| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,366,654 | T→C | 25.2% | Y237H (TAT→CAT) | puuB → | gamma‑Glu‑putrescine oxidase, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,366,654 | 0 | T | C | 25.2% | 68.0 / 21.5 | 41 | Y237H (TAT→CAT) | puuB | gamma‑Glu‑putrescine oxidase, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (12/18); new base C (4/6); total (17/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCA > W3110S.gb/1366597‑1366723 | gtgCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATa > 1:4326460/1‑70 (MQ=255)gtgCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATa > 1:2620182/1‑70 (MQ=255) ccAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAg < 1:2004704/63‑1 (MQ=255) ccAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGCATCTGGGCGATAAAGTAGAGCCg > 1:3928794/1‑71 (MQ=255) cAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCgg < 1:4044004/71‑1 (MQ=255) aaaGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATaaa < 1:1055092/60‑1 (MQ=255) aaaGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTa > 1:214589/1‑63 (MQ=255) aaaGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGCATCTGGGCGATAAAGt > 1:1078920/1‑62 (MQ=255) aaaGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGCATCTGGGCGATAAAGTAGAGCCGGa < 1:2950326/71‑1 (MQ=255) aaGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGaa < 1:3661253/71‑1 (MQ=255) ggTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAAc > 1:4527151/1‑70 (MQ=255) ggTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACt > 1:2150436/1‑71 (MQ=255) gTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATaaa > 1:2686534/1‑51 (MQ=255) gTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTg < 1:3974823/66‑1 (MQ=255) gTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGCATCTGGGCGa < 1:1950540/47‑1 (MQ=255) tGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGa > 1:1234433/1‑46 (MQ=255) tGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGaaa > 1:1331120/1‑71 (MQ=255) aCGGCGAAGTATGTGATTGTCGCCGGGAATGCGCATCTGGGCGATAAAGTagag > 1:991145/1‑54 (MQ=255) ggCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATaa > 1:258730/1‑45 (MQ=255) ggCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACg > 1:1571179/1‑69 (MQ=255) gCGAAGTATGTGATTGTCGCCGGGAATGCGCATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACgcag < 1:4049304/71‑1 (MQ=255) gAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACgcagca < 1:2370562/71‑1 (MQ=255) gTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCg < 1:149884/49‑1 (MQ=255) tgtgATTGTCGCCGGGAATGCGTATCTGGGCGATAAAg < 1:139410/38‑1 (MQ=255) tgATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTgg > 1:1540965/1‑51 (MQ=255) tgATTGTCGCCGGGAATGCGCATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGc > 1:2352839/1‑71 (MQ=255) gATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACgcag < 1:3333691/60‑1 (MQ=255) gATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACgcag < 1:3780097/60‑1 (MQ=255) gATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACgcag < 1:543344/60‑1 (MQ=255) aTTGTCGCCGGGAATGCGTATCTGGGCGATAAAGt < 1:4150580/35‑1 (MQ=255) aTTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCgg < 1:4570969/43‑1 (MQ=255) aTTGTCGCCGGGAATGCGCATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCgg < 1:3556586/71‑1 (MQ=255) aTTGTCGCCGGGAATGCGAATCTGGGCGATAAAGTa > 1:4449933/1‑36 (MQ=255) gCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGc < 1:2385332/70‑1 (MQ=255) gCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCa < 1:355924/71‑1 (MQ=255) gAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGa < 1:4101401/71‑1 (MQ=255) aTGCGCATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCgg < 1:1911991/58‑1 (MQ=255) aTGCGCATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGAt < 1:1889229/70‑1 (MQ=255) gcgcATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACgcag < 1:3408324/44‑1 (MQ=255) gCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCActac < 1:3821369/62‑4 (MQ=255) cGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATcac > 1:3495054/1‑70 (MQ=255) gTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATcacca < 1:1435921/71‑1 (MQ=255) | GTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCA > W3110S.gb/1366597‑1366723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |