Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,526,310 A→G 11.5% V462A (GTT→GCT)  ansP ← L‑asparagine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,526,3100AG11.5% 125.5 / 9.3 52V462A (GTT→GCT) ansPL‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base A (23/23);  new base G (3/3);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

CTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGTATAAGTCCCGTTCGGGTAATCGAACGC  >  W3110S.gb/1526246‑1526374
                                                                |                                                                
cTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGc                                                             <  1:2376806/70‑1 (MQ=255)
cTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGc                                                             <  1:2371456/70‑1 (MQ=255)
   gacgacTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCaa                                                           <  1:4167585/69‑1 (MQ=255)
   gacgacTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCaa                                                           <  1:2639503/69‑1 (MQ=255)
      gacTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATAc                                                      >  1:2534511/1‑71 (MQ=255)
         tGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGa                                                   >  1:3535792/1‑71 (MQ=255)
           gcgcAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATa                                                 <  1:4399684/71‑1 (MQ=255)
              cAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAATc                                              <  1:767212/71‑1 (MQ=255)
                gTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAATc                                              >  1:199117/1‑69 (MQ=255)
                 tGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAGCCAGCAAAATACCGATAATCGGc                                           <  1:1895952/71‑1 (MQ=255)
                 tGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAATCGGc                                           <  1:3738566/71‑1 (MQ=255)
                  gCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATaa                                                >  1:1627442/1‑65 (MQ=255)
                   cTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCATCAAAATACCGATAATCGCCAg                                         >  1:3724522/1‑71 (MQ=255)
                   cTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCaaaa                                                         >  1:3602060/1‑55 (MQ=255)
                   cTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAg                                         >  1:1259553/1‑71 (MQ=255)
                      tgAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAgcgc                                      <  1:1745506/71‑1 (MQ=255)
                      tgAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAgcg                                       <  1:41935/70‑1 (MQ=255)
                       gAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATaa                                                                  >  1:3903760/1‑42 (MQ=255)
                       gAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCa                                                            >  1:2832753/1‑48 (MQ=255)
                        aaTTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAAc                                                                 >  1:1024779/1‑42 (MQ=255)
                         aTTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATa                                                       >  1:3611018/1‑51 (MQ=255)
                          tttCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAAt                                               >  1:4271757/1‑58 (MQ=255)
                            tCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAGCCAGCAAAATACCGATAATCGGCAGCGCCGCGa                                 >  1:2956384/1‑70 (MQ=255)
                            tCACCAACGCCTTTGCGCACACCAAACCAGCCTATAGCCAGCAAAATACCGATAATCGGCAGCGCCGCGa                                 >  1:1645804/1‑70 (MQ=255)
                                cAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGt                             >  1:1363973/1‑70 (MQ=255)
                                   cgcgTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGTATaa                         <  1:4086933/71‑1 (MQ=255)
                                     cgTTTGCGCACACCAAACCAGCCTATAGCCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGTATAAGt                       >  1:3435812/1‑71 (MQ=255)
                                     cgTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGTATAAGt                       <  1:1454081/71‑1 (MQ=255)
                                       tttGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAATCgg                                            <  1:4487391/48‑1 (MQ=255)
                                       tttGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAATCgg                                            <  1:4098599/48‑1 (MQ=255)
                                        ttGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGTATAAGTccc                    >  1:3192548/1‑71 (MQ=255)
                                         tGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAg                                         >  1:2652847/1‑49 (MQ=255)
                                         tGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGTATAAGTCCCg                   >  1:4038246/1‑71 (MQ=255)
                                            gcACACCAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGTATAAGTCCCGTTc                <  1:3283263/71‑1 (MQ=255)
                                              acacCAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGt                             >  1:4254635/1‑56 (MQ=255)
                                               cacCAAACCAGCCTATAGCCAGCAAAATACCGATaa                                                <  1:1955202/36‑1 (MQ=255)
                                               cacCAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGtata                          <  1:1079931/58‑1 (MQ=255)
                                                 ccAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAGcgccg                                    >  1:3572102/1‑46 (MQ=255)
                                                 ccAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAGcgccg                                    >  1:1618335/1‑46 (MQ=255)
                                                 ccAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAGCGCCGCGATACTATaa                         >  1:723528/1‑57 (MQ=255)
                                                    aaCCAGCCTATAACCAGCAAAATACCGATAATCGGCAg                                         >  1:856129/1‑38 (MQ=255)
                                                    aaCCAGCCTATAACCAGCAAAATACCGATAATCGGCAGcgccgc                                   <  1:2568589/44‑1 (MQ=255)
                                                     aCCAGCCTATAACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGTATAAGTCCCGTTCGGGTAATCg       <  1:3838802/71‑1 (MQ=255)
                                                       cAGCCTATAGCCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGTATAAGTCCCGTTCGGGTAATCGaa     <  1:1362810/71‑1 (MQ=255)
                                                       cAGCCTATAACCAGCAAAATACCGATAATCGGCAGCGCCGCGATa                               >  1:689001/1‑45 (MQ=255)
                                                          ccTATAACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGTATAAGTCCCGTTCGGGTAATCGAACGc  >  1:411392/1‑71 (MQ=255)
                                                          ccTATAACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGTATAAGTCCCGTTCGGGTAATCGAACGc  >  1:3218010/1‑71 (MQ=255)
                                                            tataACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGt                             <  1:1683956/42‑1 (MQ=255)
                                                            tataACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGt                             <  1:1673985/42‑1 (MQ=255)
                                                             ataACCAGCAAAATACCGATAATCGGCAGcgccgc                                   <  1:491712/35‑1 (MQ=255)
                                                             ataACCAGCAAAATACCGATAATCGGCAGcgccgc                                   <  1:496164/35‑1 (MQ=255)
                                                             ataACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGtat                           <  1:710389/43‑1 (MQ=255)
                                                              taACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGTATAAGTCCCGTTCGGGTAATCg       <  1:3281253/62‑1 (MQ=255)
                                                                |                                                                
CTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGATAATCGGCAGCGCCGCGATAGTATAAGTCCCGTTCGGGTAATCGAACGC  >  W3110S.gb/1526246‑1526374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: