| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,621,163 | A→G | 24.2% | Q110Q (CAA→CAG) | marR → | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,621,163 | 0 | A | G | 24.2% | 56.1 / 17.5 | 33 | Q110Q (CAA→CAG) | marR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (15/10); new base G (5/3); total (20/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGC > W3110S.gb/1621108‑1621231 | aCAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGt > 1:4199753/1‑70 (MQ=255) cAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAGTGCCATCAATTAGTTg < 1:3751737/71‑1 (MQ=255) cggcgTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAGTGCCATCAATTAGTTGGCCAgg < 1:4197133/71‑1 (MQ=255) tACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATc < 1:3821761/51‑1 (MQ=255) cTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCa > 1:1614055/1‑71 (MQ=255) gTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACaa > 1:4549737/1‑39 (MQ=255) aaaCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGcc < 1:850937/55‑1 (MQ=255) aaaCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGAcc > 1:1317777/1‑61 (MQ=255) aaCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGaa < 1:518170/71‑1 (MQ=255) cTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAAt > 1:1766395/1‑70 (MQ=255) accaccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAg > 1:1999620/1‑51 (MQ=255) accaccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAg > 1:2664372/1‑51 (MQ=255) accaccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTa < 1:485542/69‑1 (MQ=255) accaccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTa < 1:3029745/69‑1 (MQ=255) ccaccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGcc < 1:1234087/48‑1 (MQ=255) caccGGCGGCGCGGCAATATGTGAACAGTGCCATCAATTAGTTGGCCAGGACCTGCACCAAGaa > 1:271857/1‑64 (MQ=255) accGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAg > 1:4544843/1‑48 (MQ=255) gcggcgcggcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAc < 1:1600484/71‑1 (MQ=255) ggcgcggcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCAcc > 1:3758008/1‑52 (MQ=255) cgcggcAATATGTGAACAATGCCATCAATTAGTTGGc < 1:2085113/37‑1 (MQ=255) gcggcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGaa > 1:2466224/1‑54 (MQ=255) ggcAATATGTGAACAGTGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcgg < 1:1956846/71‑1 (MQ=255) ggcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcgg < 1:1992100/71‑1 (MQ=255) gcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcggc > 1:3739499/1‑71 (MQ=255) atGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTaa > 1:95742/1‑50 (MQ=255) tgtgAACAGTGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACg > 1:3716586/1‑70 (MQ=255) gtgAACAGTGCCATCAATTAGTTGGCCAGGACCTGCACCAAGaa > 1:4017180/1‑44 (MQ=255) gtgAACAGTGCCATCAATTAGTTGGCCAGGACCTGCACCAAGaa > 1:3640196/1‑44 (MQ=255) gtgAACAGTGCCATCAATTAGTTGGCCAGGACCTGCACCAAGaa > 1:1183394/1‑44 (MQ=255) tgAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAc > 1:3255080/1‑55 (MQ=255) gAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAc > 1:3693042/1‑54 (MQ=255) aaCAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGa > 1:486757/1‑57 (MQ=255) aaCAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGa > 1:604226/1‑57 (MQ=255) cAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGc < 1:4125887/71‑1 (MQ=255) | ACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGC > W3110S.gb/1621108‑1621231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |