| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,559,087 | T→C | 13.9% | V222V (GTA→GTG) | ddpF ← | D‑Ala‑D‑Ala transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,559,087 | 0 | T | C | 13.9% | 184.4 / 20.7 | 72 | V222V (GTA→GTG) | ddpF | D‑Ala‑D‑Ala transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (34/28); new base C (5/5); total (39/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01 | |||||||||||
GGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGA > W3110S.gb/1559018‑1559155 | ggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTa > 1:2456439/1‑71 (MQ=255) gaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTAc < 1:722692/71‑1 (MQ=255) gaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTAc < 1:3476058/71‑1 (MQ=255) aaTCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACa < 1:3550900/71‑1 (MQ=255) aataatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGccc > 1:2704397/1‑71 (MQ=255) taatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACa < 1:3385541/61‑1 (MQ=255) taatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATc < 1:74311/64‑1 (MQ=255) atCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTAc > 1:2828265/1‑58 (MQ=255) tATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATa < 1:1298509/65‑1 (MQ=255) tGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCg > 1:1666441/1‑71 (MQ=255) tGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCg > 1:628287/1‑71 (MQ=255) gATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGc > 1:4003454/1‑70 (MQ=255) gATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGc > 1:3838371/1‑70 (MQ=255) tgCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAccc > 1:3273402/1‑71 (MQ=255) cAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGa > 1:1022840/1‑71 (MQ=255) cAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGa < 1:1270310/71‑1 (MQ=255) cAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGa > 1:3243831/1‑71 (MQ=255) ggTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATAc > 1:1812627/1‑54 (MQ=255) gTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCga > 1:3598842/1‑48 (MQ=255) gTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGAt < 1:1477793/51‑1 (MQ=255) gTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCg > 1:4398754/1‑71 (MQ=255) gTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCg > 1:924600/1‑71 (MQ=255) gTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCCACAATCTGCCCGAGATACATCAcc < 1:1283928/59‑1 (MQ=255) gTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCCACAATCTGCCCGAGATACATCAcc < 1:3506755/59‑1 (MQ=255) tGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATc > 1:3801243/1‑69 (MQ=255) ccGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCg < 1:1788286/64‑1 (MQ=255) gTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCgaga < 1:1304903/45‑1 (MQ=255) tCAGCACCTGCTGCGCGTCGCCCAGTTCCACAATCTGcc > 1:2677535/1‑39 (MQ=255) tCAGCACCTGCTGCGCGTCGCCCAGTTCCACAATCTGcc > 1:834002/1‑39 (MQ=255) tcACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCt < 1:3405183/63‑1 (MQ=255) cACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATc < 1:3411901/46‑1 (MQ=255) cACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGAc < 1:2194596/71‑1 (MQ=255) aCCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCa > 1:4102020/1‑64 (MQ=255) ccTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCata < 1:1315642/65‑1 (MQ=255) ccTGCTGCGCGTCGCCCAGTTCCACAATCTGCCCGAGATACATCACCGCCACCCGATCGCt < 1:1958736/61‑1 (MQ=255) tgctgcGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTAt > 1:859183/1‑71 (MQ=255) tgctgcGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTAt > 1:4245036/1‑71 (MQ=255) tgctgcGCGTCGCCCAGTTCCACAATCTGCCCGAGATa < 1:2325808/38‑1 (MQ=255) gctgcGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTAt > 1:4057831/1‑70 (MQ=255) ctgcGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATc < 1:3959011/70‑1 (MQ=255) tgcGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAccc < 1:3512348/49‑1 (MQ=255) tgcGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAccc < 1:352124/49‑1 (MQ=255) tgcGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAccc < 1:4609218/49‑1 (MQ=255) tgcGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAccc < 1:12167/49‑1 (MQ=255) tgcGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAccc < 1:155336/49‑1 (MQ=255) tgcGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCAc > 1:1901369/1‑71 (MQ=255) tgcGCGTCGCCCAGTTCCACAATCTGCCCGAGATACATc < 1:3310125/39‑1 (MQ=255) cgcgTCGCCCAGTTCCACAATCTGCCCGAGATACATCAc > 1:270133/1‑39 (MQ=255) tCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAc > 1:2124137/1‑41 (MQ=255) cGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCa > 1:1863695/1‑39 (MQ=255) gCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACa < 1:2015622/70‑1 (MQ=255) gCCCAGTTCCACAATCTGCCCGAGATAGATCACCGCCACCCGATCGCTCatat > 1:686375/1‑53 (MQ=25) cAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACg < 1:1959327/54‑1 (MQ=255) cAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACg < 1:55748/54‑1 (MQ=255) cAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACg < 1:2017409/54‑1 (MQ=255) gTTCCACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCa > 1:4304456/1‑45 (MQ=255) ttCTACAATCTGCCCGAGATACATCACCGCCACCCg < 1:2002965/36‑1 (MQ=255) ttCTACAATCTGCCCGAGATACATCACCGCCACCCg < 1:125124/36‑1 (MQ=255) ttCTACAATCTGCCCGAGATACATCACCGCCACCCg < 1:1057732/36‑1 (MQ=255) ttCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTAt > 1:1797559/1‑54 (MQ=255) ttCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTAt > 1:1340044/1‑54 (MQ=255) ttCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtgtg < 1:3444061/71‑1 (MQ=255) cTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCata > 1:300314/1‑44 (MQ=255) cTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCata > 1:2698089/1‑44 (MQ=255) cTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGAc > 1:2633415/1‑48 (MQ=255) cTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACg > 1:976086/1‑49 (MQ=255) cTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgaga > 1:3020957/1‑60 (MQ=255) cTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgaga > 1:2546000/1‑60 (MQ=255) cTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgaga > 1:156080/1‑60 (MQ=255) cTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgaga > 1:1211046/1‑60 (MQ=255) cTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGa > 1:3893085/1‑70 (MQ=255) cTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGa > 1:3360136/1‑70 (MQ=255) | GGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGA > W3110S.gb/1559018‑1559155 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |