| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,367,159 | C→T | 29.6% | T405M (ACG→ATG) | puuB → | gamma‑Glu‑putrescine oxidase, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,367,159 | 0 | C | T | 29.6% | 35.7 / 17.4 | 27 | T405M (ACG→ATG) | puuB | gamma‑Glu‑putrescine oxidase, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (7/12); new base T (5/3); total (12/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
CGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATT > W3110S.gb/1367092‑1367228 | cgcgGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCATGCt > 1:2525311/1‑71 (MQ=255) cggctACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGc < 1:1356827/71‑1 (MQ=255) cggcgACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGc < 1:932635/71‑1 (MQ=255) gCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGcc > 1:643816/1‑71 (MQ=255) gAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCATGCTGCGTGTGCCGtt > 1:4498731/1‑71 (MQ=255) aaCGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGtt > 1:2204493/1‑70 (MQ=255) tCGATGCCTTCGCCAATCTGCCGCATTCCCCGTTCCCCGGCGGGCGCTCGCTGCGTGTGCCGTTTACcgc > 1:3056831/1‑70 (MQ=255) tCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACcgc < 1:801695/70‑1 (MQ=255) tCGATGCCTTCGCCAATCTGACGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACcgc < 1:3370421/70‑1 (MQ=255) cGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCATGCTGCGTGTGCCGTTTACcgcg < 1:1241647/70‑1 (MQ=255) gATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGAt < 1:1739831/71‑1 (MQ=255) ccTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACTCTGCGTGTGCCGTTTACCGCGATggg < 1:3639511/70‑1 (MQ=255) ttCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCATGCTGCGTGTGCCGt < 1:357380/55‑1 (MQ=255) gCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGta > 1:122827/1‑52 (MQ=255) aaTCTGCCGCATTACCCGTTCCCCGGCGGGCGCATGCTGCGTGTGCCGTTTAc > 1:4466133/1‑53 (MQ=255) tGCCGCATTACCCGTTCCCCGGCGGGCGCATGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTa > 1:1567422/1‑70 (MQ=255) tGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTa > 1:2953295/1‑70 (MQ=255) ccGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTAtt < 1:4050690/67‑1 (MQ=255) ccGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTAtt < 1:2951304/67‑1 (MQ=255) ccGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACa < 1:1752434/70‑1 (MQ=255) ccGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCgcggcg < 1:3396994/54‑1 (MQ=255) ccccGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCg < 1:939785/69‑1 (MQ=255) ccGGCGGGCGCATGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTg < 1:3936342/71‑1 (MQ=255) ggcgggcgCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTggg > 1:95544/1‑71 (MQ=255) ggcgggcgCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTggg > 1:101023/1‑71 (MQ=255) cgcATGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGttt > 1:1423925/1‑70 (MQ=255) aCGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAAtt < 1:3195427/71‑1 (MQ=255) | CGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATT > W3110S.gb/1367092‑1367228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |