| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,408,005 | T→C | 8.3% | F105L (TTT→CTT) | ydaL → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,408,005 | 0 | T | C | 8.3% | 122.2 / 4.0 | 48 | F105L (TTT→CTT) | ydaL | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (13/31); new base C (2/2); total (15/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
CGCAACAGGCGAGCCTGAATCTTTTGCGCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGATTATTCATGGTAAAGGGCGGGATGATA > W3110S.gb/1407940‑1408074 | cGCAACAGGCGAGCCTGAATCTTTTGCGCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTAtt > 1:1454287/1‑71 (MQ=255) gCAACAGGCGAGCCTGAATCTTTTGCGCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTc < 1:2935673/71‑1 (MQ=255) cAACAGGCGAGCCTGAATCTTTTGCGCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTc > 1:1053553/1‑70 (MQ=255) aaCAGGCGAGCCTGAATCTTTTGCGCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTcaa < 1:2944254/71‑1 (MQ=255) aaCAGGCGAGCCTGAATCTTTTGCGCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTcaa < 1:4290942/71‑1 (MQ=255) ggCGAGCCTGAATCTTTTGCGCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAg > 1:348113/1‑71 (MQ=255) gAATCTTTTGCGCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGcc > 1:4001936/1‑64 (MQ=255) tCTTTTGCTCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTCTTATTcaa > 1:4133566/1‑55 (MQ=255) tCTTTTGCGCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAg < 1:3295257/59‑1 (MQ=255) tCTTTTGCGCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTCTTATTcaa > 1:3330721/1‑55 (MQ=255) cTTTTGCGCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGGTCAGTTTTATTcaacaa < 1:4085376/57‑1 (MQ=255) ttttGCGCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGt > 1:1739162/1‑71 (MQ=255) tttGCGCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGtt > 1:1819581/1‑71 (MQ=255) tGCGCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTg > 1:614119/1‑71 (MQ=255) tGCGCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTg > 1:1447789/1‑71 (MQ=255) cgcCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGc < 1:86715/70‑1 (MQ=255) ccAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTa < 1:463659/71‑1 (MQ=255) cAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTaa > 1:1798448/1‑71 (MQ=255) aGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAAc < 1:4129496/71‑1 (MQ=255) gCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACg < 1:682860/71‑1 (MQ=255) gTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGa < 1:3616289/53‑1 (MQ=255) tGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTcaac < 1:172836/39‑1 (MQ=255) tGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTcaac < 1:1331632/39‑1 (MQ=255) gAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTcaa < 1:2718810/36‑1 (MQ=255) gAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATgg < 1:68127/53‑1 (MQ=255) agagTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGatta < 1:960043/71‑1 (MQ=255) agagTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGatta < 1:1342313/71‑1 (MQ=255) agagTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGatta < 1:1461787/71‑1 (MQ=255) gagTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGattat < 1:463844/71‑1 (MQ=255) gagTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGattat < 1:1874373/71‑1 (MQ=255) gTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGattatt > 1:2460664/1‑70 (MQ=255) tGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATgg < 1:433625/47‑1 (MQ=255) ccGCAAAATGGTGTTCAGTTTTATTAAACAAGCCCTGGCGGATgg > 1:3240487/1‑45 (MQ=255) aaaaTGGTGTTCAGTTTTATTCAACAAGCCATGGCGGATGGTTTGCGTAACGTGCTGATTATTCATGGTaa < 1:1250551/71‑1 (MQ=255) aaaTGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACg > 1:3587815/1‑51 (MQ=255) aaTGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGt < 1:2574830/46‑1 (MQ=255) aTGGTGTTCAGTTTTAATCAACAAGCCCTGGCGGATgg > 1:3795558/1‑38 (MQ=255) ttCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCg < 1:3989242/38‑1 (MQ=255) ttCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGat < 1:1824505/50‑1 (MQ=255) ttCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGATTATTCATTGTAAAGGGCggg < 1:4439761/70‑1 (MQ=255) ttCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGATTATTCATGGTaa < 1:4358003/62‑1 (MQ=255) ttCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGATTATTCATGGTAAAGGGCggg < 1:4541267/70‑1 (MQ=255) ttCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGATTATTCATGGTAAAGGGCggg < 1:670525/70‑1 (MQ=255) ttCAGTCTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGATTATTCAt < 1:801430/57‑1 (MQ=255) ttCAGTCTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGATTATTCAt < 1:4227287/57‑1 (MQ=255) gTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGat < 1:2957803/46‑1 (MQ=255) gTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGat < 1:1398585/46‑1 (MQ=255) gTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGat < 1:924824/46‑1 (MQ=255) ttttATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGATTATTCATGGt < 1:1384718/55‑1 (MQ=255) ttttATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGATTATTCATGGTa < 1:687813/56‑1 (MQ=255) ttttATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGATTATTCATGGTAAAGGGCGGGATGATa < 1:1518974/71‑1 (MQ=255) ttttATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGATTATTCATGGTAAAGGGCGGGATGATa < 1:3285293/71‑1 (MQ=255) | CGCAACAGGCGAGCCTGAATCTTTTGCGCCAGCCGGTGGAAGAGTGCCGCAAAATGGTGTTCAGTTTTATTCAACAAGCCCTGGCGGATGGTTTGCGTAACGTGCTGATTATTCATGGTAAAGGGCGGGATGATA > W3110S.gb/1407940‑1408074 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |