| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,473,932 | G→T | 11.9% | pseudogene (6827/8622 nt) pseudogene (1529/3324 nt) |
ydbA → ydbA → |
ECK1398:JW5802+JW1402:b4492; predicted outer membrane protein ECK1398:JW1402:b1405; predicted outer membrane protein, C‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,473,932 | 0 | G | T | 11.9% | 235.2 / 10.7 | 75 | pseudogene (6827/8622 nt) pseudogene (1529/3324 nt) | ydbA ydbA | ECK1398:JW5802+JW1402:b4492; predicted outer membrane protein ECK1398:JW1402:b1405; predicted outer membrane protein, C‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base G (27/39); new base T (5/4); total (32/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
CCGGCGATCAGAATGCCTTCTTCTGGAACCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATGAACATCAGCGGCACCGG > W3110S.gb/1473863‑1474001 | ccGGCGATCAGAATGCCTTCTTCTGGAACCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAg < 1:3912644/70‑1 (MQ=255) cGGCGATCAGAATGCCTTCTTCTGGAACCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTg < 1:3703059/71‑1 (MQ=255) tCAGAATGCCTTCTTCTGGAACCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGa < 1:1874549/71‑1 (MQ=255) cAGAATGCCTTCTTCTGGAACCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGa > 1:1174596/1‑70 (MQ=255) cAGAATGCCTTCTTCTGGAACCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGa > 1:2263089/1‑70 (MQ=255) cAGAATGCCTTCTTCTGGAACCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGa > 1:1429402/1‑70 (MQ=255) aGAATGCCTTCTTCTGGAACCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGaaa < 1:609317/71‑1 (MQ=255) aaTGCCTTCTTCTGGAACCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGaa > 1:1310041/1‑68 (MQ=255) ttcttcTGGAACCATGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCa > 1:4298348/1‑67 (MQ=255) ttcttcTGGAACCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCa > 1:2451214/1‑67 (MQ=255) tcttctGGAACCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCa < 1:2672359/66‑1 (MQ=255) cttctGGAACCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAg > 1:4432631/1‑51 (MQ=255) cttctGGAACCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAg > 1:4010210/1‑51 (MQ=255) cttctGGAACCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCATTGCCGTGAAAGTCACCca < 1:4309581/69‑1 (MQ=255) tGGAACCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGaa > 1:4008917/1‑56 (MQ=255) aaCCATGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACtt < 1:3624/71‑1 (MQ=255) aaCCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACtt < 1:4169913/71‑1 (MQ=255) ccAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTa < 1:4288761/71‑1 (MQ=255) aGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCATTGCCg > 1:3090656/1‑45 (MQ=255) aGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCATTGCCGTGAAAGTCACCCACAGCAACTTTAtt > 1:985746/1‑71 (MQ=255) aGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTAtt > 1:3361355/1‑71 (MQ=255) gATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACtt < 1:1559293/65‑1 (MQ=255) gATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGc < 1:34745/71‑1 (MQ=255) aTAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGcc < 1:200935/71‑1 (MQ=255) aCGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACtt < 1:761168/61‑1 (MQ=255) ggggTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCAcc > 1:848157/1‑48 (MQ=255) gggTCATCAACTTCGATCACGACAGCGCCATTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAAt > 1:3466501/1‑71 (MQ=255) gggTCATCAACTTCGATCACGACAGCGCCATTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAAt > 1:319067/1‑71 (MQ=255) gggTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAAt < 1:2701054/71‑1 (MQ=255) tcatcaACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGc < 1:1959334/61‑1 (MQ=255) tcatcaACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCa < 1:917331/64‑1 (MQ=255) tcatcaACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCa < 1:1720668/64‑1 (MQ=255) tcatcaACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAg < 1:2528368/65‑1 (MQ=255) tcatcaACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGAc > 1:142595/1‑71 (MQ=255) atcaACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACgg > 1:2283822/1‑71 (MQ=255) tcaACTTCGATCACGACAGCGCCATTGCCGTGAAAGTCACCcac < 1:3322763/44‑1 (MQ=255) tcaACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCa < 1:1407306/61‑1 (MQ=255) caACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTa > 1:1748812/1‑53 (MQ=255) caACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGa > 1:1123529/1‑66 (MQ=255) aaCTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCaa > 1:3227372/1‑47 (MQ=255) aaCTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACtt < 1:2279918/50‑1 (MQ=255) aaCTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACtt < 1:3100475/50‑1 (MQ=255) aaCTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAg < 1:835402/60‑1 (MQ=255) aaCTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGaa > 1:1655971/1‑62 (MQ=255) aaCTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGcat > 1:1238990/1‑71 (MQ=255) cTTCGATCACGACAGCGCCATTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAg < 1:1026306/58‑1 (MQ=255) cTTCGATCACGACAGCGCCATTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAg < 1:3295499/58‑1 (MQ=255) cTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAAc < 1:3019225/46‑1 (MQ=255) cTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAAc < 1:4086919/46‑1 (MQ=255) ttCGATCACGACAGCGCCAGTGCCGTGAAAGTCAcc < 1:228414/36‑1 (MQ=255) ttCGATCACGACAGCGCCAGTGCCGTGAAAGTCAcc < 1:3346143/36‑1 (MQ=255) tCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGcatcat > 1:4561494/1‑70 (MQ=255) tCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGcatcat > 1:3647128/1‑70 (MQ=255) tCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGcatcat > 1:4407615/1‑70 (MQ=255) cGATCACGACAGCGCCATTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATg > 1:2301074/1‑70 (MQ=255) cGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTTTTGCCCAGAATGACGGCATCATg < 1:4032640/70‑1 (MQ=255) cGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATg < 1:2002606/70‑1 (MQ=255) cGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATg < 1:3796949/70‑1 (MQ=255) cGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATg < 1:1230268/70‑1 (MQ=255) cGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATg < 1:168964/70‑1 (MQ=255) aTCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTAtt < 1:3718815/47‑1 (MQ=255) tCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAAt < 1:3778356/55‑1 (MQ=255) tCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATGAACa < 1:1191780/71‑1 (MQ=255) cACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATGAAc > 1:1776277/1‑69 (MQ=255) cACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATGAACAt > 1:2330555/1‑71 (MQ=255) cACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATGAACAt > 1:4393471/1‑71 (MQ=255) cACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATGAACAt > 1:1263196/1‑71 (MQ=255) aCGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACttt < 1:3103325/41‑1 (MQ=255) aCGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATg < 1:294207/65‑1 (MQ=255) aCGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATGAACATc < 1:1042954/71‑1 (MQ=255) cAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATGAACATCAGCg > 1:4083851/1‑71 (MQ=255) aGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATGAACATCAGCgg < 1:1149150/71‑1 (MQ=255) aGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATGAACATCAGCgg > 1:928157/1‑71 (MQ=255) gcgcCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATGAACATCAGCGGc < 1:3031984/71‑1 (MQ=255) gcgcCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATGAACATCAGCGGc < 1:265471/71‑1 (MQ=255) gcCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATGAACATCAg > 1:4073844/1‑65 (MQ=255) cAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATGAACACCAGCGGCACCg > 1:4133456/1‑71 (MQ=255) aGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATGAACATCAGCGGCACCgg < 1:2346090/71‑1 (MQ=255) gTGCCGTGAAAGTCACCCCCAGCAACTTTATTGCCCAGaa > 1:1266769/1‑40 (MQ=255) gTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGaa > 1:88506/1‑40 (MQ=255) gTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGaa > 1:901270/1‑40 (MQ=255) gTGCCGTGAAAGTCACCCACAGCAACTTTATCGCCCAg < 1:8556/38‑1 (MQ=255) | CCGGCGATCAGAATGCCTTCTTCTGGAACCAGGATAACGGGGTCATCAACTTCGATCACGACAGCGCCAGTGCCGTGAAAGTCACCCACAGCAACTTTATTGCCCAGAATGACGGCATCATGAACATCAGCGGCACCGG > W3110S.gb/1473863‑1474001 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |