| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,567,579 | T→C | 15.8% | N313D (AAT→GAT) | yddV ← | predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,567,579 | 0 | T | C | 15.8% | 89.4 / 10.7 | 38 | N313D (AAT→GAT) | yddV | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base T (14/18); new base C (4/2); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01 | |||||||||||
CTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCGGCTTTCAGTTTGGTTTT > W3110S.gb/1567510‑1567648 | cTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTa < 1:1377233/71‑1 (MQ=255) cAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACa > 1:3017287/1‑56 (MQ=255) agagCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATgg > 1:242950/1‑70 (MQ=255) gagCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCATGATGACCATTAAACATGGCGGCACCGATGGaa < 1:1043281/71‑1 (MQ=255) agCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGaaa < 1:3948016/71‑1 (MQ=255) cTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATCAAACa > 1:512055/1‑51 (MQ=255) tCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGt > 1:2223850/1‑69 (MQ=255) cATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTga < 1:543629/70‑1 (MQ=255) cATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTga < 1:4002596/70‑1 (MQ=255) aTCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTgag < 1:1446262/70‑1 (MQ=255) tCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCg < 1:138912/57‑1 (MQ=255) tCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCg < 1:313096/57‑1 (MQ=255) tCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCg < 1:3834313/57‑1 (MQ=255) cTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATggcgg < 1:3993317/48‑1 (MQ=255) tttGAATGAGGCGCTCATAGTCAGGATGACCATTaaa < 1:1176621/37‑1 (MQ=255) tGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGaaa > 1:2929403/1‑55 (MQ=255) aTGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTc > 1:1906908/1‑71 (MQ=255) gcgcTCATAGTCAGGATGACCATCAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGt < 1:1366001/71‑1 (MQ=255) cTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTg > 1:1065866/1‑71 (MQ=255) cTCATAGTCAGGATGACCATCAAACATGGCGGCACCGATGGAAAGTg < 1:1175870/47‑1 (MQ=255) cATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGaaa > 1:4512264/1‑42 (MQ=255) cATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGaaa > 1:4637944/1‑42 (MQ=255) cATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCg > 1:2636707/1‑71 (MQ=255) cATAGTCAGGATGACCATCAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCg > 1:906014/1‑71 (MQ=255) cATAGTCAGGATGACCATCAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCg > 1:3722107/1‑71 (MQ=255) cATAGTCAGGATGACCATCAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCg > 1:3636421/1‑71 (MQ=255) aTAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCg > 1:3023484/1‑70 (MQ=255) aGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGagag > 1:3896053/1‑46 (MQ=255) gTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTgag < 1:2151116/43‑1 (MQ=255) gTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCGGCtt > 1:2355273/1‑71 (MQ=255) gTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCGGCtt > 1:4580841/1‑71 (MQ=255) gTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCGGCtt < 1:2825600/71‑1 (MQ=255) aGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCTCCGTTTGCGGCttt > 1:2577430/1‑69 (MQ=255) ggATGACCATTAAACATGGCGGCACCGATGGAAAGt < 1:2604553/36‑1 (MQ=255) aTGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCGGCTTTCAGtt < 1:1439636/71‑1 (MQ=255) tGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCGGCTTTCAGttt < 1:4457280/71‑1 (MQ=255) ccATTAAACATGGCGGCACCGATGGATAGTGAGAGGGCAATATCTTCGCCGTTTGCGGCTTTCAg < 1:2149046/65‑1 (MQ=255) ccATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCGGCTTTCAGTTTGGt < 1:4313249/71‑1 (MQ=255) ttAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCGGCTTTCAGt < 1:1410423/63‑1 (MQ=255) ttAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCGGCTTTCAGTTTGGtttt < 1:2474604/71‑1 (MQ=255) ttAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCGGCTTTCAGTTTGGtttt < 1:1564633/71‑1 (MQ=255) | CTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCGGCTTTCAGTTTGGTTTT > W3110S.gb/1567510‑1567648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |