| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,489,179 | T→C | 21.8% | F77F (TTT→TTC) | ydcF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,489,179 | 0 | T | C | 21.8% | 141.4 / 37.3 | 72 | F77F (TTT→TTC) | ydcF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (24/32); new base C (8/7); total (33/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01 | |||||||||||
CATGTAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGGCAGAGCAGAAGCGAC > W3110S.gb/1489109‑1489247 | cATGTAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACtttt < 1:3192212/71‑1 (MQ=255) aTGTAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACttttt > 1:4158188/1‑71 (MQ=255) tAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg < 1:3403892/70‑1 (MQ=255) tAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg < 1:3538726/70‑1 (MQ=255) tAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg > 1:1815633/1‑70 (MQ=255) tAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg > 1:1732174/1‑70 (MQ=255) tAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg > 1:2957406/1‑70 (MQ=255) tAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg > 1:3325749/1‑70 (MQ=255) tAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg < 1:1112960/70‑1 (MQ=255) tAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTAGACAACTTTCTTg > 1:3421982/1‑70 (MQ=255) aGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGtat < 1:3261484/71‑1 (MQ=255) aGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGtat < 1:286262/71‑1 (MQ=255) aGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGtat < 1:4338747/71‑1 (MQ=255) aGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGta < 1:2485692/70‑1 (MQ=255) cccGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAgcgc < 1:2539955/70‑1 (MQ=255) cgATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACttttt > 1:2725896/1‑56 (MQ=255) gATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTCTTGTATAGCGGCATCGc < 1:807075/71‑1 (MQ=255) cAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTCTTGTATAGCGCCATCGca > 1:3089327/1‑69 (MQ=255) aaGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGtat > 1:4296812/2‑57 (MQ=255) gCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg < 1:3342240/52‑1 (MQ=255) cAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTCTTGTATAGCGCCATCGCACAg < 1:924054/69‑1 (MQ=255) aaaTTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCAt > 1:3184283/1‑71 (MQ=255) aaTTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATc < 1:197511/71‑1 (MQ=255) aTTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACtttt < 1:2229858/45‑1 (MQ=255) ttCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCg < 1:624192/71‑1 (MQ=255) ttCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTCTTGTATAGCGCCATCGCACAGCATCCg > 1:261250/1‑71 (MQ=255) tCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTCTTGta > 1:3275483/1‑48 (MQ=255) tCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTCTTGta > 1:292639/1‑48 (MQ=255) cTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCAc < 1:3957958/71‑1 (MQ=255) cTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTCTTGTATAGCGCCATCGCACAGCATCCGCAc < 1:2868064/71‑1 (MQ=255) tttACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg < 1:2857017/43‑1 (MQ=255) aCTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTac < 1:105746/70‑1 (MQ=255) aCTGATTAGTGGTGGTATAGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTac > 1:3592176/1‑70 (MQ=255) cTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTac < 1:4270661/69‑1 (MQ=255) aTTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAacac < 1:1702818/71‑1 (MQ=255) aTTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAacac < 1:1349508/71‑1 (MQ=255) aTTAGTGGTGGTATCGGTCACTCGACAACTTTCTTGtata > 1:1150502/1‑40 (MQ=255) aTTAGTGGTGGTATCGGTCACTCGACAACTTTCTTGTATAGCGCCATCGCACAGCATCCGCACTacaaca < 1:4452820/70‑1 (MQ=255) aTTAGTGGTGGTATCGGTCACTCGACAACTTTCTTGTATAGCGCCATCGCACAGCATCCGCACTACAacac > 1:4113082/1‑71 (MQ=255) aGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTa < 1:2488775/70‑1 (MQ=255) aGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTa < 1:788635/70‑1 (MQ=255) gtggtgGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCAt > 1:1610947/1‑43 (MQ=255) tggtggTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAg < 1:4136814/49‑1 (MQ=255) gtggtATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCg > 1:1201926/1‑70 (MQ=255) gtggtATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGc < 1:137547/71‑1 (MQ=255) gtggtATCGGTCACTCGACAACTTTCTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCg < 1:3328088/70‑1 (MQ=255) gtggtATCGGTCACTCGACAACTTTCTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCg < 1:3327998/70‑1 (MQ=255) gtATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCAt < 1:3270567/47‑1 (MQ=255) gtATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGcacc < 1:542408/71‑1 (MQ=255) gtATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGcac < 1:946873/70‑1 (MQ=255) aTCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCAt > 1:778857/1‑45 (MQ=255) aTCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGcaccac > 1:1017508/1‑71 (MQ=255) tCGGTCACTCGACAACTTTTTTGTATAGCGCCATCg < 1:1847980/36‑1 (MQ=255) tCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAg < 1:2452753/41‑1 (MQ=255) tCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACt < 1:3618263/51‑1 (MQ=255) tCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACt < 1:1261838/71‑1 (MQ=255) ggTCACTCGACAACTTTTTTGTATAGCGCCATCGGACAGCAt > 1:3181162/1‑42 (MQ=255) ggTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATc > 1:2753080/1‑43 (MQ=255) ggTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCAc > 1:1032952/1‑48 (MQ=255) ggTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTac < 1:1833164/51‑1 (MQ=255) ggTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATc > 1:2107266/1‑60 (MQ=255) ggTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTgg > 1:2911474/1‑71 (MQ=255) ggTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTgg > 1:4024399/1‑71 (MQ=255) ggTCACTCGACAACTTTTTTGTATAGCGCCAACGCACAGCATCCGCACTACAACACTATc > 1:1558871/1‑60 (MQ=255) gTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCa > 1:1336547/1‑40 (MQ=255) cACTCGACAACTTTTTTGTATAGCGCCATCGCACAg < 1:586868/36‑1 (MQ=255) cACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTgg > 1:3214061/1‑68 (MQ=255) cACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTgg > 1:551018/1‑68 (MQ=255) aCTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTgg < 1:510116/67‑1 (MQ=255) aCTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGgca > 1:3408586/1‑69 (MQ=255) aCTCGACAACTTTCTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGgcag < 1:1635102/70‑1 (MQ=255) cGACAACTTTCTTGTATAGCGCCATCGCACAGCATCCGCa > 1:2690239/1‑40 (MQ=255) gACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTacaaca > 1:3846094/1‑47 (MQ=255) gACAACTTTATTGTATAGCGCCATCGCAAAGCATCCGCACTACAACACTATCCGCACCACTGgcagag > 1:1988552/1‑68 (MQ=255) aCAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGgcagagcaga < 1:1833837/71‑1 (MQ=255) cTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGGCAGAGCAGAAGc > 1:2312659/1‑70 (MQ=255) tttcttGTATAGCGCCATCGCACAGCATCCGCACTac < 1:37842/37‑1 (MQ=255) tttttGTATAGCGCCATCGCACAGCATCCGCACTacaac > 1:214557/1‑39 (MQ=255) tttttGTATAGCGCCATCGCACAGCATCCGCACTacaac > 1:2972638/1‑39 (MQ=255) ttttGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGGCAGAGCAGAAGCGAc > 1:183364/1‑70 (MQ=255) ttttGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGGCAGAGCAGAAGCGAc > 1:1224645/1‑70 (MQ=255) | CATGTAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGGCAGAGCAGAAGCGAC > W3110S.gb/1489109‑1489247 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |