Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,512,687 Δ1 bp 100% coding (654/1248 nt) ypdG ← predicted enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,512,6870G.77.8% 30.7 / 13.7 18coding (654/1248 nt)ypdGpredicted enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base G (4/0);  new base . (2/12);  total (6/12)
Fisher's exact test for biased strand distribution p-value = 4.90e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

CTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAA  >  W3110S.gb/2512633‑2512736
                                                      |                                                 
ctcACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACgtag                                    >  1:1078561/1‑70 (MQ=255)
ctcACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACgtag                                    >  1:1133417/1‑70 (MQ=255)
ctcACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACgtag                                    >  1:913205/1‑70 (MQ=255)
ctcACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACgtag                                    >  1:8376/1‑70 (MQ=255)
ctcACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACgtag                                    <  1:1124109/69‑1 (MQ=255)
ctcACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACgtag                                    <  1:955629/69‑1 (MQ=255)
ctcACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACgtag                                    <  1:624597/69‑1 (MQ=255)
ctcACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACgtag                                    <  1:36507/69‑1 (MQ=255)
ctcACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACgtag                                    <  1:1174230/69‑1 (MQ=255)
                aGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGTGTGATGACGTAGTACATCGCCAGCATGAc                   >  1:1713590/1‑70 (MQ=255)
                aGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGAc                   >  1:1125386/1‑70 (MQ=255)
                 gCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACgtag                                    <  1:666321/52‑1 (MQ=255)
                                     ttGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGaa  <  1:2497761/66‑1 (MQ=255)
                                     ttGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGaa  <  1:312774/66‑1 (MQ=255)
                                     ttGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGaa  <  1:441390/66‑1 (MQ=255)
                                     ttGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGaa  <  1:505671/66‑1 (MQ=255)
                                     ttGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGaa  <  1:1666377/66‑1 (MQ=255)
                                     ttGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGaa  <  1:1246695/66‑1 (MQ=255)
                                                      |                                                 
CTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAA  >  W3110S.gb/2512633‑2512736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑