| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,280,527 | A→G | 17.6% | S332G (AGC→GGC) | narK → | nitrate/nitrite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,280,527 | 0 | A | G | 17.6% | 74.5 / 10.4 | 34 | S332G (AGC→GGC) | narK | nitrate/nitrite transporter |
| Reads supporting (aligned to +/- strand): ref base A (15/13); new base G (2/4); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCG > W3110S.gb/1280460‑1280583 | tgCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCg < 1:1794123/71‑1 (MQ=255) aTCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCGGCGGCctgc > 1:4030064/1‑71 (MQ=255) aTCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCGGCGGCctgc > 1:2510106/1‑71 (MQ=255) aTCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCctgc > 1:2724990/1‑71 (MQ=255) aTCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCctgc > 1:4023347/1‑71 (MQ=255) gACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGtt > 1:4066810/1‑71 (MQ=255) aCCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGt < 1:1333968/69‑1 (MQ=255) cGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCGGCGGCCTGCTGTTCCt < 1:2669483/71‑1 (MQ=255) gTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTg < 1:3133584/71‑1 (MQ=255) tGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTg < 1:2391074/68‑1 (MQ=255) ggCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCGGCGGCCTGCTGTTCCTGACCtt < 1:2205856/71‑1 (MQ=255) ggCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCg > 1:3208270/1‑52 (MQ=255) cGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCctgctg < 1:1882849/58‑1 (MQ=255) cGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTAc > 1:196762/1‑71 (MQ=255) gAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGa < 1:363641/63‑1 (MQ=255) tCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGa < 1:2396740/59‑1 (MQ=255) tCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACt > 1:299056/1‑70 (MQ=255) tCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACt > 1:1814012/1‑70 (MQ=255) tgtCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACg < 1:1361466/71‑1 (MQ=255) gtCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACgg > 1:3003538/1‑71 (MQ=255) tCACGCTGGTGAACTTTATTCTGATGGCGATTTTCGGCGGCCTGCTGTTCCTg < 1:553423/53‑1 (MQ=255) tCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTa > 1:1893778/1‑59 (MQ=255) gTGAACTTTATTCTGATGGCGATTTTCGGCGGCctgc < 1:2992899/37‑1 (MQ=255) gTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTAcc < 1:595080/53‑1 (MQ=255) gTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACggg < 1:2531351/63‑1 (MQ=255) gAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCa > 1:3070920/1‑63 (MQ=255) tttATTCTGATGGCGATTTTCAGCGGCCTGCTGtt < 1:945874/35‑1 (MQ=255) tttATTCTGATGGCGATTTTCAGCGGCCTGCTGtt < 1:4490501/35‑1 (MQ=255) ttATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCa > 1:807178/1‑58 (MQ=255) ttATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCtt > 1:1443538/1‑70 (MQ=255) cTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTATCGACTGACGGGCAGGGCGGAAGCTTCATGGc > 1:3129800/1‑71 (MQ=255) cTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGc > 1:2061618/1‑71 (MQ=255) tGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTa > 1:1207136/1‑38 (MQ=255) aTGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCg < 1:3943518/69‑1 (MQ=255) | TGCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCG > W3110S.gb/1280460‑1280583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |