| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,438,971 | C→T | 25.0% | intergenic (‑364/+3) | ompN ← / ← ydbK | outer membrane pore protein N, non‑specific/fused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,438,971 | 0 | C | T | 25.0% | 40.1 / 11.2 | 24 | intergenic (‑364/+3) | ompN/ydbK | outer membrane pore protein N, non‑specific/fused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (5/13); new base T (3/3); total (8/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
ATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTT > W3110S.gb/1438903‑1439034 | atatAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCAt < 1:2058861/71‑1 (MQ=255) aaGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTTTCCAGAGc > 1:472899/1‑62 (MQ=255) aGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTcc < 1:3976472/71‑1 (MQ=255) gtcCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGTATTTAAt > 1:3756667/1‑50 (MQ=255) cGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCtttt < 1:2403205/70‑1 (MQ=255) cGGGCTTTTTTTTTAGAATTGGATAATACTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTc < 1:2325568/71‑1 (MQ=255) gCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGTATTTAATCGGTGTTGCt < 1:3107639/54‑1 (MQ=255) gCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCgg > 1:3577393/1‑71 (MQ=255) tttttttttAGAATTGGATAATCCTTATCCAGAGTATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGc > 1:3361777/1‑70 (MQ=255) tttttttttAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTc < 1:4117782/66‑1 (MQ=255) ttttttAGAATTGGATAATCCTTATCCAGAGCATTTAATCgg < 1:492887/42‑1 (MQ=255) ttttttAGAATTGGATAATCCTTATCCAGAGCATTTAATCgg < 1:355895/42‑1 (MQ=255) ttttttAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCAt < 1:2295703/70‑1 (MQ=255) tttttAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCAttt > 1:2238608/1‑71 (MQ=255) tAGAATTGGATAATCCTTATCCAGAGTATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTtgtg < 1:4333580/70‑1 (MQ=255) tAGAATTGGATAATCCTTATCCAGAGTATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTtgt < 1:2739239/69‑1 (MQ=255) ttGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAgg > 1:2049409/1‑70 (MQ=255) tGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCtttt < 1:1593293/41‑1 (MQ=255) ggATAATCCTTATCCAGAGTATTTAATCGGTGTTGCTTTTTTc > 1:3401352/1‑43 (MQ=255) gATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGt < 1:4548981/71‑1 (MQ=255) aTCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCAt < 1:896487/70‑1 (MQ=255) tCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGcc < 1:1000270/50‑1 (MQ=255) ccTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGc > 1:3505399/1‑48 (MQ=255) tATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGtt > 1:1569621/1‑71 (MQ=255) tCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGtttt < 1:3667124/71‑1 (MQ=255) | ATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTT > W3110S.gb/1438903‑1439034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |