| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,413,273 | G→T | 12.7% | R130R (CGC→CGA) | ydaO ← | predicted C32 tRNA thiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,413,273 | 0 | G | T | 12.7% | 142.5 / 5.4 | 47 | R130R (CGC→CGA) | ydaO | predicted C32 tRNA thiolase |
| Reads supporting (aligned to +/- strand): ref base G (20/21); new base T (3/3); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGTAAG > W3110S.gb/1413207‑1413343 | gTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgac > 1:3033450/1‑71 (MQ=255) tCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgacg < 1:1798715/71‑1 (MQ=255) tGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgacg < 1:1928264/65‑1 (MQ=255) gTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCgag < 1:1600375/71‑1 (MQ=255) gTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCgag < 1:945838/71‑1 (MQ=255) aCCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGaa > 1:70598/1‑70 (MQ=255) ccAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAacac < 1:4484902/71‑1 (MQ=255) ccAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAaca > 1:3202158/1‑70 (MQ=255) aCGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGt > 1:2279405/1‑71 (MQ=255) cgcgATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTg < 1:543059/71‑1 (MQ=255) cgcgATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTg < 1:3066636/71‑1 (MQ=255) cgcgATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTg < 1:1515250/71‑1 (MQ=255) tCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAacac > 1:2358137/1‑62 (MQ=255) ttCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAg > 1:1551961/1‑62 (MQ=255) ttCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGt > 1:4208251/1‑71 (MQ=255) cgtcgCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCTCGACGAAGGCGAGAACACAGTGAGc < 1:1340863/65‑1 (MQ=255) cgtcgCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGc < 1:2225862/65‑1 (MQ=255) gCCCCCAGTTCCGTTGCTGTACGATAAAGGATACCGCGACGAAGGCGAGaa < 1:2475242/51‑1 (MQ=255) cccccAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg < 1:3354864/71‑1 (MQ=255) ccAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGtttt < 1:296429/67‑1 (MQ=255) cAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCct > 1:1162683/1‑71 (MQ=255) aGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCctc > 1:2279001/1‑71 (MQ=255) aGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCct > 1:1772848/1‑70 (MQ=255) aGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAACTGGTTTTGCCctc > 1:2272187/1‑71 (MQ=255) ccGTTGCGGTACGATAAAGGATACCTCGACGAAGGCGAGAACACAGTGAGCAAGTgg > 1:4549506/1‑57 (MQ=255) ccGTTGCGGTACGATAAAGGATACCTCGACGAAGGCGAGAACACAGTGAGCAAGTgg > 1:320398/1‑57 (MQ=255) ccGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTgg > 1:329467/1‑70 (MQ=255) ttGCGGTACGATAAAGGATACCTCGACGAAGGCGAGAACACAGTGAGCAAGTgg < 1:647407/54‑1 (MQ=255) ttGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGt < 1:3076121/55‑1 (MQ=255) ttGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGt < 1:1614306/55‑1 (MQ=255) ttGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGt < 1:3960098/55‑1 (MQ=255) ttGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGaa < 1:2386349/69‑1 (MQ=255) gCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCct > 1:3195907/1‑61 (MQ=255) gTACGATAAAGGATACCTCGACGAAGGCGAGAACACAGTGAGCaa > 1:570091/1‑45 (MQ=255) gTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGc < 1:271462/43‑1 (MQ=255) aCGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtctt > 1:953561/1‑66 (MQ=255) aCGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTGTCtt > 1:707637/1‑71 (MQ=255) cGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCctct > 1:2790963/1‑57 (MQ=255) cGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTgg < 1:4625787/59‑1 (MQ=255) gATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg < 1:3772953/50‑1 (MQ=255) aTAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtctt > 1:236077/1‑63 (MQ=255) aaaGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCAc > 1:886894/1‑70 (MQ=255) aaGGATACCTCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGa < 1:2667686/71‑1 (MQ=255) aaGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGt < 1:4181378/39‑1 (MQ=255) ccGCTACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGt < 1:1891809/70‑1 (MQ=255) ccGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAt > 1:1118840/1‑50 (MQ=255) ccGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGt > 1:1976609/1‑70 (MQ=255) gcgACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGTAAg > 1:2981771/1‑71 (MQ=255) | GTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCACGATACCGTAAG > W3110S.gb/1413207‑1413343 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |