| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,487,609 | C→T | 18.2% | R926R (CGC→CGT) | hrpA → | ATP‑dependent helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,487,609 | 0 | C | T | 18.2% | 129.1 / 20.6 | 55 | R926R (CGC→CGT) | hrpA | ATP‑dependent helicase |
| Reads supporting (aligned to +/- strand): ref base C (27/18); new base T (5/5); total (32/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACC > W3110S.gb/1487540‑1487679 | aaaCTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGcgcg < 1:1724403/71‑1 (MQ=255) aaCTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGa < 1:4095863/71‑1 (MQ=255) cTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGTGAGt > 1:4109184/1‑71 (MQ=255) cTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGTGAGt > 1:691034/1‑71 (MQ=255) gAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTAcggcg < 1:867143/71‑1 (MQ=255) tttttAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGcgcg < 1:4481079/55‑1 (MQ=255) tttttAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGAc > 1:3397746/1‑71 (MQ=255) ttttAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGAcc > 1:2825930/1‑71 (MQ=255) tttAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGAc > 1:1547810/1‑69 (MQ=255) tttAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCg > 1:1607832/1‑71 (MQ=255) cgcgTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTAc > 1:1660699/1‑71 (MQ=255) gTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGt > 1:2591727/1‑71 (MQ=255) tCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGtt < 1:2714142/46‑1 (MQ=255) tCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGAc < 1:2690386/58‑1 (MQ=255) tCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGtt < 1:853219/71‑1 (MQ=255) aaaCCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAg > 1:3713331/1‑42 (MQ=255) aaaCCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAg > 1:3389756/1‑42 (MQ=255) aaaCCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAg > 1:1205921/1‑42 (MQ=255) aaaCCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAg > 1:1590736/1‑42 (MQ=255) aaaCCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTAc > 1:4037274/1‑46 (MQ=255) aCCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGAcc > 1:2367108/1‑71 (MQ=255) ccGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAg > 1:940604/1‑39 (MQ=255) cGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGt < 1:748117/39‑1 (MQ=255) cGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGt < 1:922156/39‑1 (MQ=255) cGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGt < 1:604973/39‑1 (MQ=255) cGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACcgc > 1:2767956/1‑71 (MQ=255) cGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACcgc > 1:909796/1‑71 (MQ=255) gCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTAcg < 1:2006433/42‑1 (MQ=255) cTGGAGTTACCGTTGCTCGACAGCCTTGAGCGTGAGTTACGGCGGATGACCGGCGTTACCGTTGACcgcg > 1:4235338/1‑70 (MQ=255) cTGGAGTTACCGTTGCTCGACAGCCTTGAGCGTGAGTTACGGCGGATGACCGGCGTTACCGTTGACcgcg > 1:1475610/1‑70 (MQ=255) cTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTAc < 1:3207558/59‑1 (MQ=255) gAGTTACCGTTGCTCGACAGCCTTGAGCGTGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGaa < 1:1669291/69‑1 (MQ=255) gAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGa < 1:2688524/71‑1 (MQ=255) ttACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGaa > 1:3190481/1‑66 (MQ=255) ttACCGTTGCTCGACAGCCTTGAGAGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGaa > 1:1056916/1‑66 (MQ=255) tACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAg < 1:83395/66‑1 (MQ=255) aCCGTTGCTCGACAGCCTTGAGCGTGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTgg > 1:2695506/1‑70 (MQ=255) ccGTTGCTCGACAGCCTT‑AGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCAc < 1:2770018/71‑1 (MQ=255) cGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCAc < 1:4339366/71‑1 (MQ=255) cGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGAACGCGAAGACTGGCAc < 1:1852781/71‑1 (MQ=255) gTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACt < 1:998492/71‑1 (MQ=255) tGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGa > 1:464978/1‑61 (MQ=255) gCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTg > 1:2997340/1‑50 (MQ=255) gCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTgg > 1:3484841/1‑70 (MQ=255) cTCGACAGCCTTGAGCGTGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTggg < 1:2902095/70‑1 (MQ=255) tCGACAGCCTTGAGCGCGAGATACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGAt > 1:3335103/1‑71 (MQ=255) cGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGAt > 1:2333660/1‑70 (MQ=255) cGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATc > 1:619338/1‑71 (MQ=255) cGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATc > 1:3720396/1‑71 (MQ=255) gACAGCCTTGAGCGTGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATc < 1:468041/70‑1 (MQ=255) gACAGCCTTGAGCGTGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCa < 1:2696987/71‑1 (MQ=255) gACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGAt < 1:3548589/69‑1 (MQ=255) cAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCa > 1:4434588/1‑69 (MQ=255) tGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGccc > 1:2732523/1‑70 (MQ=255) taGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCg < 1:4385826/69‑1 (MQ=255) gAGCGTGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCg < 1:739839/70‑1 (MQ=255) gAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCACGa > 1:1128586/1‑71 (MQ=255) gcgAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGa > 1:3038793/1‑43 (MQ=255) cgAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCAcc < 1:4644908/71‑1 (MQ=255) cgAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCAcc < 1:3876571/71‑1 (MQ=255) cgAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCAcc > 1:232812/1‑71 (MQ=255) cgAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATAAGGTGCCCGATCAcc > 1:1750863/1‑71 (MQ=255) | AAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACC > W3110S.gb/1487540‑1487679 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |