| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,389,988 | C→T | 14.3% | intergenic (+13/+31) | tyrR → / ← tpx | DNA‑binding transcriptional dual regulator, tyrosine‑binding/lipid hydroperoxide peroxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,389,988 | 0 | C | T | 14.3% | 88.7 / 7.3 | 35 | intergenic (+13/+31) | tyrR/tpx | DNA‑binding transcriptional dual regulator, tyrosine‑binding/lipid hydroperoxide peroxidase |
| Reads supporting (aligned to +/- strand): ref base C (17/13); new base T (2/3); total (19/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
CGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCC > W3110S.gb/1389918‑1390055 | cgcgATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGcc > 1:3068611/1‑71 (MQ=255) cgATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCt > 1:2936939/1‑70 (MQ=255) aTTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGAt > 1:4124177/1‑71 (MQ=255) aTTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGAt > 1:1221201/1‑71 (MQ=255) tAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCaa > 1:2043273/1‑70 (MQ=255) gTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGAt < 1:227494/60‑1 (MQ=255) gCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCAt > 1:1647598/1‑70 (MQ=255) cGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATc < 1:3446954/70‑1 (MQ=255) gAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCTTGATGGTGCaa > 1:4436559/1‑60 (MQ=255) gAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGc < 1:2567056/71‑1 (MQ=255) gTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAAt < 1:184358/67‑1 (MQ=255) tCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATg < 1:3667842/70‑1 (MQ=255) agaagaagaACGAAGAGTAAGCGCGAATATGCTTGAt < 1:4169653/37‑1 (MQ=255) agaagaagaACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTa < 1:3077303/61‑1 (MQ=255) agaagaagaACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCtt > 1:3449053/1‑71 (MQ=255) gaagaagaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCttt > 1:1003435/1‑71 (MQ=255) agaagaaCGAAGAGTAAGCGCGAATATGCTTGATg < 1:267205/35‑1 (MQ=255) gaagaaCGAAGAGTAAGCGCGAATATGCTTGATGGTGCAACACCATCAGGCATATTAAATTa > 1:505075/1‑62 (MQ=255) gaagaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTa > 1:677507/1‑62 (MQ=255) aagaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGc < 1:3371415/37‑1 (MQ=255) gaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGc < 1:477111/35‑1 (MQ=255) aCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCa > 1:1289249/1‑43 (MQ=255) gagTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCaga < 1:3659179/71‑1 (MQ=255) gTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAAtt > 1:780471/1‑49 (MQ=255) gTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgc > 1:4253870/1‑71 (MQ=255) gTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgc > 1:2413791/1‑71 (MQ=255) gTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgc > 1:910222/1‑71 (MQ=255) aGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCa > 1:2372703/1‑55 (MQ=255) cgcgAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTa < 1:3122972/56‑1 (MQ=255) gcgAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCaga > 1:1071833/1‑63 (MQ=255) gAATATGCTTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCg < 1:3006592/70‑1 (MQ=255) gAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGt < 1:3243709/71‑1 (MQ=255) tatGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAAt > 1:896650/1‑71 (MQ=255) tGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATcc < 1:2720283/71‑1 (MQ=255) tGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATcc > 1:2215607/1‑71 (MQ=255) tGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATcc < 1:987593/71‑1 (MQ=255) cTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCag < 1:4271266/52‑1 (MQ=255) cTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCag < 1:2899814/52‑1 (MQ=255) | CGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCC > W3110S.gb/1389918‑1390055 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |