2882361/71‑1 (MQ=255) tACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTATTTTGcc > 1:3776959/1‑71 (MQ=255) | GCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTATTTTGCC > W3110S.gb/1168194‑1168326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |