| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,594,973 | A→G | 11.1% | pseudogene (807/1401 nt) | ydeU ← | ECK1502:JW1502:b1509; conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,594,973 | 0 | A | G | 11.1% | 136.8 / 9.7 | 54 | pseudogene (807/1401 nt) | ydeU | ECK1502:JW1502:b1509; conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (28/20); new base G (2/4); total (30/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
GTTATCAAACCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTTATAACCCACACGATTAC > W3110S.gb/1594907‑1595043 | gTTATCAAACCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATAc > 1:292930/1‑70 (MQ=255) tATCAAACCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAg > 1:2445010/1‑71 (MQ=255) aTCAAACCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATAcc < 1:1466823/68‑1 (MQ=255) tCAAACCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGt > 1:468623/1‑71 (MQ=255) aaaCCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATAcc < 1:865676/65‑1 (MQ=255) cAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTa < 1:3177782/71‑1 (MQ=255) cAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCCTa < 1:535572/71‑1 (MQ=255) gTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTa > 1:621926/1‑69 (MQ=255) ataCAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGa > 1:3081558/1‑52 (MQ=255) ataCAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGcc > 1:1307695/1‑71 (MQ=255) taCAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGcc < 1:3973215/70‑1 (MQ=255) cAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGa > 1:3561712/1‑49 (MQ=255) cAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACa > 1:1236069/1‑65 (MQ=255) cgcCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGGTACCAGGTcgc > 1:584062/1‑57 (MQ=255) cccAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCt < 1:2179963/70‑1 (MQ=255) ccAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGt < 1:4619734/71‑1 (MQ=255) cAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGt > 1:3741057/1‑70 (MQ=255) cAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTa < 1:1112379/71‑1 (MQ=255) aGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTaa < 1:3886773/71‑1 (MQ=255) cTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGa > 1:2487338/1‑39 (MQ=255) cTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGGTACCAGGTcgcg < 1:3677348/51‑1 (MQ=255) cTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAAcc < 1:3233290/71‑1 (MQ=255) tCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCt < 1:376576/71‑1 (MQ=255) tCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCt < 1:3516886/71‑1 (MQ=255) cAACATAAGCGCCGGTCTTATTCGCATCGTTCTGa > 1:2764939/1‑35 (MQ=255) aaCATAAGCGCCGGTCTTATTCGCATCGTTCTGGTACCa > 1:2885422/1‑39 (MQ=255) aaCATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGc > 1:1002958/1‑68 (MQ=255) aaCATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCt < 1:928388/69‑1 (MQ=255) aTAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATgc > 1:3648827/1‑71 (MQ=255) tAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATgcg > 1:3527397/1‑71 (MQ=255) aGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGcc > 1:4378512/1‑71 (MQ=255) aGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGcc > 1:1723045/1‑71 (MQ=255) aGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAG‑CCAGCGCTGTAACCGCTGATGCGcc > 1:2992317/1‑70 (MQ=255) gcgcCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCt > 1:469277/1‑62 (MQ=255) cgcCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGa > 1:4637621/1‑63 (MQ=255) gcCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGcc > 1:2847759/1‑44 (MQ=255) gcCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGcc > 1:3683579/1‑44 (MQ=255) gcCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGt < 1:4460369/53‑1 (MQ=255) gcCGGTCTTATTCGCATCGTTCTGATACCACGTcg > 1:4465699/1‑35 (MQ=255) cGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATcc < 1:2327962/71‑1 (MQ=255) gTCTTATTCGCATCGTTCTGGTACCAGGTCGCGTACAGCCCAgcg < 1:2500458/45‑1 (MQ=255) tCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCt < 1:2376435/55‑1 (MQ=255) cTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGAtt > 1:3220947/1‑71 (MQ=255) ttATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAgcg < 1:3612605/42‑1 (MQ=255) tATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTta > 1:932661/1‑71 (MQ=255) tATTCGCATCGTTCTGATACCAGGTCGCGTACAGCACAGCGCTGTAAACGCTGATGCGCCCATCCGATTta > 1:3115163/1‑71 (MQ=255) ttCGCATCGTTCTGGTACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTtata < 1:3476873/71‑1 (MQ=255) ttCGCATCGTTCTGGTACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTtata < 1:469957/71‑1 (MQ=255) ttCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAg > 1:3672014/1‑37 (MQ=255) ttCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAg > 1:3204217/1‑37 (MQ=255) ttCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTtata < 1:3239930/71‑1 (MQ=255) ttCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTtat > 1:2611081/1‑70 (MQ=255) cGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTa > 1:2997370/1‑42 (MQ=255) tCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTTATAACCca > 1:1785296/1‑70 (MQ=255) gTTCTGATACCAGGTCGCGTATAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTTATAACCCacac < 1:720592/71‑1 (MQ=255) gTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTTATAACCCacac < 1:1609266/71‑1 (MQ=255) cTGATACCAGGTCGCGTACAGGCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTTATAACCCACACGAt > 1:1766845/1‑71 (MQ=255) cTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCt > 1:1549409/1‑39 (MQ=255) aTACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTTATAACCCACACGATTAc < 1:4434336/71‑1 (MQ=255) | GTTATCAAACCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTTATAACCCACACGATTAC > W3110S.gb/1594907‑1595043 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |